The potential anti-proliferative effect of β-sitosterol on human mast cell line-1 cells.

Thymic stromal lymphopoietin (TSLP) was reported to induce mast cell proliferation and aggravate allergic reactions through activation of mouse double minute 2 (MDM2). We aimed to ascertain that β-sitosterol (SI), which is one of the several phytosterols found mostly in foods, would regulate TSLP-induced mast cell proliferation. The results showed that SI significantly decreased the proliferation of human mast cell line (HMC-1) cells promoted by TSLP. SI significantly decreased the mRNA expression of Ki-67 in the TSLP-treated HMC-1 cells. SI significantly suppressed the production and mRNA expression of interleukin-13 in the TSLP-treated HMC-1 cells. Furthermore, SI downregulated the expression of MDM2 and phosphorylation of STAT6, whereas it upregulated the expression of p53, activation of caspase-3, and cleavage of poly ADP-ribose polymerase in the TSLP-treated HMC-1 cells. Results of this study suggest that SI may be a potential therapeutic agent for mast cell-mediated allergic diseases.

[1]  J. S. Jiménez,et al.  Asthma and insulin resistance in obese children and adolescents , 2014 .

[2]  Na‐Ra Han,et al.  TSLP induces mast cell development and aggravates allergic reactions through the activation of MDM2 and STAT6. , 2014, The Journal of investigative dermatology.

[3]  T. Myers,et al.  TNF-α/IL-17 synergy inhibits IL-13 bioactivity via IL-13Rα2 induction. , 2014, The Journal of allergy and clinical immunology.

[4]  S. Costantini,et al.  Carotid Intima-Media Thickness Is Predicted by Combined Eotaxin Levels and Severity of Hepatic Steatosis at Ultrasonography in Obese Patients with Nonalcoholic Fatty Liver Disease , 2014, PloS one.

[5]  Hyung‐Min Kim,et al.  An alternative method to reduce anaphylaxis by moxibustion , 2014 .

[6]  S. Saeidnia,et al.  Anti‐proliferative and apoptotic activities of constituents of chloroform extract of Juglans regia leaves , 2014, Cell proliferation.

[7]  Na‐Ra Han,et al.  The β-sitosterol attenuates atopic dermatitis-like skin lesions through down-regulation of TSLP , 2014, Experimental biology and medicine.

[8]  M. Pellegrino,et al.  The Epithelial Cell-Derived Atopic Dermatitis Cytokine TSLP Activates Neurons to Induce Itch , 2013, Cell.

[9]  A. Caraffa,et al.  Impact of Mast Cells on the Skin , 2013, International journal of immunopathology and pharmacology.

[10]  C. Anuradha,et al.  Nitric oxide mediates the insulin sensitizing effects of β-sitosterol in high fat diet-fed rats. , 2013, Nitric oxide : biology and chemistry.

[11]  Chunsheng Zhu,et al.  MicroRNA-143 Downregulates Interleukin-13 Receptor Alpha1 in Human Mast Cells , 2013, International journal of molecular sciences.

[12]  H. Anders,et al.  p53-independent roles of MDM2 in NF-κB signaling: implications for cancer therapy, wound healing, and autoimmune diseases. , 2012, Neoplasia.

[13]  Wenqiang Cao,et al.  Ruthenium complexes containing 2,6-bis(benzimidazolyl)pyridine derivatives induce cancer cell apoptosis by triggering DNA damage-mediated p53 phosphorylation. , 2012, Dalton transactions.

[14]  H. Anders,et al.  MDM2 (murine double minute-2) links inflammation and tubular cell healing during acute kidney injury in mice. , 2012, Kidney international.

[15]  C. Loddenkemper,et al.  Dietary docosahexaenoic acid in combination with arachidonic acid ameliorates allergen‐induced dermatitis in mice , 2011, Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology.

[16]  W. Leonard,et al.  IL-13 Induces Skin Fibrosis in Atopic Dermatitis by Thymic Stromal Lymphopoietin , 2011, The Journal of Immunology.

[17]  J. Kolls,et al.  Human TH17 cells express a functional IL-13 receptor and IL-13 attenuates IL-17A production. , 2011, The Journal of allergy and clinical immunology.

