Patterns of molecular genetic variation among African elephant populations

The highly threatened African elephants have recently been subdivided into two species, Loxodonta africana (savannah or bush elephant) and L. cyclotis (forest elephant) based on morphological and molecular studies. A molecular genetic assessment of 16 microsatellite loci across 20 populations (189 individuals) affirms species level genetic differentiation and provides robust genotypic assessment of species affiliation. Savannah elephant popula‐tions show modest levels of phylogeographic subdivision based on composite microsatellite genotype, an indication of recent population isolation and restricted gene flow between locales. The savannah elephants show significantly lower genetic diversity than forest elephants, probably reflecting a founder effect in the recent history of the savannah species.

[1]  Ernst Mayr,et al.  Principles of systematic zoology , 1969 .

[2]  M Slatkin,et al.  A measure of population subdivision based on microsatellite allele frequencies. , 1995, Genetics.

[3]  H. Ellegren,et al.  Microsatellite ‘evolution’: directionality or bias? , 1995, Nature Genetics.

[4]  A. Templeton,et al.  Structure and history of African elephant populations: I. Eastern and southern Africa. , 1994, The Journal of heredity.

[5]  G. Craig,et al.  The African Elephant Database , 2001, Oryx.

[6]  N. Georgiadis,et al.  Genetic Evidence for Two Species of Elephant in Africa , 2001, Science.

[7]  F. Smith,et al.  A Remote Method for Obtaining Skin Biopsy Samples , 1987 .

[8]  J. Leonard,et al.  The Vegetation of Africa , 1984 .

[9]  P. Arctander,et al.  Isolation and characterization of microsatellite loci in the African elephant, Loxodonta africana. , 1998, Molecular ecology.

[10]  C. Groves,et al.  Living African elephants belong to two species: Loxodonta africana (Blumenbach, 1797) and Loxodonta cyclotis (Matschie, 1900) , 2000 .

[11]  L. Excoffier,et al.  Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. , 1992, Genetics.

[12]  N. Barton,et al.  Adaptation, speciation and hybrid zones , 1989, Nature.

[13]  S. Goodman,et al.  RST Calc: a collection of computer programs for calculating estimates of genetic differentiation from microsatellite data and determining their significance , 1997 .

[14]  W. Leuthold,et al.  Movements and patterns of habitat utilization of elephants in Tsavo National Park, Kenya , 1973 .

[15]  S. O’Brien,et al.  Bureaucratic mischief: recognizing endangered species and subspecies. , 1991, Science.

[16]  L. Tangley.  In Search of Africa's Forgotten Forest Elephant , 1997, Science.

[17]  Roderic D. M. Page,et al.  TreeView: an application to display phylogenetic trees on personal computers , 1996, Comput. Appl. Biosci..

[18]  B. Girdler-brown,et al.  Eastern and Southern Africa. , 1998, International migration.

[19]  V. J. Maglio.  Origin and evolution of the Elephantidae , 1973 .

[20]  Michael A. Banks,et al.  WHICHRUN (version 3.2): a computer program for population assignment of individuals based on multilocus genotype data. , 2000, The Journal of heredity.

[21]  L. J. Anthony.  The New Encyclopedia of Mammals , 2002 .

[22]  C. Groves,et al.  Do Loxodonta cyclotis and L. africana interbreed , 2000 .

[23]  B S Weir,et al.  Estimation of the coancestry coefficient: basis for a short-term genetic distance. , 1983, Genetics.

[24]  V. Barriel,et al.  Molecular phylogeny of Elephantidae. Extreme divergence of the extant forest African elephant. , 1999, Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie.

[25]  C. Thouless,et al.  African elephant database 1998 , 1995 .

[26]  E. Ostrander,et al.  Polymorphic microsatellite DNA loci identified in the African elephant (Loxodonta africana) , 2000, Molecular ecology.

[27]  L. Cavalli-Sforza,et al.  High resolution of human evolutionary trees with polymorphic microsatellites , 1994, Nature.