Optical Image Improvement in Biomedical Electron Microscopy and Ultrasonics

The imaging improvement method recently proposed by the authors for transmission electron microscopy in the general case has now been successfully demonstrated with electron micrographs of a bacteriophage virus and of carbon-foil test specimens originally photographed with heretofore unsurmountable blurring. The original electron micrographs were recorded on two commercial high resolution electron microscopes. The theoretical limit of diffraction attainable with the microscopes used has been obtained by removal of artifacts, of contrast inversions, and of amplitude attenuations in the transfer function resulting from the unavoidable aberrations and the use of the needed defocusing phase contrast.