Evaluation of EPA's rapid bioassessment benthic metrics: Metric redundancy and variability among reference stream sites

The data analysis scheme used in the U.S. Environmental Protection Agency's (EPA's) rapid bioassessment protocols (RBPs) integrates several community, population, and functional parameters (or metrics) into a single assessment of biological condition. A reference data base of macroinvertebrate data obtained from 10 ecoregions in Oregon, Colorado, and Kentucky was used to evaluate the appropriateness and variability of the benthic metrics and the similarities of results among ecoregions. Several statistical procedures, including principal component analysis, correlation coefficient, analysis of variance, and stepwise discriminant analysis, were used to test the efficacy of 17 community metrics. A general separation between the mountain ecoregions and the valley/plains ecoregions was determined to exist for the metrics. Two of the original eight metrics described in the EPA's RBPs for benthic macroinvertebrates were found to be highly variable and unreliable as measures of biological conditions in some ecoregions. Eleven metrics were determined as being valuable in discriminating between montane and valley/plains groupings of ecoregions.