Development of a trimethylamine gas biosensor system

Abstract A trimethylamine (TMA) gas biosensor was developed, based on the immobilized mould, Penicillium decumbens , an oxygen electrode, a flow cell, a peristaltic pump, a recorder and a buffer tank. The optimum conditions for the sensor were: pH 7·6, temperature 30°C, flow rate 0·3 ml/min, and sample volume 50 μl. Upon measurement of TMA concentrations in fish muscle, a good correlation was found between results obtained from the sensor and those obtained from gas-liquid chromatography (GLC). The correlation coefficient was 0·84. Each assay lasted 30 min. The proposed sensor was stable even after 37 days.

[1]  W. J. Dyer.  Amines in Fish Muscle: I. Colorimetric Determination of Trimethylamine as the Picrate Salt , 1945 .

[2]  J. R. Burt,et al.  Automated Analysis of Hypoxanthine , 1965 .

[3]  E. Watanabe,et al.  Development of biosensor for the determination of total viable bacterial cell count. , 1991 .

[4]  Tuneyuki Saitô.  ADENOSINETRIPHOSPHATE AND THE RELATED COMPOUNDS IN THE MUSCLES OF AQUATIC ANIMALS , 1961 .

[5]  Ken-ichi Arai,et al.  A new method for estimating the freshness of fish , 1959 .

[6]  Y. Shimizu,et al.  Semiconductive trimethylamine gas sensor for detecting fish freshness , 1991 .

[7]  H. Yamanaka,et al.  Changes in non-volatile amine contents of the meats of sardine and saury pike during storage. , 1986 .

[8]  Junsaku Nonaka,et al.  Determination of Volatile Amines in Fish Muscle by Gas-Liquid Chromatography-I , 1967 .

[9]  W. M. Ashton,et al.  Coumarin and related compounds of Anthoxanthum puelii and Melilotus alba and dicoumarol formation in spoilt sweet vernal and sweet clover hay , 1964 .

[10]  Munehiko Tanaka,et al.  Microbial Sensors for the Detection of Fish Freshness , 1987 .

[11]  H. Uchiyama,et al.  The Current Studies on the Freshness of Fish with Special Reference to Nucleic Acids and their Related Compounds , 1970 .

[12]  T. Gill,et al.  Rapid, Automated Analysis of Amines in Seafood by Ion‐Moderated Partition HPLC , 1984 .

[13]  K. Arai,et al.  Changes in Adenine Nucleotides in the Muscles of Some Marine Invertebrates , 1961, Nature.

[14]  Hiroshi Suzuki,et al.  Relations between Freshness and Behavior of ATP Related Substances in Chicken Muscle , 1981 .

[15]  C. K. Murray,et al.  Rapid estimations of hypoxanthine concentrations as indices of the freshness of chill‐stored fish , 1964 .

[16]  S. A. Beatty,et al.  The Measurement of Spoilage in Fish , 1937 .