Polarographic Investigations of Vitamin C. III. Reduction Waves of the Condensation Products of Dehydro-L-ascorbic Acid and its Related Compounds with o-Phenylenediamine. Application to the Simultaneous Determination of L-Ascorbic Acid, Dehydro-L-ascorbic Acid and their Related Compounds

The polarographic behavior of dehydro-L-ascorbic acid (DAA), dehydroreductic acid (DRA), alloxan, mesoxalaldehyde (MOA) and 2,3-diketogulonic acid (DGA) in acid buffer solutions containing o-phenylenediamine(OPD), has been investigated.DAA shows three diffusion controlled reduction waves, whose half-wave potentials(E1⁄2) at pH 3.60 are −0.26, −0.44 and −0.67 V. (vs. N. C. E.), respectively. The limiting currents of the three waves depend on the time after the addition of OPD into the electrolytic solution, the concentration of OPD. pH and the temperature. When the electrolytic solution contains both AA and DAA, the oxidation wave of L-ascorbic acid (AA) can be obtained independently of the three waves for DAA in one polarogram.DGA shows two reduction waves whose E1⁄2 at pH 3.44 are −0.27 and −0.40 V., respectively. The E1⁄2 of the first wave is very close to that of the first wave for DAA and the second wave seems to be identical with the second wave for DAA, but the velocities of the condensation reactio...

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