Toxicologic evaluation of bacterial synthesized cellulose in endothelial cells and animals

Compared to plant cellulose, bacterial cellulose synthesized by Gluconacetobacter xylinus has features such as high crystallinity, tensile strength and water absorption capacity; biocompatibility; resistance to degradation and low solubility that may be advantageous for engineered tissue. However, little information is available concerning the potential toxicity of bacterial cellulose-based biomaterials. The present study investigated the toxicity of bacterial cellulose nanofibers in vitro in human umbilical vein endothelial cells (HUVECs) using viability and flow cytometric assays and in vivo using C57/Bl6 mice. The absence of toxicity in vitro and in vivo supports the view that bacterial cellulose may be amenable for use as a tissue engineering biomaterial.

[1]  D. Updegraff.  Semimicro determination of cellulose in biological materials. , 1969, Analytical biochemistry.

[2]  Haosu Luo,et al.  Strain-mediated electric-field control of resistance in the La[sub 0.85]Sr[sub 0.15]MnO₃/0.7Pb(Mg[sub ⅓]Nb[sub ⅔])O₃-0.3PbTiO₃ structure , 2007 .

[3]  R. Brown,et al.  Microbial cellulose--the natural power to heal wounds. , 2006, Biomaterials.

[4]  B. Banfield,et al.  An integral membrane green fluorescent protein marker, Us9-GFP, is quantitatively retained in cells during propidium iodide-based cell cycle analysis by flow cytometry. , 1999, Experimental cell research.

[5]  Y. Sugano,et al.  Recent advances in bacterial cellulose production , 2005 .

[6]  Kunihiko Watanabe,et al.  Bacterial Cellulose ; as a New Material for Papermaking , 1995 .

[7]  M. Schramm,et al.  Synthesis of cellulose by Acetobacter xylinum. II. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. , 1954, The Biochemical journal.

[8]  T. Ulich,et al.  The intratracheal administration of endotoxin and cytokines. I. Characterization of LPS-induced IL-1 and TNF mRNA expression and the LPS-, IL-1-, and TNF-induced inflammatory infiltrate. , 1991, The American journal of pathology.

[9]  D. Schwartz,et al.  Relevance of mutations in the TLR4 receptor in patients with gram-negative septic shock. , 2002, Archives of internal medicine.

[10]  Regina Vasconcellos Antônio,et al.  Template assisted synthesis of porous nanofibrous cellulose membranes for tissue engineering , 2008 .

[11]  P. Mahadevan,et al.  An overview , 2007, Journal of Biosciences.

[12]  J. D. Fontana,et al.  Acetobacter cellulose pellicle as a temporary skin substitute , 1990, Applied biochemistry and biotechnology.

[13]  Jayoung Kim,et al.  Acrolein Induces Cyclooxygenase-2 and Prostaglandin Production in Human Umbilical Vein Endothelial Cells: Roles of p38 MAP Kinase , 2007, Arteriosclerosis, thrombosis, and vascular biology.

[14]  D. Kaplan,et al.  Bacterial cellulose as a potential scaffold for tissue engineering of cartilage. , 2005, Biomaterials.

[15]  F. M. Gama,et al.  BC nanofibres: in vitro study of genotoxicity and cell proliferation. , 2009, Toxicology letters.

[16]  P. Gatenholm,et al.  Intravital fluorescent microscopic evaluation of bacterial cellulose as scaffold for vascular grafts. , 2009, Journal of biomedical materials research. Part A.

[17]  R. Kaomongkolgit,et al.  Growth of Human Keratinocytes and Fibroblasts on Bacterial Cellulose Film , 2006, Biotechnology progress.

[18]  Masatoshi Iguchi,et al.  Bacterial cellulose—a masterpiece of nature's arts , 2000 .

[19]  S. Anderson,et al.  Biogenesis of bacterial cellulose. , 1991, Critical reviews in microbiology.

[20]  D. Remick,et al.  Intratracheal administration of endotoxin and cytokines. VII. The soluble interleukin-1 receptor and the soluble tumor necrosis factor receptor II (p80) inhibit acute inflammation. , 1994, Clinical immunology and immunopathology.

[21]  D. Danon,et al.  SYNTHESIS OF CELLULOSE BY ACETOBACTER XYLINUM V. Ultrastructure of Polymer , 1962 .

[22]  Xin Lu,et al.  E2F1-induced apoptosis requires DNA binding but not transactivation and is inhibited by the retinoblastoma protein through direct interaction. , 1997, Genes & development.

[23]  A. Zeiher,et al.  Apoptosis and Heart Failure: A Critical Review of the Literature Vascular Cell Apoptosis in Remodeling, Restenosis, and Plaque Rupture , 2022 .

[24]  Paul Gatenholm,et al.  Influence of cultivation conditions on mechanical and morphological properties of bacterial cellulose tubes , 2007, Biotechnology and bioengineering.

[25]  Y. Yamada,et al.  The phylogeny of acetic acid bacteria based on the partial sequences of 16S ribosomal RNA: the elevation of the subgenus Gluconoacetobacter to the generic level. , 1997, Bioscience, biotechnology, and biochemistry.

[26]  R. Sgonc,et al.  Apoptosis detection: an overview , 1998, Experimental Gerontology.

[27]  A. Basta,et al.  PRODUCTION AND CHARACTERIZATION OF ECONOMICAL BACTERIAL CELLULOSE , 2008 .

[28]  Huajian Gao,et al.  Ultrasonic technique for extracting nanofibers from nature materials , 2007 .

[29]  C Haanen,et al.  A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. , 1995, Journal of immunological methods.

[30]  Dieter Klemm,et al.  Bacterial synthesized cellulose — artificial blood vessels for microsurgery , 2001 .

[31]  J. W. Owens,et al.  The toxicity of purified cellulose in studies with laboratory animals. , 1992, Cancer letters.

[32]  R Mayer,et al.  Cellulose biosynthesis and function in bacteria. , 1991, Microbiological reviews.