Determination of dioxins in human hair: Estimation of external and internal exposure to dioxins

ObjectivesTo clarify the origin of dioxin and related compounds (dioxins) in human hair, we determined the amounts of adsorbed dioxins in human hair, and the distribution of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in rats.MethodsHuman hair specimens, packed in a glass column, were exposed to ambient air that was introduced into the column with an air pump for 24 h. Rats were administered TCDD by gavage at doses of 0.2, 0.8, and 1.6 μg/kg body weight. Four weeks after TCDD administration, hair from the back, serum, and adipose tissue were removed under diethyl ether anesthesia. The amounts of dioxins in these samples were analyzed by high resolution gas chromatography with mass spectroscopy.ResultsExposure of the hair specimens to ambient air for one day increased the total toxic equivalent (TEQ) value by 51%. In TCDD-treated rats, the amount of TCDD in hair increased in a dose-dependent manner, and showed a significant positive correlation with that in adipose tissue.ConclusionsHuman hair was found to retain dioxins by both internal and external exposure, and the contribution of external exposure was estimated to be about 40% of the TEQ.

[1]  Teruyuki Nakao,et al.  Assessment of human exposure to PCDDs, PCDFs and Co-PCBs using hair as a human pollution indicator sample I: Development of analytical method for human hair and evaluation for exposure assessment. , 2002, Chemosphere.

[2]  Ji-Hoon Park,et al.  Monitoring of air pollution by polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans of pine needles in Korea. , 2002, Chemosphere.

[3]  W Slob,et al.  A generic toxicokinetic model for persistent lipophilic compounds in humans: an application to TCDD. , 1996, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[4]  M. Sunaga,et al.  Balance of Intake and Excretion of 20 Congeners of Polychlorinated Dibenzo-p-dioxin, Polychlorinated Dibenzofuran and Coplanar Polychlorinated Biphenyl in Healthy Japanese Men , 2001 .

[5]  Safe,et al.  Toxic equivalency factors (TEFs) for PCBs, PCDDs, PCDFs for humans and wildlife. , 1998, Environmental health perspectives.

[6]  R. Wenning,et al.  Principal components analysis of potential sources of polychlorinated dibenzop-dioxin and dibenzofuran residues in surficial sediments from Newark Bay, New Jersey , 1993 .

[7]  N. Nishimura,et al.  Induction of metallothionein in the livers of female Sprague-Dawley rats treated with 2,3,7 ,8-tetrachlorodibenzo-p-dioxin. , 2001, Life sciences.

[8]  C. Tohyama,et al.  Maternal exposure to a low dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) suppressed the development of reproductive organs of male rats: dose-dependent increase of mRNA levels of 5alpha-reductase type 2 in contrast to decrease of androgen receptor in the pubertal ventral prostate. , 2001, Toxicological sciences : an official journal of the Society of Toxicology.

[9]  A. Tsatsakis,et al.  Hair analysis: another approach for the assessment of human exposure to selected persistent organochlorine pollutants. , 2002, Chemosphere.

[10]  T. Puzyn,et al.  Dioxin-like compounds in pine needles around Tokyo Bay, Japan in 1999. , 2004, Journal of environmental monitoring : JEM.

[11]  Karl-Werner Schramm,et al.  Dioxin hair analysis as monitoring pool , 1992 .

[12]  K. Schramm.  Hair: A Matrix for Non-Invasive Biomonitoring of Organic Chemicals in Mammals , 1997, Bulletin of environmental contamination and toxicology.

[13]  M. Morita,et al.  Comparison of PCDD/F and coplanar PCB concentrations in Japanese human adipose tissue collected in 1970-1971, 1994-1996 and 2000. , 2002, Chemosphere.

[14]  Peter Popp,et al.  Application of accelerated solvent extraction followed by gas chromatography, high-performance liquid chromatography and gas chromatography–mass spectrometry for the determination of polycyclic aromatic hydrocarbons, chlorinated pesticides and polychlorinated dibenzo-p-dioxins and dibenzofurans in s , 1997 .

[15]  D. Paustenbach,et al.  Relative distribution of 2,3,7,8-tetrachlorodibenzo-p-dioxin in human hepatic and adipose tissues. , 1990, Toxicology letters.

[16]  Hideaki Miyata,et al.  Formation of dioxin analogs by open-air incineration of waste wood and by fire of buildings and houses concerning Hanshin Great Earthquake in Japan. , 2002, Chemosphere.