SUMO-specific proteases: a twist in the tail.

The small ubiquitin-like modifier (SUMO) is involved in many cellular processes and is required for normal growth and development in all eukaryotes. Whereas lower eukaryotes have a single version of SUMO, higher eukaryotes have three versions: SUMO-1, -2 and -3. Similarly to most other ubiquitin-like proteins, the primary translation products of the SUMO genes need to be proteolytically processed to expose the C-terminal glycine that will be linked to lysine side chains in substrates. Processing of SUMO precursors is mediated by SUMO-specific proteases that also remove SUMO from modified proteins and depolymerise poly-SUMO chains.

[1]  Luming Yin,et al.  Identification and Characterization of DEN1, a Deneddylase of the ULP Family* , 2003, Journal of Biological Chemistry.

[2]  N. Wei,et al.  DEN1 Is a Dual Function Protease Capable of Processing the C Terminus of Nedd8 and Deconjugating Hyper-neddylated CUL1* , 2003, Journal of Biological Chemistry.

[3]  Michael R. Kuehn,et al.  Mutation of SENP1/SuPr-2 Reveals an Essential Role for Desumoylation in Mouse Development , 2005, Molecular and Cellular Biology.

[4]  Min Wang,et al.  The Small Ubiquitin-like Modifier-1 (SUMO-1) Consensus Sequence Mediates Ubc9 Binding and Is Essential for SUMO-1 Modification* , 2001, The Journal of Biological Chemistry.

[5]  F. Z. Watts,et al.  Cell-cycle-dependent localisation of Ulp1, a Schizosaccharomyces pombe Pmt3 (SUMO)-specific protease. , 2002, Journal of cell science.

[6]  James H Naismith,et al.  The structure of SENP1-SUMO-2 complex suggests a structural basis for discrimination between SUMO paralogues during processing. , 2006, The Biochemical journal.

[7]  David Reverter,et al.  A basis for SUMO protease specificity provided by analysis of human Senp2 and a Senp2-SUMO complex. , 2004, Structure.

[8]  H. Su,et al.  Molecular features of human ubiquitin-like SUMO genes and their encoded proteins. , 2002, Gene.

[9]  M. Hochstrasser,et al.  The Ulp1 SUMO isopeptidase , 2003, The Journal of cell biology.

[10]  M. Dasso,et al.  Association of the Human SUMO-1 Protease SENP2 with the Nuclear Pore* , 2002, The Journal of Biological Chemistry.

[11]  Matthias Mann,et al.  A Proteomic Study of SUMO-2 Target Proteins* , 2004, Journal of Biological Chemistry.

[12]  H. Ploegh,et al.  The SUMO-Specific Protease SENP5 Is Required for Cell Division , 2006, Molecular and Cellular Biology.

[13]  Yongsok Kim,et al.  Desumoylation of homeodomain‐interacting protein kinase 2 (HIPK2) through the cytoplasmic‐nuclear shuttling of the SUMO‐specific protease SENP1 , 2005, FEBS letters.

[14]  C. Lima,et al.  Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast. , 2000, Molecular cell.

[15]  M. Tatham,et al.  A fluorescence-resonance-energy-transfer-based protease activity assay and its use to monitor paralog-specific small ubiquitin-like modifier processing. , 2007, Analytical biochemistry.

[16]  M. Hochstrasser,et al.  The Yeast ULP2 (SMT4) Gene Encodes a Novel Protease Specific for the Ubiquitin-Like Smt3 Protein , 2000, Molecular and Cellular Biology.

[17]  David Reverter,et al.  Structural basis for SENP2 protease interactions with SUMO precursors and conjugated substrates , 2006, Nature Structural &Molecular Biology.

[18]  M. Hochstrasser,et al.  A new protease required for cell-cycle progression in yeast , 1999, Nature.

[19]  L. Zon,et al.  SUMO-1 modification represses Sp3 transcriptional activation and modulates its subnuclear localization. , 2002, Molecular cell.

[20]  F. Melchior,et al.  SUMO: ligases, isopeptidases and nuclear pores. , 2003, Trends in biochemical sciences.

