Influence of Copper Oxide Nanoparticles on Gene Expression of Birch Clones In Vitro under Stress Caused by Phytopathogens

Recently, metal oxide nanoparticles (NPs) have attracted attention as promising components for the protection and stimulation of plant microclones in tissue culture in vitro. However, the effect of NPs on the genetic mechanisms underlying plant adaptive responses remains poorly understood. We studied the effect of column-shaped CuO NPs 50 nm in diameter and 70–100 nm in length at a concentration of 0.1–10 mg/L on the development of phytopathogenic fungi Alternaria alternata, Fusarium oxysporum, and Fusarium avenaceum in culture, as well as on the infection of downy birch micro-clones with phytopathogens and the level of genes expression associated with the formation of plant responses to stress induced by microorganisms. CuO NPs effectively suppressed the development of colonies of phytopathogenic fungi A. alternata and F. avenaceum (up to 68.42% inhibition at 10 mg/L CuO NPs) but not the development of a colony of F. oxysporum. Exposure to the NPs caused multidirectional responses at the level of plant genes transcription: 5 mg/L CuO NPs significantly increased the expression level of the LEA8 and MYB46 genes and decreased the expression of DREB2 and PAL. Infection with A. alternata significantly increased the level of MYB46, LEA8, PAL, PR-1, and PR-10 transcripts in birch micro-clones; however, upon exposure to a medium with NPs and simultaneous exposure to a phytopathogen, the expression of the MYB46, PR-1, and PR-10 genes decreased by 5.4 times, which is associated with a decrease in the pathogenic load caused by the effect of NPs and the simultaneous stimulation of clones in vitro. The results obtained can be used in the development of preparations based on copper oxide NPs for disinfection and stimulation of plant phytoimmunity during clonal micropropagation of tree crops.

[1]  Saqib Farooq,et al.  Interplay of ZnONPs and/or SeNPs induces arsenic tolerance in soybean by regulation of antioxidants pool, WRKY genes, and expression of arsenic transporters , 2022, Environmental and Experimental Botany.

[2]  P. Evlakov,et al.  Optimized method for RNA extraction from leaves of forest tree species , 2021, IOP Conference Series: Earth and Environmental Science.

[3]  C. Giannini,et al.  Nano-Enable Materials Promoting Sustainability and Resilience in Modern Agriculture , 2021, Nanomaterials.

[4]  Izabela Jośko,et al.  Combined effect of nano-CuO and nano-ZnO in plant-related system: From bioavailability in soil to transcriptional regulation of metal homeostasis in barley. , 2021, Journal of hazardous materials.

[5]  A. Tsouknidas,et al.  Synthesis and Characterization of Novel Copper Nanoparticles for the Control of Leaf Spot and Anthracnose Diseases of Olive , 2021, Nanomaterials.

[6]  M. Kornaros,et al.  Nanobiotechnological advancements in agriculture and food industry: Applications, nanotoxicity, and future perspectives. , 2021, The Science of the total environment.

[7]  A. Gusev,et al.  Titanium Trisulfide Nanoribbons Affect the Downy Birch and Poplar × Aspen Hybrid in Plant Tissue Culture via the Emission of Hydrogen Sulfide , 2021 .

[8]  S. Garcia-Sanchez,et al.  Nanoimpact in Plants: Lessons from the Transcriptome , 2021, Plants.

[9]  V. Voliani,et al.  Antimicrobial Nano-Agents: The Copper Age , 2021, ACS nano.

[10]  O. Fedorova,et al.  Effect of Copper Oxide and Silver Nanoparticles on the Development of Tolerance to Alternaria alternata in Poplar in Vitro Clones , 2021, Nanobiotechnology Reports.

[11]  I. Torres-Pacheco,et al.  Nanoparticles as Novel Elicitors to Improve Bioactive Compounds in Plants , 2021, Agriculture.

[12]  Mengmeng Rui,et al.  OsFTIP7 determines metallic oxide nanoparticles response and tolerance by regulating auxin biosynthesis in rice. , 2021, Journal of hazardous materials.

[13]  I. Kokina,et al.  Effect of Fe3O4 and CuO Nanoparticles on Morphology, Genotoxicity, and miRNA Expression on Different Barley (Hordeum vulgare L.) Genotypes , 2021, TheScientificWorldJournal.

[14]  Harshal P. Mahajan,et al.  Green Synthesis of Chitosan Capped-Copper Nano Biocomposites: Synthesis, Characterization, and Biological Activity against Plant Pathogens , 2021, BioNanoScience.

[15]  S. Iqbal,et al.  Alleviation Mechanism of Drought Stress in Plants Using Metal Nanoparticles – A Perspective Analysis , 2021, Sustainable Agriculture Reviews 53.

[16]  U. Farooq,et al.  Abiotic stresses induced physiological, biochemical, and molecular changes in Betula platyphylla: a review , 2021 .

