Preventive effects of transplantation of oral mucosal epithelial cells seeded on a decellularized amniotic membrane in a model of intrauterine adhesion.

OBJECTIVE To observe the preventive effects of oral mucosal epithelial cells (OMECs) seeded on a decellularized human amniotic membrane (dHAM) in a rat model of intrauterine adhesion (IUA). METHODS IUAs were established by mechanical endometrial scraping in model rats. Thirty-six Sprague-Dawley rats were divided into: IUA, dHAM transplantation, and dHAM+OMECs transplantation groups (12 in each group). The oral mucosae of rats were collected, and the OMECs were cultured on the dHAM. The dHAM and dHAM+OMECs were transplanted into left scraped uteri (right uteri as controls) in the dHAM and dHAM+OMECs transplantation groups. At 3, 7, 14, and 28 days after transplantation, the uterine samples underwent histological and immunohistochemical staining. RESULTS After endometrial scraping, the endometria and uterine cavities were obliterated. The ratios of the fibrotic areas to the whole endometrium in the IUA and dHAM transplantation group were higher than in the control group (P<0.01). In the dHAM+OMECs transplantation group, the fibrotic area significantly decreased compared to the IUA group after 7, 14, and 28 days, and the dHAM transplantation group at 14 and 28 days (P<0.01). At 14 and 28 days after surgery in the dHAM+OMECs transplantation group, the number of endometrial glands and the endometrial thickness increased (P<0.01), and regenerated epithelia were observed. Immunohistochemical assays for cytokeratin 18 demonstrated that the newly-regenerated epithelium was endometrium. CONCLUSION OMECs can enhance repair of damaged endometrium, and promise to be novel and effective in preventing IUAs. However, studies of OMECs in patients with IUA need further exploration.

[1]  H. Duan,et al.  Efficacy of freeze‐dried amnion graft following hysteroscopic adhesiolysis of severe intrauterine adhesions , 2017, International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics.

[2]  C. Deeken,et al.  Type I/type III collagen ratio associated with diverticulitis of the colon in young patients. , 2017, The Journal of surgical research.

[3]  Yan Gu,et al.  Application of Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Intrauterine Adhesions in Rats , 2016, Cellular Physiology and Biochemistry.

[4]  M. Uiprasertkul,et al.  Long-term result of autologous cultivated oral mucosal epithelial transplantation for severe ocular surface disease , 2016, Cell and Tissue Banking.

[5]  H. Kurita,et al.  Construction and characterization of human oral mucosa equivalent using hyper-dry amniotic membrane as a matrix. , 2016, Archives of oral biology.

[6]  C. Gargett,et al.  Endometrial stem/progenitor cells: the first 10 years , 2015, Human reproduction update.

[7]  Yabin Zhu,et al.  Biocompatible surgical meshes based on decellularized human amniotic membrane. , 2015, Materials science & engineering. C, Materials for biological applications.

[8]  S. Kinoshita,et al.  Autologous Transplantation of Oral Mucosal Epithelial Cell Sheets Cultured on an Amniotic Membrane Substrate for Intraoral Mucosal Defects , 2015, PloS one.

[9]  S. French,et al.  Carrier-free cultured autologous oral mucosa epithelial cell sheet (CAOMECS) for corneal epithelium reconstruction: a histological study. , 2015, The ocular surface.

[10]  Jiří Kanta,et al.  Collagen matrix as a tool in studying fibroblastic cell behavior , 2015, Cell adhesion & migration.

[11]  T. Okano,et al.  Preventive effect of oral mucosal epithelial cell sheets on intrauterine adhesions. , 2015, Human reproduction.

[12]  K. Nishida,et al.  Maintenance and Distribution of Epithelial Stem/Progenitor Cells after Corneal Reconstruction Using Oral Mucosal Epithelial Cell Sheets , 2014, PloS one.

[13]  M. Lako,et al.  Successful Application of Ex Vivo Expanded Human Autologous Oral Mucosal Epithelium for the Treatment of Total Bilateral Limbal Stem Cell Deficiency , 2014, Stem cells.

[14]  T. Okano,et al.  Evidence of the survival of ectopically transplanted oral mucosal epithelial stem cells after repeated wounding of cornea. , 2014, Molecular therapy : the journal of the American Society of Gene Therapy.

[15]  H. Taylor,et al.  Bone Marrow-Derived Stem Cell (BMDSC) Transplantation Improves Fertility in a Murine Model of Asherman's Syndrome , 2014, PloS one.

[16]  F. Xiao,et al.  Etiology, treatment, and reproductive prognosis of women with moderate‐to‐severe intrauterine adhesions , 2014, International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics.

[17]  R. Redmond,et al.  The clinical applications of human amnion in plastic surgery. , 2014, Journal of plastic, reconstructive & aesthetic surgery : JPRAS.

[18]  Songying Zhang,et al.  A comparison of intrauterine balloon, intrauterine contraceptive device and hyaluronic acid gel in the prevention of adhesion reformation following hysteroscopic surgery for Asherman syndrome: a cohort study. , 2013, European journal of obstetrics, gynecology, and reproductive biology.

[19]  Hongbin Li,et al.  Urethral reconstruction with tissue engineering and RNA interference techniques in rabbits. , 2013, Urology.

[20]  Hideo Namiki,et al.  Prevention of esophageal stricture after endoscopic submucosal dissection using tissue-engineered cell sheets ( , 2013 .

[21]  C. March.  Management of Asherman's syndrome. , 2011, Reproductive biomedicine online.

[22]  T. Okano,et al.  Bladder Augmentation Using Tissue-Engineered Autologous Oral Mucosal Epithelial Cell Sheets Grafted on Demucosalized Gastric Flaps , 2011, Transplantation.

[23]  K. Roy.  Reproductive Outcome Following Hysteroscopic Adhesiolysis in Patients with Infertility Due to Asherman's Syndrome , 2010 .

[24]  M. Amer,et al.  Human amnion as a temporary biologic barrier after hysteroscopic lysis of severe intrauterine adhesions: pilot study. , 2010, Journal of minimally invasive gynecology.

[25]  R. Kimble,et al.  A randomised controlled trial of amniotic membrane in the treatment of a standardised burn injury in the merino lamb. , 2009, Burns : journal of the International Society for Burn Injuries.

[26]  Y. Cheong,et al.  Asherman syndrome--one century later. , 2008, Fertility and sterility.

[27]  T. Li,et al.  Factors affecting reproductive outcome of hysteroscopic adhesiolysis for Asherman's syndrome. , 2008, Fertility and sterility.

[28]  大木 岳志,et al.  Treatment of oesophageal ulcerations using endoscopic transplantation of tissue-engineered autologous oral mucosal epithelial cell sheets in a canine model , 2006 .

[29]  N. Koizumi,et al.  Comparison of intact and denuded amniotic membrane as a substrate for cell-suspension culture of human limbal epithelial cells , 2006, Graefe's Archive for Clinical and Experimental Ophthalmology.

[30]  林田 康隆.  Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo on a temperature-responsive culture surface , 2005 .

[31]  T. Inatomi,et al.  Transplantation of cultivated autologous oral mucosal epithelial cells in patients with severe ocular surface disorders , 2004, British Journal of Ophthalmology.

[32]  T. Okano,et al.  Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. , 2004, The New England journal of medicine.

[33]  N. Koizumi,et al.  Cultivation of corneal epithelial cells on intact and denuded human amniotic membrane. , 2000, Investigative ophthalmology & visual science.

[34]  H. Dua,et al.  Amniotic membrane transplantation for ocular surface reconstruction , 1999, The British journal of ophthalmology.