论文信息 - Method for the decellularization of intact rat liver

Method for the decellularization of intact rat liver

We have developed a method for the decellularization of whole rat livers by perfusion with increasing concentrations of detergents. This procedure resulted in an intact, decellularized organ with an intact liver capsule. These decellularized organs were analyzed by immunohistochemistry, and retained an appropriate distribution of extracellular matrix components. The laminin basement membranes of the liver vasculature also remain intact. These acellular vessel remnants were strong enough to be cannulated; providing a convenient means for the delivery of cells to areas deep within the decellularized organ. Cannulation of the extrahepatic vessel remnants allow for media to be circulated through the decellularized organ. These decellularized livers provide a natural matrix for research in the fields of bio-artificial livers and liver engineering.

[1] M. Gómez-Lechón,et al. Long‐term expression of differentiated functions in hepatocytes cultured in three‐dimensional collagen matrix , 1998, Journal of cellular physiology.

[2] W. Mars,et al. Bone marrow as a potential source of hepatic oval cells. , 1999, Science.

[3] G. Michalopoulos,et al. Morphogenetic events in mixed cultures of rat hepatocytes and nonparenchymal cells maintained in biological matrices in the presence of hepatocyte growth factor and epidermal growth factor , 1999, Hepatology.

[4] K. Shakesheff,et al. Long-term culture of functional liver tissue: three-dimensional coculture of primary hepatocytes and stellate cells. , 2003, Tissue engineering.

[5] Peter Neuhaus,et al. Time course of primary liver cell reorganization in three-dimensional high-density bioreactors for extracorporeal liver support: an immunohistochemical and ultrastructural study. , 2004, Tissue engineering.

[6] Jan Hansmann,et al. Engineered liver-like tissue on a capillarized matrix for applied research. , 2007, Tissue engineering.

[7] Paul L. Martin,et al. Pretransplant management of the cirrhotic patient. , 2007, Clinics in liver disease.

[8] A. Gasbarrini,et al. Isolation and characterization of hepatic stem cells, or "oval cells," from rat livers. , 2009, Methods in molecular biology.