Essential Oils of Tagetes minuta and Lavandula coronopifolia from Djibouti: Chemical Composition, Antibacterial Activity and Cytotoxic Activity against Various Human Cancer Cell Lines

The chemical composition of the essential oils of two plants (Tagetes minuta L. and Lavandula coronopifolia L.) harvested from the Day region (in the north of Djibouti) is the subject of this study. The extraction of essential oils was carried out by hydrodistillation, and the average yield was obtained at a rate of approximately 0.25% for Tagetes minuta L. and 0.42% for Lavandula coronopifolia L. The analyses of these essential oils by gas chromatography coupled with mass spectrometry identified 13 compounds in the essential oil of Tagetes minuta L., including dihydrotagetone (20.8%), artemisia (17.9%), (Z)-tagetenone (12.4%), (-)-spathulenol (11.0%) and estragole (9.5%), were obtained as majority compounds, with a percentage of 71.6%. The essential oil of Lavandula coronopifolia L. is characterized by the presence of 42 compounds, including cis-caryophyllene (18.9%), dehydronerolidol (12.8%), isolongifolanone (11.2%), caryophyllene oxide (8.2%), 10-epi-β-eudesmol (7.7%) and humulene (5.1%), were obtained as the majority chemical constituents, with a percentage of 63.9%. The antimicrobial activities of the essential oils at concentrations of 5% were measured against 12 bacterial strains (Gram positive: Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Streptococcus agalactiae (ATCC 27956), Staphylococcus epidermidis and Corynebacterium sp.; Gram Negative: Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603), Acinetobacter baumannii (ATCC 19606), Shigella sonnei (ATCC 9290), Salmonella enterica sv. Typhimurium (ATCC 13311) and Enterobacter cloacae), and the results of in vitro experiments showed inhibitory effects against most strains tested except Staphylococcus aureus, Enterococcus faecalis and Streptococcus agalactiae. Additionally, both oils were tested for their ability to selectively kill 13 human cancer cells (K562, A549, HCT116, PC3, U87-MG, MIA-Paca2, HEK293, NCI-N87, RT4, U2OS, A2780, MRC-5 and JIMT-T1), and the results obtained, according to the values of IC50, show the significant activity of two essential oils, particularly on the HCT116 and A2780 lines, which present values between 0.25 µg/mL and 0.45 µg/mL, respectively.

[1]  M. Emam,et al.  Green silver nanoparticles based on Lavandula coronopifolia aerial parts extract against mycotic mastitis in cattle , 2022, Biocatalysis and Agricultural Biotechnology.

[2]  M. Sharifi-Rad,et al.  Phytochemical characterization and assessments of antimicrobial, cytotoxic and anti-inflammatory properties of Lavandula coronopifolia Poir. volatile oil from Palestine , 2022, Arabian Journal of Chemistry.

[3]  E. Omara,et al.  Evaluation of the Anti-Cancer/Anti-Mutagenic Efficiency of Lavandula officinalis Essential Oil , 2022, Asian Pacific journal of cancer prevention : APJCP.

[4]  A. Baun,et al.  Separating toxicity and shading in algal growth inhibition tests of nanomaterials and colored substances , 2022, Nanotoxicology.

[5]  Rakshak Kumar,et al.  Seasonal variability in essential oil composition and biological activity of Rosmarinus officinalis L. accessions in the western Himalaya , 2022, Scientific Reports.

[6]  Yixin Zhang,et al.  Identification of novel non-toxic and anti-angiogenic α-fluorinated chalcones as potent colchicine binding site inhibitors , 2022, Journal of enzyme inhibition and medicinal chemistry.

[7]  T. Zair,et al.  Phytochemical Analysis, Antimicrobial and Antioxidant Properties of Thymus zygis L. and Thymus willdenowii Boiss. Essential Oils , 2021, Plants.

[8]  M. Assari,et al.  Sequential ultrasound-microwave technique as an efficient method for extraction of essential oil from Lavandula coronopifolia Poir , 2021, Journal of Food Measurement and Characterization.

[9]  R. Humphries,et al.  Overview of Changes to the Clinical and Laboratory Standards Institute Performance Standards for Antimicrobial Susceptibility Testing, M100, 31st Edition , 2021, Journal of clinical microbiology.

[10]  A. Barros,et al.  Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources , 2021, Scientific Reports.

[11]  Mozhdeh Zamani,et al.  In Vitro Cytotoxicity and Apoptotic Assay of Eucalyptus globulus Essential Oil in Colon and Liver Cancer Cell Lines , 2021, Journal of Gastrointestinal Cancer.

[12]  M. Sobeh,et al.  Phytochemical Profiling of Lavandula coronopifolia Poir. Aerial Parts Extract and Its Larvicidal, Antibacterial, and Antibiofilm Activity Against Pseudomonas aeruginosa , 2021, Molecules.

[13]  M. Matsabisa,et al.  Tagetes minuta leaf extracts triggered apoptosis in MCF-7 human breast cancer cell line , 2021 .

