Cytotoxicity of ophthalmic solutions with and without preservatives to human corneal endothelial cells, epithelial cells and conjunctival epithelial cells

Purpose:  The cytotoxicity of a range of commercial ophthalmic solutions in the presence and absence of preservatives was assessed in human corneal endothelial cells (HCECs), corneal epithelia and conjunctival epithelia using in vitro techniques.

[1]  M. Ayaki,et al.  Effect of ophthalmic solution components on acrylic intraocular lenses , 2007, Journal of cataract and refractive surgery.

[2]  C. Baudouin.  Allergic reaction to topical eyedrops. , 2005, Current opinion in allergy and clinical immunology.

[3]  R. Noecker,et al.  Corneal and Conjunctival Changes Caused by Commonly Used Glaucoma Medications , 2004, Cornea.

[4]  A. Vasavada,et al.  Corneal endothelial safety of intracameral preservative-free 1% xylocaine. , 2004, Indian journal of ophthalmology.

[5]  C. Creuzot-Garcher,et al.  Conjunctival proinflammatory and proapoptotic effects of latanoprost and preserved and unpreserved timolol: an ex vivo and in vitro study. , 2004, Investigative ophthalmology & visual science.

[6]  S. Kinoshita,et al.  Comparison of the Short-Term Effects on the Human Corneal Surface of Topical Timolol Maleate With and Without Benzalkonium Chloride , 2003, Journal of glaucoma.

[7]  Parikh Ch,et al.  Ocular surgical pharmacology: corneal endothelial safety and toxicity. , 2003 .

[8]  F. Raiskup,et al.  Inadvertent conjunctival trauma related to contact with drug container tips: a masquerade syndrome. , 2003, Ophthalmology.

[9]  P. McDonnell,et al.  Histopathology of corneal melting associated with diclofenac use after refractive surgery , 2003, Journal of cataract and refractive surgery.

[10]  A. Lambiase,et al.  Preservative-free diclofenac sodium 0.1% for vernal keratoconjunctivitis , 2002, Graefe's Archive for Clinical and Experimental Ophthalmology.

[11]  K. Teichmann,et al.  Toxic endothelial cell destruction from intraocular benzalkonium chloride , 2001, Journal of cataract and refractive surgery.

[12]  I. Udell,et al.  Keratitis, ulceration, and perforation associated with topical nonsteroidal anti-inflammatory drugs. , 2001, Ophthalmology.

[13]  F. Sauerburger,et al.  The role of matrix metalloproteinases in ulcerative keratolysis associated with perioperative diclofenac use. , 2001, Ophthalmology.

[14]  N. Joyce,et al.  EDTA: a promoter of proliferation in human corneal endothelium. , 2000, Investigative ophthalmology & visual science.

[15]  G. Feldmann,et al.  Effects of benzalkonium chloride on growth and survival of Chang conjunctival cells. , 1999, Investigative ophthalmology & visual science.

[16]  Y. Esumi,et al.  Ocular Tissue Distribution in Rabbit after Instillation of Bromfenac Sodium Ophthalmic Solution , 1999 .

[17]  O. Nishi,et al.  Effects of diclofenac sodium and indomethacin on proliferation and collagen synthesis of lens epithelial cells in vitro , 1995, Journal of cataract and refractive surgery.

[18]  M. Ishiyama,et al.  A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye , 1993 .

[19]  E. Pels,et al.  Toxic effects of detergents on the corneal endothelium. , 1990, Archives of ophthalmology.

[20]  L. Zimmerman,et al.  Toxic endothelial degeneration in ocular surface disease treated with topical medications containing benzalkonium chloride. , 1988, American journal of ophthalmology.

[21]  T. Mosmann.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.

[22]  R. Berkelman,et al.  Increased bactericidal activity of dilute preparations of povidone-iodine solutions , 1982, Journal of clinical microbiology.

[23]  N. Burstein.  Corneal cytotoxicity of topically applied drugs, vehicles and preservatives. , 1980, Survey of ophthalmology.

[24]  N. Burstein.  Preservative cytotoxic threshold for benzalkonium chloride and chlorhexidine digluconate in cat and rabbit corneas. , 1980, Investigative ophthalmology & visual science.

[25]  C. A. Stone,et al.  Comparison of the effects of timolol and other adrenergic agents on intraocular pressure in the rabbit. , 1977, Investigative ophthalmology & visual science.

[26]  G. Klintworth,et al.  The pathogenesis of corneal edema induced by Tween 80. , 1967, The American journal of pathology.

[27]  S. Hubbard.  Comparative toxicology of borates , 2007, Biological Trace Element Research.

[28]  H. Edelhauser,et al.  Ocular surgical pharmacology: corneal endothelial safety and toxicity. , 2003, Current opinion in ophthalmology.

[29]  I. Ota,et al.  Latanoprost accelerates disruption of the blood-aqueous barrier and the incidence of angiographic cystoid macular edema in early postoperative pseudophakias. , 1999, Archives of ophthalmology.

[30]  C. Baudouin,et al.  Toxicity of preserved and unpreserved antiglaucoma topical drugs in an in vitro model of conjunctival cells. , 1999, Current Eye Research.

[31]  P. Ellis,et al.  Intraocular diclofenac and flurbiprofen concentrations in human aqueous humor following topical application. , 1994, Journal of ocular pharmacology.

[32]  R. Tripathi,et al.  Cytotoxic effects of benzalkonium chloride and chlorobutanol on human corneal epithelial cells in vitro. , 1989, Lens and eye toxicity research.

[33]  M. Araie,et al.  Beta-adrenergic blockers: ocular penetration and binding to the uveal pigment. , 1982, Japanese journal of ophthalmology.