[17]  G. Manchanda,et al.  Nanoparticles in sustainable agriculture: An emerging opportunity. , 2020, Journal of controlled release : official journal of the Controlled Release Society.

[18]  O. Morsy,et al.  Transcriptomic changes in green bean pods against grey mould and white rot diseases via field application of chemical elicitor nanoparticles. , 2020, IET nanobiotechnology.

[19]  O. Fedorova,et al.  Influence of Copper Oxide and Silver Nanoparticles on Microclonal Sprouts of Downy Birch (Betula pubescens Ehrh.) , 2020, Nanotechnologies in Russia.

[20]  T. M. Linh,et al.  Metal-Based Nanoparticles Enhance Drought Tolerance in Soybean , 2020 .

[21]  K. S. Siddiqi,et al.  Current status of plant metabolite-based fabrication of copper/copper oxide nanoparticles and their applications: a review , 2020, Biomaterials Research.

[22]  J. Daròs,et al.  Reduced Graphene Oxide Nanosheet-Decorated Copper Oxide Nanoparticles: A Potent Antifungal Nanocomposite against Fusarium Root Rot and Wilt Diseases of Tomato and Pepper Plants , 2020, Nanomaterials.

[23]  Yan-fang Ren,et al.  Phytotoxicity Assessment of Copper Oxide Nanoparticles on the Germination, Early Seedling Growth, and Physiological Responses in Oryza sativa L. , 2020, Bulletin of Environmental Contamination and Toxicology.

[24]  V. Yadav,et al.  Phenylpropanoid Pathway Engineering: An Emerging Approach towards Plant Defense , 2020, Pathogens.

[25]  R. Darvishzadeh,et al.  Fe2 O3 NPs induced biochemical responses and expression of genes involved in rosmarinic acid biosynthesis pathway in Moldavian balm under salinity stress. , 2020, Physiologia plantarum.

[26]  K. Acharya,et al.  Green Synthesized Copper Oxide Nanoparticles Ameliorate Defence and Antioxidant Enzymes in Lens culinaris , 2020, Nanomaterials.

[27]  Liqun Li,et al.  Genome-wide identification and characterization of late embryogenesis abundant protein-encoding gene family in wheat: evolution and expression profiles during development and stress. , 2020, Gene.

[28]  A. Gusev,et al.  Photocatalytically Active Zinc Oxide and Titanium Dioxide Nanoparticles in Clonal Micropropagation of Plants: Prospects , 2019, Nanotechnologies in Russia.

[29]  N. Kavroulakis,et al.  Use of copper, silver and zinc nanoparticles against foliar and soil-borne plant pathogens. , 2019, The Science of the total environment.

[30]  Daoyuan Zhang,et al.  A Gene Regulatory Network Controlled by BpERF2 and BpMYB102 in Birch under Drought Conditions , 2019, International journal of molecular sciences.

[31]  H. AbdElgawad,et al.  NiO-nanoparticles induce reduced phytotoxic hazards in wheat (Triticum aestivum L.) grown under future climate CO2. , 2019, Chemosphere.

[32]  M. Chandrasekaran,et al.  Chitosan and chitosan nanoparticles induced expression of pathogenesis-related proteins genes enhances biotic stress tolerance in tomato. , 2019, International journal of biological macromolecules.

[33]  I. Chung,et al.  Effect of Copper Oxide Nanoparticles on the Physiology, Bioactive Molecules, and Transcriptional Changes in Brassica rapa ssp. rapa Seedlings , 2019, Water, Air, & Soil Pollution.

[34]  V. K. Mishra,et al.  Plant-Mediated Synthesis of Copper Oxide Nanoparticles and Their Biological Applications , 2019, Nanomaterials and Plant Potential.

[35]  J. Peralta-Videa,et al.  Toxicity of copper hydroxide nanoparticles, bulk copper hydroxide, and ionic copper to alfalfa plants: A spectroscopic and gene expression study. , 2018, Environmental pollution.

[36]  Kareem A. Mosa,et al.  Copper Nanoparticles Induced Genotoxicty, Oxidative Stress, and Changes in Superoxide Dismutase (SOD) Gene Expression in Cucumber (Cucumis sativus) Plants , 2018, Front. Plant Sci..

[37]  K. Shameli,et al.  Bactericidal Properties of Plants-Derived Metal and Metal Oxide Nanoparticles (NPs) , 2018, Molecules.

[38]  D. Cowan,et al.  LEA Proteins and the Evolution of the WHy Domain , 2018, Applied and Environmental Microbiology.

[39]  M. Takita,et al.  Persistence in Phytopathogenic Bacteria: Do We Know Enough? , 2018, Front. Microbiol..

[40]  A. Anderson,et al.  Remodeling of root morphology by CuO and ZnO nanoparticles: effects on drought tolerance for plants colonized by a beneficial pseudomonad. , 2018 .

