Metabolic significance of indoleacetylaspartic acid in developing cotton leaves

The formation of indoleacetylaspartic acid (IAAsp) and its metabolic significance were studied in the developing cotyledons and primary two leaves of cotton (Gossypium barbadense L. cv. Giza 68, long staple). Levels of indoleacetic acid (IAA), IAAsp, bound IAA and degradation of tryptophan (Trp)-carboxyl-[14C] by enzyme extracts were estimated from leaf emergence to full expansion. The patterns were also followed in the two cotyledons before and after germination. The results suggest synthesis of IAAsp in response to either active Trp-conversion or hydrolysis of bound IAA. Ester IAA accumulating during leaf expansion, or other bound forms of IAA initially stored in the cotyledons may be the substances hydrolysed. IAAsp appears to be accumulated during phases of low demand and to be exhausted during phases of high demand on IAA. Synthesis and hydrolysis of the conjugate may, therefore, influence the steady state concentration of the hormone during leaf development.

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