Essential role of Ca2+ -dependent phospholipase A2 in estradiol-induced lysosome activation.

The mechanism of lysosome activation by 17beta-estradiol has been studied in mussel blood cells. Cell treatment with estradiol induced a sustained increase of cytosolic free Ca2+ that was completely prevented by preincubating the cells with the Ca2+ chelator BAPTA-AM. Estradiol treatment was also followed by destabilization of the lysosomal membranes, as detected in terms of the lysosomes' increased permeability to neutral red. The effect of estradiol on lysosomes was almost completely prevented by preincubation with the inhibitor of cytosolic Ca2+ -dependent PLA2 (cPLA2), arachidonyl trifluoromethyl ketone (AACOCF3), and was significantly reduced by preincubation with BAPTA-AM. In contrast, it was virtually unaffected by preincubation with the inhibitor of Ca2+ -independent PLA2, (E)-6-(bromomethylene)tetrahydro-3-(1-naphtalenyl)-2H-pyran-2-one (BEL). The Ca2+ ionophore A-23187 yielded similar effects on [Ca2+](i) and lysosomes. Exposure to estradiol also resulted in cPLA2 translocation from cytosol to membranes, lysosome enlargement, and increased protein degradation. These results suggest that the destabilization of lysosomal membranes following cell exposure to estradiol occurs mainly through a Ca2+ -dependent mechanism involving activation of Ca2+ -dependent PLA2. This mechanism promotes lysosome fusion and catabolic activities and may mediate short-term estradiol effects.

[1]  D. Lowe,et al.  Responses of lysosomes in the digestive cells of the common mussel, Mytilus edulis, to sex steroids and cortisol , 1978, Cell and Tissue Research.

[2]  M. Moore.  Cytochemical demonstration of latency of lysosomal hydrolases in digestive cells of the common mussel, Mytilus edulis, and changes induced by thermal stress , 1976, Cell and Tissue Research.

[3]  Thorsten Lang,et al.  Membrane fusion. , 2002, Current opinion in cell biology.

[4]  A. Viarengo,et al.  Single and combined effects of heavy metals and hormones on lysosomes of haemolymph cells from the mussel Mytilus galloprovincialis , 2000 .

[5]  P. Donkin,et al.  Impact of the Sea Empress oil spill on lysosomal stability in mussel blood cells. , 2000, Marine environmental research.

[6]  J. Bender,et al.  Human vascular endothelial cells contain membrane binding sites for estradiol, which mediate rapid intracellular signaling. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[7]  J. Luzio,et al.  Lysosome-endosome fusion and lysosome biogenesis. , 2000, Journal of cell science.

[8]  Wei Li,et al.  Induction of cell death by the lysosomotropic detergent MSDH , 2000, FEBS letters.

[9]  A. Viarengo,et al.  Critical evaluation of an intercalibration exercise undertaken in the framework of the MED POL biomonitoring program. , 2000, Marine environmental research.

[10]  C. Breton,et al.  Estradiol-stimulated nitric oxide release in human granulocytes is dependent on intracellular calcium transients: evidence of a cell surface estrogen receptor. , 2000, Blood.

[11]  E. Levin.  Cellular Functions of the Plasma Membrane Estrogen Receptor , 1999, Trends in Endocrinology & Metabolism.

[12]  W. Cho,et al.  A Structure-Function Study of the C2 Domain of Cytosolic Phospholipase A2 , 1999, The Journal of Biological Chemistry.

[13]  D. Golan,et al.  Estradiol induces the calcium-dependent translocation of endothelial nitric oxide synthase. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[14]  J. Neuzil,et al.  α‐Tocopheryl succinate‐induced apoptosis in Jurkat T cells involves caspase‐3 activation, and both lysosomal and mitochondrial destabilisation , 1999, FEBS letters.

[15]  T. Utsumi,et al.  Activation of caspase‐3‐like protease by digitonin‐treated lysosomes , 1998, FEBS letters.

[16]  K. Öllinger,et al.  Oxidative stress causes relocation of the lysosomal enzyme cathepsin D with ensuing apoptosis in neonatal rat cardiomyocytes. , 1998, The American journal of pathology.

[17]  G. Giese,et al.  Estradiol binding to cell surface raises cytosolic free calcium in T cells , 1998, FEBS letters.

[18]  R. Levy,et al.  Essential Requirement of Cytosolic Phospholipase A2for Activation of the Phagocyte NADPH Oxidase* , 1998, The Journal of Biological Chemistry.

[19]  P. Webster,et al.  Homotypic fusion between aggregated lysosomes triggered by elevated [Ca2+]i in fibroblasts. , 1997, Journal of cell science.

[20]  R. Kramer,et al.  Structure, function and regulation of Ca2+‐sensitive cytosolic phospholipase A2 (cPLA2) , 1997, FEBS letters.

[21]  T. Takuma,et al.  Role of Ca2+-independent phospholipase A2 in exocytosis of amylase from parotid acinar cells. , 1997, Journal of biochemistry.

[22]  A. Mukherjee,et al.  Lysosomal membrane stabilization by α-tocopherol against the damaging action of Vipera russelli venom phospholipase A2 , 1997, Cellular and Molecular Life Sciences CMLS.

