Slab-gel and capillary electrophoretic characterization of polyamidoamine dendrimers

Abstract Polyamidoamine (PAMAM) dendrimers and derivatives of these polymers were analyzed by polyacrylamide gel- (PAGE) and capillary (CE) electrophoretic methods. Slab-gel PAGE allowed characterization of several generations of PAMAM dendrimers with terminal amino or carboxylate groups. Generational separation was achieved in CE up to the fifth generation of ammonia-core PAMAM dendrimers. Furthermore, CE allowed the separation of different substitution levels of low-generation, PAMAM dendrimers in which terminal amino groups were modified with salicylaldehyde groups, or of low-generation, methyl ester PAMAM dendrimers in which some of the terminal methyl ester groups were hydrolyzed to carboxylate groups.

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