New experimental animal model of intracardiac thrombus created with epicardial echocardiographic guidance.

BACKGROUND Models of intracardiac thrombus are very difficult to establish and have rarely been reported. We designed and established a new, inexpensive, practical animal model for intracardiac thrombus created with epicardial echocardiographic guidance. METHODS Male New Zealand white rabbits weighing 2 to 3.9 kg (3.10±0.58 kg) were used in this study. Cylindrical thrombi were created in plastic tubing and then aspirated with saline into a syringe. The thrombus in saline suspension was then slowly injected into a heart chamber and confirmed with echocardiography, including two-dimensional and contrast-enhanced ultrasound. RESULTS Intracardiac thrombi were created successfully in the left ventricle, right ventricle, and left and right atrial appendages. The average preparation time was about 3 hours. There were no significant differences among the four heart chambers in the success rate of thrombus model creation. Thrombi embolized to the pulmonary artery after failure of the right heart model. After failure of the left heart model, emboli were found in the carotid artery, renal artery, and truncus coeliacus. In two cases thrombi extended from the left ventricular apex into the aorta and in one case the thrombus extended from the left atrial appendage to the left atrium; there was no such extension from the other chambers. The rabbits' vital signs remained stable after establishment of the model, with no significant changes in heart structure or function. CONCLUSIONS This new method of creating an intracardiac thrombus model in rabbits showed initial success.

[1]  S. Gündüz,et al.  Brachial embolism from mechanical mitral valve thrombosis: multimodality imaging-guided successful thrombolysis. , 2018, Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis.

[2]  C. Bode,et al.  A molecular intravascular ultrasound contrast agent allows detection of activated platelets on the surface of symptomatic human plaques. , 2017, Atherosclerosis.

[3]  Zhenwei Yang,et al.  Rivaroxaban attenuates thrombosis by targeting the NF-κB signaling pathway in a rat model of deep venous thrombus , 2017, International journal of molecular medicine.

[4]  Xudong Wang,et al.  Gene Expression Profiling of Pulmonary Artery in a Rabbit Model of Pulmonary Thromboembolism , 2016, PloS one.

[5]  K. Peter,et al.  Thrombus-Targeted Theranostic Microbubbles: A New Technology towards Concurrent Rapid Ultrasound Diagnosis and Bleeding-free Fibrinolytic Treatment of Thrombosis , 2016, Theranostics.

[6]  Zhou Zhou,et al.  A novel model for evaluating thrombolytic therapy in dogs with ST-elevation myocardial infarction , 2016, BMC Cardiovascular Disorders.

[7]  Sabrina Z. Wang,et al.  Upregulation of canonical transient receptor potential channel in the pulmonary arterial smooth muscle of a chronic thromboembolic pulmonary hypertension rat model , 2015, Hypertension Research.

[8]  P. Pellikka,et al.  Contrast Echocardiography for Assessment of Left Ventricular Thrombi , 2014, Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine.

[9]  A. Almulhim,et al.  Left ventricular thrombus development during ventricular fibrillation and resolution during resuscitation in a swine model of sudden cardiac arrest. , 2014, Resuscitation.

[10]  Ching-Feng Cheng,et al.  Granulocyte-CSF induced inflammation-associated cardiac thrombosis in iron loading mouse heart and can be attenuated by statin therapy , 2011, Journal of Biomedical Science.

[11]  P. Khairy,et al.  Lower Incidence of Thrombus Formation With Cryoenergy Versus Radiofrequency Catheter Ablation , 2003, Circulation.

[12]  D. McPherson,et al.  Left Ventricular Thrombus Enhancement After Intravenous Injection of Echogenic Immunoliposomes: Studies in a New Experimental Model , 2002, Circulation.