[14]  Tarik Ainane,et al.  Behaviour desorption study of the essential oil of Cedrus atlantica in a porous clay versus insecticidal activity against Sitophilus granarius: explanation of the phenomenon by statistical studies , 2021, International Journal of Metrology and Quality Engineering.

[15]  Tarik Ainane,et al.  CHEMICAL STUDY AND EVALUATION OF INSECTICAL PROPERTIES OF AFRICAN LIPPIA CITRIODORA ESSENTIAL OIL , 2020, Journal of Biopesticides.

[16]  Mohamed Ibourki,et al.  Chemical Characterization of The Essential Oil From Aerial Parts of Lavandula rejdalii Upson & Jury, a Medicinal Plant Endemic to Morocco , 2020, Journal of Essential Oil Bearing Plants.

[17]  Rakesh Kumar,et al.  Variability in chemical composition and antimicrobial activity of Tagetes minuta L. essential oil collected from different locations of Himalaya , 2020, Industrial Crops and Products.

[18]  Tarik Ainane.  Moroccan traditional treatment for fever and influenza, similar to symptoms of coronavirus COVID-19 disease: Mini Review , 2020 .

[19]  Adnane,et al.  CHEMICAL COMPOSITIONS, INSECTICIDAL AND ANTIMICROBIAL ACTIVITIES OF TWO MOROCCAN ESSENTIAL OILS OF CITRUS LIMONUM AND SYZYGIUM AROMATICUM , 2020 .

[20]  S. Charaf,et al.  Treatment of mycoses by essential oils: Mini Review , 2020 .

[21]  A. Romane,et al.  Antibacterial activity and chemical composition of essential oil from Lavandula tenuisecta Coss.ex Ball. an endemic species from Morocco , 2020 .

[22]  L. Pistelli,et al.  Chemical Composition and in Vitro Cytotoxic Screening of Sixteen Commercial Essential Oils on Five Cancer Cell Lines , 2019, Chemistry & biodiversity.

[23]  A. Mehta,et al.  Essential oils: from prevention to treatment of skin cancer. , 2019, Drug discovery today.

[24]  J. Wagacha,et al.  Chemical Composition and Antibacterial Activity of Essential Oils of Tagetes minuta (Asteraceae) against Selected Plant Pathogenic Bacteria , 2016, International journal of microbiology.

[25]  M. Talbi,et al.  Two natural compounds – a benzofuran and a phenylpropane – from Artemisia dracunculus , 2016, Journal of Asian natural products research.

[26]  J. Musarrat,et al.  Hepatoprotective potential of Lavandula coronopifolia extracts against ethanol induced oxidative stress-mediated cytotoxicity in HepG2 cells , 2015, Toxicology and industrial health.

[27]  L. Salgueiro,et al.  Chemical composition and antibacterial activity of Lavandula coronopifolia essential oil against antibiotic-resistant bacteria , 2015, Natural product research.

[28]  G. Maru,et al.  The role of inflammation in skin cancer. , 2014, Advances in experimental medicine and biology.

[29]  A. Gamal,et al.  The chemical composition and antimicrobial activity of the essential oil of Lavandula coronopifolia growing in Saudi Arabia , 2014 .

[30]  M. Boussaid,et al.  Chemical composition and antioxidant activities of essential oils and methanol extracts of three wild Lavandula L. species , 2012, Natural product research.

[31]  E. Chamorro,et al.  Chemical composition of essential oil from tagetes minuta L. leaves and flowers , 2008 .

[32]  D. K. Rajput,et al.  Comparative Chemical Composition of Steam-Distilled and Water-Soluble Essential Oils of South American Marigold (Tagetes minuta L.) , 2006 .

[33]  A. Pichette,et al.  Essential Oils from Bolivia. IV. Compositae: Tagetes aff. maxima Kuntze and Tagetes multiflora H.B.K. , 2005 .

[34]  F. Napolitano,et al.  Antibacterial activity of Tagetes minuta L. (Asteraceae) essential oil with different chemical composition , 2004 .

[35]  C. Soussy,et al.  Comité de l'Antibiogramme de la Société Française de Microbiologie report 2003. , 2003, International journal of antimicrobial agents.

[36]  J. Chalchat,et al.  Essential Oils of Four Wild and Semi-Wild Plants from Zimbabwe: Colospermum mopane (Kirk ex Benth.) Kirk ex Leonard, Helichrysum splendidum (Thunb.) Less, Myrothamnus flabellifolia (Welw.) and Tagetes minuta L. , 1999 .

[37]  G. R. Castro,et al.  Antimicrobial activity of flavonoids from leaves of Tagetes minuta. , 1997, Journal of ethnopharmacology.

[38]  J. Chalchat,et al.  Essential Oil of Tagetes minuta from Rwanda and France: Chemical Composition According to Harvesting Location, Growth Stage and Part of Plant Extracted , 1995 .

[39]  M. Green,et al.  Larvicidal activity of Tagetes minuta (marigold) toward Aedes aegypti. , 1991, Journal of the American Mosquito Control Association.

[40]  J. W. Alencar,et al.  Essential oils of Tagetes minuta from Brazil , 1988 .