[18]  Yaojiong Wu,et al.  Molecular regulation of mast cell development and maturation , 2010, Molecular Biology Reports.

[19]  R. Geha,et al.  Thymic stromal lymphopoietin , 2010, Annals of the New York Academy of Sciences.

[20]  S. Ziegler,et al.  Thymic stromal lymphopoietin and the pathophysiology of atopic disease , 2009, Expert review of clinical immunology.

[21]  Do Hyung Kim,et al.  Glycoproteomic analysis of plasma from patients with atopic dermatitis: CD5L and ApoE as potential biomarkers , 2008, Experimental & Molecular Medicine.

[22]  Shaomeng Wang,et al.  Targeting the MDM2-p53 Interaction for Cancer Therapy , 2008, Clinical Cancer Research.

[23]  Q. Yuan,et al.  IL-4/Stat6 activities correlate with apoptosis and metastasis in colon cancer cells. , 2008, Biochemical and biophysical research communications.

[24]  S. Ziegler,et al.  Thymic stromal lymphopoietin is released by human epithelial cells in response to microbes, trauma, or inflammation and potently activates mast cells , 2007, The Journal of experimental medicine.

[25]  C. Brightling,et al.  Mast cells express IL‐13Rα1: IL‐13 promotes human lung mast cell proliferation and FcɛRI expression , 2006, Allergy.

[26]  S. Galli,et al.  Interleukin‐4‐triggered, STAT6‐dependent production of a factor that induces mouse mast cell apoptosis , 2006, European journal of immunology.

[27]  Johannes Gerdes,et al.  Ki‐67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells , 2006, Journal of cellular physiology.

[28]  A. Awad,et al.  Effect of resveratrol and beta-sitosterol in combination on reactive oxygen species and prostaglandin release by PC-3 cells. , 2005, Prostaglandins, leukotrienes, and essential fatty acids.

[29]  Yanan Yang,et al.  Caspase-dependent apoptosis and -independent poly(ADP-ribose) polymerase cleavage induced by transforming growth factor beta1. , 2004, The international journal of biochemistry & cell biology.

[30]  Melissa A Brown,et al.  The role of mast cells in allergy and autoimmunity. , 2002, Current opinion in immunology.

[31]  S. S. Timoshin,et al.  Proliferative Processes in the Epidermis of Patients with Atopic Dermatitis Treated with Thymodepressin , 2002, Bulletin of Experimental Biology and Medicine.

[32]  T. Hasegawa,et al.  Differential effect of phosphodiesterase inhibitors on IL‐13 release from peripheral blood mononuclear cells , 2001, Clinical and experimental immunology.

[33]  M. Manns,et al.  Human Endothelial Cells Regulate Survival and Proliferation of Human Mast Cells , 2000, The Journal of experimental medicine.

[34]  C. De-Fraja,et al.  Upregulation and activation of Stat6 precede vascular smooth muscle cell proliferation in carotid artery injury model. , 2000, Arteriosclerosis, thrombosis, and vascular biology.

[35]  K. Deichmann,et al.  Atopy and asthma: genetic variants of IL-4 and IL-13 signalling , 2000 .

[36]  P. Bouic,et al.  Plant sterols and sterolins: a review of their immune-modulating properties. , 1999, Alternative medicine review : a journal of clinical therapeutic.

[37]  H. Sugiura,et al.  Mitosis of mast cells in skin lesions of atopic dermatitis. , 1993, Acta dermato-venereologica.

[38]  G. Sauter,et al.  MDM2 amplification is an independent prognostic feature of node-negative, estrogen receptor-positive early-stage breast cancer. , 2010, Cancer biomarkers : section A of Disease markers.

[39]  Y. Okayama,et al.  Development, migration, and survival of mast cells , 2006, Immunologic research.

[40]  F. Kuipers,et al.  Dietary plant sterols accumulate in the brain. , 2006, Biochimica et biophysica acta.

[41]  W. Leonard,et al.  Jaks and STATs: biological implications. , 1998, Annual review of immunology.