[23]  K. Emerk,et al.  17 beta-Estradiol increases intracellular free calcium concentrations of human vascular endothelial cells and modulates its responses to acetylcholine. , 1997, Endothelium : journal of endothelial cell research.

[24]  B. Dufy,et al.  17β-Estradiolid Ca influx rapid Ca2+ prostate cancer cells , 1996 .

[25]  R. Boland,et al.  Acute stimulation of intestinal cell calcium influx induced by 17β-estradiol via the cAMP messenger system , 1996, Molecular and Cellular Endocrinology.

[26]  M. Moore,et al.  Production of reactive oxygen species by hemocytes from the marine mussel, Mytilus edulis: lysosomal localization and effect of xenobiotics. , 1996, Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology.

[27]  P. Kuchel,et al.  Phospholipid composition of erythrocyte membranes and plasma of mammalian blood including Australian marsupials; quantitative 31P NMR analysis using detergent. , 1996, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[28]  R. Wattiaux,et al.  Lysosome pharmacology and toxicology. , 1996, Sub-cellular biochemistry.

[29]  J. Lloyd,et al.  Biology of the Lysosome , 1996, Subcellular Biochemistry.

[30]  William L. Smith,et al.  Calcium-mediated Translocation of Cytosolic Phospholipase A2 to the Nuclear Envelope and Endoplasmic Reticulum (*) , 1995, The Journal of Biological Chemistry.

[31]  M. Cajaraville,et al.  Morphofunctional study of the haemocytes of the bivalve mollusc Mytilus galloprovincialis with emphasis on the endolysosomal compartment. , 1995, Cell structure and function.

[32]  D. Harris,et al.  Mechanisms of iron-induced proximal tubule injury in rat remnant kidney. , 1995, The American journal of physiology.

[33]  D. Lowe,et al.  Contaminant induced lysosomal membrane damage in marine mussel digestive cells: an in vitro study , 1994 .

[34]  E. Brown,et al.  Inhibition of endosome fusion by phospholipase A2 (PLA2) inhibitors points to a role for PLA2 in endocytosis. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[35]  U. Brunk,et al.  H2O2-mediated damage to lysosomal membranes of J-774 cells. , 1993, Free radical research communications.

[36]  J. D. Clark,et al.  A novel arachidonic acid-selective cytosolic PLA2 contains a Ca2+-dependent translocation domain with homology to PKC and GAP , 1991, Cell.

[37]  A. Köhler,et al.  Lysosomal perturbations in fish liver as indicators for toxic effects of environmental pollution. , 1991, Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology.

[38]  J. Axelrod,et al.  Receptor-mediated activation of phospholipase A2 and arachidonic acid release in signal transduction. , 1990, Biochemical Society transactions.

[39]  M. Reis-Henriques,et al.  Variations in the levels of progesterone in Mytilus edulis during the annual reproductive cycle , 1990 .

[40]  J. Rémy-Martin,et al.  Studies of endogenous steroids from the marine mollusc Mytilus edulis L. By gas chromatography and mass spectrometry , 1990 .

[41]  N. Skakkebaek,et al.  Insulin-like growth factor I (somatomedin C) in goats during normal lactation and in response to somatotropin treatment. , 1990, Comparative biochemistry and physiology. A, Comparative physiology.

[42]  S. Orrenius,et al.  Role of Ca2+ in toxic cell killing. , 1989, Trends in pharmacological sciences.

[43]  A. Ciobanu,et al.  Histochemical investigation of myocardial proteases in heart anoxia, under protection with cardioplegic solution and protease inhibitors. , 1989, Physiologie.

[44]  Gevorkian Ga,et al.  Role of phospholipids in changes in lysosomal membrane stability in conditions of chronic alcoholic intoxication , 1985 .

[45]  M. Moore.  Cellular responses to pollutants , 1985 .

[46]  R. Tsien,et al.  A new generation of Ca2+ indicators with greatly improved fluorescence properties. , 1985, The Journal of biological chemistry.

[47]  G. Gevorkian,et al.  [Role of phospholipids in changes in lysosomal membrane stability in conditions of chronic alcoholic intoxication]. , 1985, Biulleten' eksperimental'noi biologii i meditsiny.

[48]  J. Widdows,et al.  Responses Of The Mussel Mytilus-Edulis To Copper And Phenanthrene - Interactive Effects , 1984 .

[49]  W. Weglicki,et al.  Temporal relationship of free radical-induced lipid peroxidation and loss of latent enzyme activity in highly enriched hepatic lysosomes. , 1983, The Journal of biological chemistry.

[50]  M. Condorelli,et al.  Possible role of arachidonic acid and of phospholipase A2 in the control of lysosomal enzyme release from human polymorphonuclear leukocytes. , 1983, Journal of clinical & laboratory immunology.

[51]  Szego Cm.  Lysosomal function in nucleocytoplasmic communication. , 1975 .

[52]  C. M. Szego.  Lysosomal function in nucleocytoplasmic communication. , 1975, Frontiers of biology.