Microscopy imaging and quantitative phase contrast mapping in turbid microfluidic channels by digital holography.

We show that sharp imaging and quantitative phase-contrast microcopy is possible in microfluidics in flowing turbid media by digital holography. In fact, in flowing liquids with suspended colloidal particles, clear vision is hindered and cannot be recovered by any other microscopic imaging technique. On the contrary, using digital holography, clear imaging is possible thanks to the Doppler frequency shift experienced by the photons scattered by the flowing colloidal particles, which do not contribute to the interference process, i.e. the recorded hologram. The method is illustrated and imaging results are demonstrated for pure phase objects, i.e. biological cells in microfluidic channels.

[1]  Karl A. Stetson,et al.  Holographic Fog Penetration , 1967 .

[2]  A. W. Lohmann,et al.  Image holography through convective fog , 1973 .

[3]  A. Lohmann,et al.  Holography through fog. A new version , 1978 .

[4]  G. Indebetouw.  Interferometric imaging through fog with a Fizeau interferometer , 1981 .

[5]  B. J. Chang,et al.  Imaging through scattering media by interferometric techniques. , 1981, Applied optics.

[6]  Marco Rito-Palomares,et al.  Dielectrophoresis for the manipulation of nanobioparticles , 2007, Electrophoresis.

[7]  P. Ferraro,et al.  Super-resolution in digital holography by a two-dimensional dynamic phase grating. , 2008, Optics express.

[8]  Chun-Hao Chen,et al.  Microfluidics and photonics for Bio‐System‐on‐a‐Chip: A review of advancements in technology towards a microfluidic flow cytometry chip , 2008, Journal of biophotonics.

[9]  Jeffrey S Erickson,et al.  The good, the bad, and the tiny: a review of microflow cytometry , 2008, Analytical and bioanalytical chemistry.

[10]  Zeev Zalevsky,et al.  Superresolution digital holographic microscopy for three-dimensional samples. , 2008, Optics express.

[11]  D. Kohlheyer,et al.  Miniaturizing free‐flow electrophoresis – a critical review , 2008, Electrophoresis.

[12]  Myung K. Kim,et al.  Digital holographic microscopy for quantitative cell dynamic evaluation during laser microsurgery. , 2009, Optics express.

[13]  R. Pethig.  Review article-dielectrophoresis: status of the theory, technology, and applications. , 2010, Biomicrofluidics.

[14]  Kishan Dholakia,et al.  Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation , 2010, Biomedical optics express.

[15]  Derek Tseng,et al.  Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications. , 2010, Lab on a chip.

[16]  Hongying Zhu,et al.  Holographic opto-fluidic microscopy , 2010, Optics express.

[17]  A Finizio,et al.  Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction. , 2010, Optics letters.

[18]  A Finizio,et al.  Driving and analysis of micro-objects by digital holographic microscope in microfluidics. , 2011, Optics letters.

[19]  Aydogan Ozcan,et al.  Field-portable lensfree tomographic microscope. , 2011, Lab on a Chip.

[20]  Wolfgang Osten,et al.  High-resolution quantitative phase microscopic imaging in deep UV with phase retrieval. , 2011, Optics letters.

[21]  Pietro Ferraro,et al.  Dynamic DIC by digital holography microscopy for enhancing phase-contrast visualization , 2011, Biomedical optics express.

[22]  B Javidi,et al.  Automatic focusing in digital holography and its application to stretched holograms. , 2011, Optics letters.

[23]  Demetri Psaltis,et al.  Three-dimensional scanning microscopy through thin turbid media. , 2012, Optics express.