The structure of a transcriptional unit on colicin E1 plasmid.

In an RNA-synthesizing system in vitro, a low-molecular-weight RNA consisting of about 110 residues (RNA-I) was efficiently synthesized on DNA of colicin E 1 plasmid (ColE1) and its deletion derivatives. The promoter site for RNA-I was analysed by testing the RNA polymerase-binding ability and template activity of restriction fragments; it was mapped in the region between the replication initiation site and the colicin immunity gene of ColE1. The direction of transcription was determined by hybridization tests to the separated strands of the template. The DNA region directing RNA-I was sequenced, and RNA-I was assigned on the sequence based on the nearest-neighbour data of RNA. The sequences of its promoter and terminator regions were also deduced. Although the function of this small RNA species is unknown, a unique secondary structure could be constructed from its sequence and sensitivity to RNase.

[1]  M. Calos,et al.  DNA sequence for a low-level promoter of the lac repressor gene and an ‘up’ promoter mutation , 1978, Nature.

[2]  J. Davies,et al.  Low molecular weight RNA species encoded by a multiple drug resistance plasmid. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[3]  C. Yanofsky,et al.  Comparison of the nucleotide sequences of the initial transcribed regions of the tryptophan operons of Escherichia coli and Salmonella typhimurium. , 1978, Journal of molecular biology.

[4]  A. Oka.  Fine cleavage map of a small colicin E1 plasmid carrying genes responsible for replication and colicin E1 immunity , 1978, Journal of bacteriology.

[5]  K. Shimada,et al.  Colicinogenic mutant of ColE1 plasmid that fails to confer immunity to colicin E1. , 1978, Gene.

[6]  H. Sugisaki.  Recognition sequence of a restriction endonuclease from Haemophilus gallinarum. , 1978, Gene.

[7]  F. Bolivar,et al.  Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9. , 1977, Gene.

[8]  J. Inselburg.  Studies of colicin E1 plasmid functions by analysis of deletions and TnA insertions of the plasmid , 1977, Journal of bacteriology.

[9]  C. Yanofsky,et al.  Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[10]  K. Sugimoto,et al.  DNA regions essential for the function of a bacteriophage fd promoter. , 1977, Nucleic Acids Research.

[11]  J. Tomizawa,et al.  Origin of replication of colicin E1 plasmid DNA. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[12]  P. Seeburg,et al.  Interaction of RNA polymerase with promoters from bacteriophage fd. , 1977, European journal of biochemistry.

[13]  W. Gilbert,et al.  A new method for sequencing DNA. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[14]  J. Inselburg.  Isolation, mapping, and examination of effects of TnA insertions in ColE1 plasmids , 1977, Journal of bacteriology.

[15]  G. Hobom,et al.  DNA base sequence of the po promoter region of phage λ , 1977, Nature.

[16]  M. Takanami,et al.  Cleavage map of colicin E1 plasmid , 1976, Nature.

[17]  D. Helinski,et al.  Characterization of a mini-ColC1 plasmid , 1976, Journal of bacteriology.

[18]  K. Sugimoto,et al.  Sequence of promoter for coat protein gene of bacteriophage fd , 1976, Nature.

[19]  M. Rosenberg,et al.  Determination of nucleotide sequences beyond the sites of transcriptional termination. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[20]  M. Ptashne,et al.  Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[21]  D. Pribnow,et al.  Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites. , 1975, Journal of molecular biology.

[22]  M. Ptashne,et al.  Lambda repressor turns off transcription of its own gene. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[23]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[24]  C. Yanofsky,et al.  New features of the regulation of the tryptophan operon. , 1975, Science.

[25]  K. Sugimoto,et al.  Studies on bacteriophage fd DNA. II. Localization of RNA initiation sites on the cleavage map of the fd genome. , 1975, Journal of molecular biology.

[26]  K. Sugimoto,et al.  Studies on bacteriophage fd DNA. IV. The sequence of messenger RNA for the major coat protein gene. , 1975, Journal of molecular biology.

[27]  Vincenzo Pirrotta,et al.  Sequence of the PR promoter of phage λ , 1975, Nature.

[28]  D. Pribnow.  Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[29]  P. Seeburg,et al.  Mapping and characterization of promoters in bacteriophages fd, f1 and m13. , 1975, Journal of molecular biology.

[30]  K. Sugimoto,et al.  The nucleotide sequence of an RNA polymerase binding site on bacteriophage fd DNA , 1975, Nature.

[31]  C. Gray,et al.  Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[32]  W. Reznikoff,et al.  Genetic regulation: the Lac control region. , 1975, Science.

[33]  T. Takeya,et al.  Isolation of DNA segments containing promoters from bacteriophage T3 DNA. , 1974, Biochemistry.

[34]  J. Tomizawa,et al.  Termination point of replication of colicin E1 plasmid DNA in cell extracts. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[35]  T. Ikemura,et al.  Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis. , 1973, The Journal of biological chemistry.

[36]  S. Heinemann,et al.  Bidirectional Transcription and the Regulation of Phage λ Repressor Synthesis , 1972 .

[37]  B. Barrell,et al.  Bacteriophage φ80-induced low molecular weight RNA , 1972 .

[38]  D. Clewell,et al.  Nature of Col E1 Plasmid Replication in Escherichia coli in the Presence of Chloramphenicol , 1972, Journal of bacteriology.

[39]  M. Sugiura,et al.  Termination of RNA transcription on the replicative form DNA of bacteriophage fd. , 1971, Journal of molecular biology.

[40]  S. Weissman,et al.  Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid. , 1971, The Journal of biological chemistry.

[41]  F. Sanger,et al.  Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose. , 1969, European journal of biochemistry.

[42]  D. Helinski,et al.  Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli. , 1968, Journal of molecular biology.

[43]  Dingman Cw,et al.  Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. , 1967 .

[44]  C. Richardson.  Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[45]  H. Boyer,et al.  Genetic and physical studies on the replication of ColE1-type plasmids. , 1979, Cold Spring Harbor symposia on quantitative biology.