Use of peptide capture agents in porous silicon biosensors

We report the use of a peptide-based capture agent as an alternative to antibodies and nucleic acid-based bioreceptors for porous silicon biosensors. Click chemistry is employed to attach azide-functionalized streptavidin-binding peptides to alkyne-modified porous silicon films. The attachment of the streptavidin-binding peptide and subsequent detection of streptavidin molecules are verified using optical reflectance and Fourier transform infrared spectroscopy measurements.

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