[41]  L. Pagano,et al.  Effect of Metalloid and Metal Oxide Nanoparticles on Fusarium Wilt of Watermelon. , 2018, Plant disease.

[42]  Rijuta Ganesh Saratale,et al.  Recent developments in nanotechnology transforming the agricultural sector: a transition replete with opportunities. , 2018, Journal of the science of food and agriculture.

[43]  A. Anderson,et al.  CuO and ZnO Nanoparticles Modify Interkingdom Cell Signaling Processes Relevant to Crop Production. , 2017, Journal of agricultural and food chemistry.

[44]  D. Jain,et al.  Role of Pathogenesis-Related (PR) Proteins in Plant Defense Mechanism , 2018 .

[45]  H. Qian,et al.  Physiological and Molecular Response of Arabidopsis thaliana to CuO Nanoparticle (nCuO) Exposure , 2017, Bulletin of Environmental Contamination and Toxicology.

[46]  T. Vanek,et al.  Transcriptomic Response of Arabidopsis thaliana Exposed to CuO Nanoparticles, Bulk Material, and Ionic Copper. , 2017, Environmental science & technology.

[47]  Zetian Zhang,et al.  Potential Applications and Antifungal Activities of Engineered Nanomaterials against Gray Mold Disease Agent Botrytis cinerea on Rose Petals , 2017, Front. Plant Sci..

[48]  C. Sheeba,et al.  Nanoparticles Alter Secondary Metabolism in Plants via ROS Burst , 2017, Front. Plant Sci..

[49]  L. Pagano,et al.  Weathering in soil increases nanoparticle CuO bioaccumulation within a terrestrial food chain , 2017, Nanotoxicology.

[50]  J. Grosser,et al.  Potential use of the DREB/ERF, MYB, NAC and WRKY transcription factors to improve abiotic and biotic stress in transgenic plants , 2017, Plant Cell, Tissue and Organ Culture (PCTOC).

[51]  A. Fahmy,et al.  Copper Sulfate Nanoparticales In vitro Applications on Verbena bipinnatifida Nutt . Stimulating Growth and Total Phenolic Content Increasments , 2017 .

[52]  J. White,et al.  CuO Nanoparticle Interaction with Arabidopsis thaliana: Toxicity, Parent-Progeny Transfer, and Gene Expression. , 2016, Environmental science & technology.

[53]  G. Cadenas-Pliego,et al.  Cu Nanoparticles absorbed on chitosan hydrogels positively alter morphological, production, and quality characteristics of tomato , 2016 .

[54]  A. I. Ustinov,et al.  The Influence of Cu и Co Nanoparticles on Growth Characteristics and Biochemical Structure of Mentha Longifolia In Vitro , 2016 .

[55]  I. Chung,et al.  Biochemical, anatomical and molecular level changes in cucumber (Cucumis sativus) seedlings exposed to copper oxide nanoparticles , 2015, Biologia.

[56]  Sebastian Schornack,et al.  Oomycete Interactions with Plants: Infection Strategies and Resistance Principles , 2015, Microbiology and Molecular Reviews.

[57]  I. Chung,et al.  Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.). , 2015, Ecotoxicology and environmental safety.

[58]  H. Xin,et al.  The effect of CuO NPs on reactive oxygen species and cell cycle gene expression in roots of rice , 2015, Environmental toxicology and chemistry.

[59]  N. Padmavathy,et al.  Understanding the pathway of antibacterial activity of copper oxide nanoparticles , 2015 .

[60]  I. Chung,et al.  A Mechanistic Study on the Toxic Effect of Copper Oxide Nanoparticles in Soybean (Glycine max L.) Root Development and Lignification of Root Cells , 2014, Biological Trace Element Research.

[61]  M. Hahn.  The rising threat of fungicide resistance in plant pathogenic fungi: Botrytis as a case study , 2014, Journal of chemical biology.

[62]  I. O. Wallinder,et al.  Cell membrane damage and protein interaction induced by copper containing nanoparticles--importance of the metal release process. , 2013, Toxicology.

[63]  P. Vera,et al.  MYB46 Modulates Disease Susceptibility to Botrytis cinerea in Arabidopsis12[W] , 2011, Plant Physiology.

[64]  Holger Moch,et al.  Nanoparticle cytotoxicity depends on intracellular solubility: comparison of stabilized copper metal and degradable copper oxide nanoparticles. , 2010, Toxicology letters.

[65]  Feng Chen,et al.  Statistical analysis of real-time PCR data , 2006, BMC Bioinformatics.

[66]  R. Shields,et al.  The toxicity of antibiotics to plant cell cultures , 1983, Plant Cell Reports.

[67]  Xiaoling Yu,et al.  Internal bacterial contamination of micropropogated hazelnut: identification and antibiotic treatment , 2004, Plant Cell, Tissue and Organ Culture.