Partial sleep deprivation compromises gastric mucosal integrity in rats.

The gastric mucosa is most susceptible to stress that has been shown to induce mucosal damage in humans and animals. This study aims to explore the underlying mechanisms of partial sleep deprivation, as a source of psychophysiological stress, on gastric functions and its effect on mucosal integrity. Sprague-Dawley rats were partially sleep deprived (PSD) for 7 or 14 days by housing inside slowly rotating drums. Gastric tissues and plasma were sampled at the end of the sleep deprivation periods and mucosal lesion scores were evaluated. Morphological examination was performed after Hematoxylin and Eosin staining. Plasma levels of noradrenaline, adrenaline, gastrin, histamine and somatostatin were determined with enzyme immunoassays. Gastric acidity was measured with acid-base titration in pylorus ligated rats. Gastric mucosal blood flow was evaluated with Laser Doppler Flowmetry. It was found that gastric lesions were induced in about 30%-50% of the PSD rats. Gastric acidity as well as plasma levels of noradrenaline, gastrin and histamine were elevated. Gastric mucosal blood flow and plasma somatostatin level were on the contrary reduced, especially in rats with PSD for 14 days. It is concluded that partial sleep deprivation compromises gastric mucosal integrity by increasing gastric acidity, plasma levels of noradrenaline, gastrin, histamine, and decreasing gastric mucosal blood flow. These results provided experimental evidence on the gastric damaging effects of PSD and it could be one of the risk factors contributing to gastric ulcer formation.

[1]  C. Cho,et al.  Luminal acid in stress ulceration and the antiulcer action of verapamil in rat stomachs , 1986, The Journal of pharmacy and pharmacology.

[2]  D I Tepas,et al.  Sleep patterns of shiftworkers. , 1990, Occupational medicine.

[3]  R. Bresalier The Clinical Significance and Pathophysiology of Stress‐Related Gastric Mucosal Hemorrhage , 1991, Journal of clinical gastroenterology.

[4]  A. Rechtschaffen,et al.  Sleep deprivation in the rat: III. Total sleep deprivation. , 1989, Sleep.

[5]  S. Levenstein Stress and peptic ulcer: life beyond helicobacter , 1998, BMJ.

[6]  L. Toth,et al.  Systemic bacterial invasion induced by sleep deprivation. , 2000, American journal of physiology. Regulatory, integrative and comparative physiology.

[7]  C. Cho,et al.  Assessment of hemodynamic changes in rat stomachs by laser Doppler velocimetry and reflectance spectrophotometry. Effects of ethanol and prostaglandin E2 under ischemic and congestive conditions. , 1994, Digestion.

[8]  R. Durham,et al.  Stress gastritis revisited. , 1991, The Surgical clinics of North America.

[9]  C. Everson Sustained sleep deprivation impairs host defense. , 1993, The American journal of physiology.

[10]  L. Larsson Developmental biology of gastrin and somatostatin cells in the antropyloric mucosa of the stomach , 2000, Microscopy research and technique.

[11]  K. Ehrlich,et al.  Role of ATP-sensitive potassium channels in prostaglandin-mediated gastroprotection in the rat. , 2002, The Journal of pharmacology and experimental therapeutics.

[12]  P. Penev,et al.  High prevalence of sleep disturbance in cirrhosis , 1998, Hepatology.

[13]  G. Sachs,et al.  Acid suppression: optimizing therapy for gastroduodenal ulcer healing, gastroesophageal reflux disease, and stress-related erosive syndrome. , 2000, Gastroenterology.

[14]  E. Lindström,et al.  Neurohormonal regulation of secretion from isolated rat stomach ECL cells: a critical reappraisal , 2001, Regulatory Peptides.

[15]  E. Lindström,et al.  Rat stomach ECL cells up-date of biology and physiology. , 1999, General pharmacology.

[16]  J. Gruzelier,et al.  Adverse changes in mood and cognitive performance of house officers after night duty. , 1989, BMJ.

[17]  R. Caldara,et al.  Effect of L-dopa with and without inhibition of extra cerebral dopa decarboxylase on gastric acid secretion and gastrin release in man. , 1985, Gut.

[18]  J. Steingrub,et al.  Stress ulcer: is routine prophylaxis necessary? , 1995, The American journal of gastroenterology.

[19]  E. Hahn,et al.  Role of gastric acid secretion in progression of acute gastric erosions induced by ischemia-reperfusion into gastric ulcers. , 2000, European journal of pharmacology.

[20]  C. Cho,et al.  Over‐expression of inducible heat shock protein 70 in the gastric mucosa of partially sleep‐deprived rats , 2004, Scandinavian journal of gastroenterology.

[21]  R. De Giorgio,et al.  Effects of acute cold pressor test on vagally stimulated gastric acid secretion and circulating levels of human pancreatic polypeptide and gastrin. , 1994, Digestion.

[22]  H. Y. Chen,et al.  Fatal familial insomnia, a prion disease with a mutation at codon 178 of the prion protein gene. , 1992, The New England journal of medicine.

[23]  M. Respondek,et al.  Tachykinin-induced increase in gastric mucosal resistance: role of primary afferent neurons, CGRP, and NO. , 1996, The American journal of physiology.

[24]  H. Ursin,et al.  Sleep deprivation procedure produces stomach lesions in rats , 1982, Physiology & Behavior.

[25]  S. Levenstein The Very Model of a Modern Etiology: A Biopsychosocial View of Peptic Ulcer , 2000, Psychosomatic medicine.

[26]  T. Wehr,et al.  Nutritional and metabolic adaptations to prolonged sleep deprivation in the rat. , 1993, The American journal of physiology.

[27]  C. Cho,et al.  Modulatory action of adenosine on gastric function and ethanol-induced mucosal damage in rats , 1990, Digestive Diseases and Sciences.

[28]  Gene expression profiles in gastric mucosa of sleep deprivation rats. , 2000, World journal of gastroenterology.

[29]  C. Everson Functional consequences of sustained sleep deprivation in the rat , 1995, Behavioural Brain Research.