[21]  E. Yeh,et al.  Nucleocytoplasmic Shuttling Modulates Activity and Ubiquitination-Dependent Turnover of SUMO-Specific Protease 2 , 2006, Molecular and Cellular Biology.

[22]  G. Gill,et al.  Something about SUMO inhibits transcription. , 2005, Current opinion in genetics & development.

[23]  E. Yeh,et al.  Characterization of a Family of Nucleolar SUMO-specific Proteases with Preference for SUMO-2 or SUMO-3* , 2006, Journal of Biological Chemistry.

[24]  E. Yeh,et al.  Ubiquitin-like proteins: new wines in new bottles. , 2000, Gene.

[25]  G. Blobel,et al.  Mlp-dependent anchorage and stabilization of a desumoylating enzyme is required to prevent clonal lethality , 2004, The Journal of cell biology.

[26]  E. Koonin,et al.  Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulation. , 2001, Genetics.

[27]  B. Schulman,et al.  Breaking up with a kinky SUMO , 2006, Nature Structural &Molecular Biology.

[28]  Keith D Wilkinson,et al.  Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1. , 2005, Journal of molecular biology.

[29]  James H Naismith,et al.  Structural basis of NEDD8 ubiquitin discrimination by the deNEDDylating enzyme NEDP1 , 2005, The EMBO journal.

[30]  K. Wilkinson,et al.  SUSP1 antagonizes formation of highly SUMO2/3-conjugated species , 2006, The Journal of cell biology.

[31]  N. Perkins,et al.  P300 transcriptional repression is mediated by SUMO modification. , 2003, Molecular cell.

[32]  L. Liu,et al.  SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[33]  K. Bohren,et al.  A M55V Polymorphism in a Novel SUMO Gene (SUMO-4) Differentially Activates Heat Shock Transcription Factors and Is Associated with Susceptibility to Type I Diabetes Mellitus* , 2004, Journal of Biological Chemistry.

[34]  Tao Wang,et al.  Sumoylation of heterogeneous nuclear ribonucleoproteins, zinc finger proteins, and nuclear pore complex proteins: a proteomic analysis. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[35]  Ho Yin Chan,et al.  Crystal structure of the SENP1 mutant C603S-SUMO complex reveals the hydrolytic mechanism of SUMO-specific protease. , 2006, The Biochemical journal.

[36]  Y. Takahashi,et al.  Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins. , 2000, Journal of biochemistry.

[37]  S. H. Baek,et al.  A New SUMO-1-specific Protease, SUSP1, That Is Highly Expressed in Reproductive Organs* , 2000, The Journal of Biological Chemistry.

[38]  Hirofumi Tanaka,et al.  A novel mammalian Smt3-specific isopeptidase 1 (SMT3IP1) localized in the nucleolus at interphase. , 2000, European journal of biochemistry.

[39]  E. J. Lee,et al.  SUMO-specific protease SUSP4 positively regulates p53 by promoting Mdm2 self-ubiquitination , 2006, Nature Cell Biology.

[40]  H. Saitoh,et al.  Enzymes of the SUMO Modification Pathway Localize to Filaments of the Nuclear Pore Complex , 2002, Molecular and Cellular Biology.

[41]  Chia-Hui Yeh,et al.  SUMO modifications control assembly of synaptonemal complex and polycomplex in meiosis of Saccharomyces cerevisiae. , 2006, Genes & development.

[42]  V. Guacci,et al.  Pds5p regulates the maintenance of sister chromatid cohesion and is sumoylated to promote the dissolution of cohesion , 2003, The Journal of cell biology.

[43]  T. Asahara,et al.  Desumoylation Activity of Axam, a Novel Axin-Binding Protein, Is Involved in Downregulation of β-Catenin , 2002, Molecular and Cellular Biology.

[44]  R. Hay,et al.  SUMO: a history of modification. , 2005, Molecular cell.

[45]  T. Willson,et al.  Parallel SUMOylation-dependent pathways mediate gene- and signal-specific transrepression by LXRs and PPARgamma. , 2007, Molecular cell.

[46]  D. Monté,et al.  SUMO Modification of the Ets-related Transcription Factor ERM Inhibits Its Transcriptional Activity* , 2005, Journal of Biological Chemistry.

