The Use of Ciprofloxacin for Decontamination of LSCC-H 32 Cell Line of Chicken Embryonal Fibroblasts Contaminated by Mycoplasma arginini

Fischer 0., Marie Hajkova, Zuzana Hoi'inova, Kvetoslava Jurma­ nova, Dagmar Zendulkova: The Use of Ciprofloxacin for Decontamination of LSCC-H 32 Cell Line of Chicken Embryona[ Fibroblasts Contaminated by Mycoplasma arginini. Acta vet. Bmo, 61, 1992: 51-56. Cells of line LSCC-N 32 of chicken embryonal fibroblasts contaminated by My­ coplasma arginini were decontaminated by culture in a medium supplemented with 10 ILg per m1 of ciprofloxacin during 10 passages (42 days). During another 30 passa­ ges (112 days) in an antibiotic-free medium no mycoplasmas were detected in repea­ ted examination by the fluorescence method, culture in a liquid medium supple­ mented with glucose, urea or arginine, culture in a solid medium or by scanning electron microscopy. Decontamination by ciprofloxacin neither changed morpholo­ gical properties of cells nor increased number of aberrant mitoses. The LSCC-H 32 cells, decontaminated by ciprofloxacin, showed on day 5 of culture by 51.2 % higher proliferation index compared with cells of the same line contaminated by M. arginini. Proliferation index, the flnorescence method, mitosis