Improved speed, specificity, and limit of determination of an aqueous acid extraction thiobarbituric acid-C18 method for measuring lipid peroxidation in beef

The objectives of this study were to improve the speed, specificity, and limit of determination of the aqueous acid extraction thiobarbituric acid (TBA) method for measuring malonaldehyde (MA) as a marker of lipid peroxidation in ground beef. The TBA reaction time was reduced from 30 to 5 min at 94±1 o C by increasing the concentration of TBA from 20 to 80 mM. The TBA-reactive substances (TBARS) in ground beef extracts and MA standard solution were stable for up to 2 and 12 days, respectively, in 5% (w/v) trichloroacetic acid (TCA) at 4 o C