Ratiometric sensing using dual-frequency lifetime discrimination.

1. Byung, S. K., and Colleen, J. (1990) BioTechniques 8, 156–159. 2. Krishnan, B. R., Blakesley, R. W., and Berg, D. E. (1991) Nucleic Acids Res. 19, 1153. 3. Wang, S., Krinks, M., and Moos, M., Jr. (1995) BioTechniques 18, 130–135. 4. Prober, J. M., Trainor, G. L., Dam, R. J., Hobbs, F. W., Robertson, C. W., Zagursky, R. J., Cocuzza, A. J., Jensen, M. A., and Baumeister, K. (1987) Science 238, 336–341. 5. Reeve, M. A., and Fuller, C. W. (1995) Nature 376, 796–797.

[1]  Jan Slavik,et al.  Measurement of individual intracellular pH and membrane potential values in living cells , 1999, Photonics West - Biomedical Optics.

[2]  I Klimant,et al.  Dual lifetime referencing as applied to a chloride optical sensor. , 2001, Analytical chemistry.

[3]  O. Wolfbeis,et al.  Long-lifetime based pH micro-optodes without oxygen interference , 1999 .

[4]  R. Haugland,et al.  Spectral and photophysical studies of benzo[c]xanthene dyes: dual emission pH sensors. , 1991, Analytical biochemistry.

[5]  J. Leroux,et al.  In vitro characterization of a novel polymeric-based pH-sensitive liposome system. , 2000, Biochimica et biophysica acta.

[6]  Peter Lipp,et al.  Ratiometric confocal Ca2+-measurements with visible wavelength indicators in isolated cardiac myocytes , 1993 .

[7]  G. Rao,et al.  Ratiometric oxygen sensing: detection of dual-emission ratio through a single emission filter. , 2000, The Analyst.

[8]  C. Albano,et al.  On-line green fluorescent protein sensor with LED excitation. , 1997, Biotechnology and bioengineering.

[9]  R. Floto,et al.  IgG-induced Ca2+ oscillations in differentiated U937 cells; a study using laser scanning confocal microscopy and co-loaded fluo-3 and fura-red fluorescent probes. , 1995, Cell calcium.

[10]  Yordan Kostov,et al.  Unique Oxygen Analyzer Combining a Dual Emission Probe and a Low-Cost Solid-State Ratiometric Fluorometer , 2000 .

[11]  Joseph R. Lakowicz,et al.  A 10‐GHz frequency‐domain fluorometer , 1990 .

[12]  W. Pulsinelli,et al.  Dicarboxy-dichlorofluorescein: a new fluorescent probe for measuring acidic intracellular pH. , 1990, Analytical biochemistry.

[13]  W. Stemmer,et al.  Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling , 1996, Nature Biotechnology.

[14]  A. Verkman,et al.  Synthesis and characterization of dual-wavelength Cl--sensitive fluorescent indicators for ratio imaging. , 1999, American journal of physiology. Cell physiology.

[15]  G. Rao,et al.  Low-cost microbioreactor for high-throughput bioprocessing. , 2001, Biotechnology and bioengineering.

[16]  A S Verkman,et al.  Green fluorescent protein as a noninvasive intracellular pH indicator. , 1998, Biophysical journal.

[17]  J. Lakowicz.  Principles of fluorescence spectroscopy , 1983 .

[18]  P. Rabinovitch,et al.  Chapter 10 Intracellular Ionized Calcium , 1994 .

[19]  J. Lakowicz,et al.  Fluorescence properties of rhodamine 800 in whole blood and plasma. , 2000, Analytical biochemistry.