Time-resolved fluorescence anisotropy studies of mixed lipid bilayers

Time-resolved fluorescence anisotropy of diphenylhexatriene (DPH) in various binary lipid mixtures of dimysterolphosphatidycholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), distearoylphosphatidylcholine (DSPC) and dioleoylphosphatidylcholine (DOPC) has been studied. Evidence is given for phase separation in binary mixtures of DMPC/DSPC and DOPC/DSPC which is not found in DMPC/DPPC and DPPC/DSPC mixtures. A rising anisotropy decay has been observed for DPH in binary lipid mixtures containing DSPC and in DSPC liposomes at the phase transition which suggests a heterogeneous environment for the fluorophore at this temperature.

[1]  R D Klausner,et al.  The concept of lipid domains in membranes , 1982, The Journal of cell biology.

[2]  M. Ameloot,et al.  Effect of orientational order on the decay of the fluorescence anisotropy in membrane suspensions. A new approximate solution of the rotational diffusion equation. , 1984, Biophysical journal.

[3]  E. Gratton,et al.  Detection of phospholipid phase separation. A multifrequency phase fluorimetry study of 1,6-diphenyl-1,3,5-hexatriene fluorescence. , 1984, The Journal of biological chemistry.

[4]  B. Lentz,et al.  Membrane “fluidity” as detected by diphenylhexatriene probes , 1989 .

[5]  R. Cundall,et al.  Fluorescence lifetime and quenching studies on some interesting diphenylhexatriene membrane probes , 1983 .

[6]  T. E. Thompson,et al.  Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 2 Two-component phosphatidylcholine liposomes. , 1976, Biochemistry.

[7]  Enrico Gratton,et al.  Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene in phospholipid vesicles. , 1987, Biochemistry.

[8]  R. Klausner,et al.  LIPID DOMAINS IN MEMBRANES * , 1982, Annals of the New York Academy of Sciences.

[9]  R. Dale,et al.  Nanosecond time-dependent fluorescence depolarization of diphenylhexatriene in dimyristoyllecithin vesicles and the determination of "microviscosity". , 1977, The Journal of biological chemistry.

[10]  R. Ludescher,et al.  Time-resolved fluorescence anisotropy for systems with lifetime and dynamic heterogeneity. , 1987, Biophysical chemistry.

[11]  K. Kinosita,et al.  Dynamic structure of lipid bilayers studied by nanosecond fluorescence techniques. , 1977, Biochemistry.