Establishment of an immortalized laryngeal posterior commissure cell line as a tool for reflux research

Laryngopharyngeal reflux (LPR) has been implicated as a promoter of laryngeal cancer. Within the larynx, the posterior commissure (PC) is the region that usually comes into direct contact with refluxed materials. Specific laryngeal cell lines useful for in vitro studies are not widely available, and noncancer‐derived PC laryngeal cell line has not yet been described.

[1]  E. A. Kelly,et al.  Chronic Pepsin Exposure Promotes Anchorage-Independent Growth and Migration of a Hypopharyngeal Squamous Cell Line , 2014, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[2]  C. Silver,et al.  Relationship between reflux and laryngeal cancer , 2013, Head & neck.

[3]  G. Stoner,et al.  Pepsin promotes proliferation of laryngeal and pharyngeal epithelial cells , 2012, The Laryngoscope.

[4]  Seung Hwan Lee,et al.  The Role of Laryngopharyngeal Reflux as a Risk Factor in Laryngeal Cancer: A Preliminary Report , 2011, Clinical and experimental otorhinolaryngology.

[5]  S. T. Tucker,et al.  Posterior commissure of the human larynx revisited. , 2010, Journal of voice : official journal of the Voice Foundation.

[6]  Tina L Samuels,et al.  Pepsin as a causal agent of inflammation during nonacidic reflux , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[7]  T. Shpitzer,et al.  T1 glottic carcinoma involving the posterior commissure , 2009, The Laryngoscope.

[8]  M. Birchall,et al.  The isolation and characterisation of primary human laryngeal epithelial cells. , 2005, Molecular immunology.

[9]  F. Paulsen,et al.  Functional and clinical anatomy of the posterior insertion of the human vocal ligament , 2006, European Archives of Oto-Rhino-Laryngology.

[10]  C. Ford.  Evaluation and management of laryngopharyngeal reflux. , 2005, JAMA.

[11]  A. Franchi,et al.  Epidermal growth factor receptor and p53 expression in T1–T2 glottic cancer involving the anterior or posterior commissure , 2004, Acta oto-laryngologica.

[12]  P. Sacks.  Cell, tissue and organ culture as in vitro models to study the biology of squamous cell carcinomas of the head and neck , 1996, Cancer and Metastasis Reviews.

[13]  C. Pellegrini,et al.  Histologic features of reflux-attributed laryngeal lesions. , 2003, The American journal of medicine.

[14]  P. Romagnoli,et al.  Risk Factors and Prognosis of Anterior Commissure versus Posterior Commissure T1-T2 Glottic Cancer , 2003, The Annals of otology, rhinology, and laryngology.

[15]  J. Koufman,et al.  Validity and reliability of the reflux symptom index (RSI). , 2002, Journal of voice : official journal of the Voice Foundation.

[16]  J. Koufman,et al.  The Validity and Reliability of the Reflux Finding Score (RFS) , 2001, The Laryngoscope.

[17]  T. Suzuki,et al.  Spontaneous mutation of p53 gene in human papillomavirus type 16 E7-immortalized human laryngeal epithelial cells. , 1996, Acta oto-laryngologica. Supplementum.

[18]  J. Koufman.  The Otolaryngologic Manifestations of Gastroesophageal Reflux Disease (GERD): A Clinical Investigation of 225 Patients Using Ambulatory 24‐Hour pH Monitoring and an Experimental Investigation of the Role of Acid and Pepsin in the Development of Laryngeal Injury , 1991, The Laryngoscope.

[19]  D. Thomas,et al.  Differences in the sex ratio of laryngeal cancer incidence rates by anatomic subsite. , 1989, Journal of clinical epidemiology.

[20]  Z. Radšel,et al.  Acid posterior laryngitis , 1984, The Journal of Laryngology & Otology.