[47]  Andrew D Sharrocks,et al.  Dynamic interplay of the SUMO and ERK pathways in regulating Elk-1 transcriptional activity. , 2003, Molecular cell.

[48]  V. G. Panse,et al.  Unconventional tethering of Ulp1 to the transport channel of the nuclear pore complex by karyopherins , 2003, Nature Cell Biology.

[49]  F. Melchior,et al.  A fluorescence resonance energy transfer-based assay to study SUMO modification in solution. , 2005, Methods in enzymology.

[50]  H. Saitoh,et al.  Functional Heterogeneity of Small Ubiquitin-related Protein Modifiers SUMO-1 versus SUMO-2/3* , 2000, The Journal of Biological Chemistry.

[51]  M. Kitagawa,et al.  Characterization of a Novel Mammalian SUMO-1/Smt3-specific Isopeptidase, a Homologue of Rat Axam, Which Is an Axin-binding Protein Promoting β-Catenin Degradation* , 2001, The Journal of Biological Chemistry.

[52]  R. Hay,et al.  SUMO-1 Conjugation in Vivo Requires Both a Consensus Modification Motif and Nuclear Targeting* , 2001, The Journal of Biological Chemistry.

[53]  E. Yeh,et al.  Differential Regulation of Sentrinized Proteins by a Novel Sentrin-specific Protease* , 2000, The Journal of Biological Chemistry.

[54]  L. Zon,et al.  SUMO-1 protease-1 regulates gene transcription through PML. , 2002, Molecular cell.

[55]  M. Matunis,et al.  SUMO Modification of Heterogeneous Nuclear Ribonucleoproteins , 2004, Molecular and Cellular Biology.

[56]  E. Yeh,et al.  Differential Regulation of c-Jun-dependent Transcription by SUMO-specific Proteases* , 2005, Journal of Biological Chemistry.

[57]  M. Tatham,et al.  Polymeric Chains of SUMO-2 and SUMO-3 Are Conjugated to Protein Substrates by SAE1/SAE2 and Ubc9* , 2001, The Journal of Biological Chemistry.

[58]  Zhengxin Wang,et al.  SENP1 Enhances Androgen Receptor-Dependent Transcription through Desumoylation of Histone Deacetylase 1 , 2004, Molecular and Cellular Biology.

[59]  R. Hay,et al.  NEDP1, a Highly Conserved Cysteine Protease That deNEDDylates Cullins* , 2003, Journal of Biological Chemistry.

[60]  Peter O'Hare,et al.  Characterization of the Localization and Proteolytic Activity of the SUMO-specific Protease, SENP1* , 2004, Journal of Biological Chemistry.

[61]  Erica S. Johnson,et al.  The SUMO Isopeptidase Ulp2 Prevents Accumulation of SUMO Chains in Yeast* , 2003, Journal of Biological Chemistry.

[62]  Merlin Crossley,et al.  Modification with SUMO , 2003, EMBO reports.

[63]  S. Au,et al.  Mapping residues of SUMO precursors essential in differential maturation by SUMO-specific protease, SENP1. , 2005, The Biochemical journal.

[64]  R. Hay,et al.  Regulation of Homeodomain-interacting Protein Kinase 2 (HIPK2) Effector Function through Dynamic Small Ubiquitin-related Modifier-1 (SUMO-1) Modification* , 2005, Journal of Biological Chemistry.

[65]  James H Naismith,et al.  SUMO protease SENP1 induces isomerization of the scissile peptide bond , 2006, Nature Structural &Molecular Biology.

[66]  S. Elledge,et al.  The SUMO-1 isopeptidase Smt4 is linked to centromeric cohesion through SUMO-1 modification of DNA topoisomerase II. , 2002, Molecular cell.

[67]  M. Dobson,et al.  The 2μm Plasmid Causes Cell Death in Saccharomyces cerevisiae with a Mutation in Ulp1 Protease , 2005, Molecular and Cellular Biology.

[68]  M. Dasso,et al.  SUMO-2/3 regulates topoisomerase II in mitosis , 2003, The Journal of cell biology.

[69]  M. White,et al.  Broad Spectrum Identification of Cellular Small Ubiquitin-related Modifier (SUMO) Substrate Proteins* , 2004, Journal of Biological Chemistry.