Applications of Plasmid Electrotransfer

The use of electric pulses to transfect cells has recently been extended to show the utility of this procedure in vivo. Electrotransfer has been performed in vivo on several tissue types including skin, blood vessels, liver, tumor, muscle, cornea, brain and spleen. The most widely targeted tissue has been skeletal muscle. In addition to its potential use in gene therapy, in vivo DNA electrotransfer is also, because of its simplicity, a powerful laboratory tool to study in vivo gene expression and function in a given tissue. Many published studies have now shown that plasmid electrotransfer can lead to a long-lasting therapeutic effect in various pathologies, such as cancer, blood disease, or muscle ischemia. The future potential for this gene therapy approach will include delivery for both local action or distal effect by secretion of the transgenic proteins in the circulation.

[1]  X. Zhang,et al.  Regression of tumors by IFN-α electroporation gene therapy and analysis of the responsible genes by cDNA array , 2002, Gene Therapy.

[2]  T. Nishi,et al.  Targeted gene transfer to corneal endothelium in vivo by electric pulse , 1998, Gene Therapy.

[3]  J. Miyazaki,et al.  Gene transfer of Fc-fusion cytokine by in vivo electroporation: application to gene therapy for viral myocarditis , 2002, Gene Therapy.

[4]  Darius Moradpour,et al.  In vivo gene electroinjection and expression in rat liver , 1996, FEBS letters.

[5]  M. Bureau,et al.  Electric pulses increase the immunogenicity of an influenza DNA vaccine injected intramuscularly in the mouse. , 2001, Vaccine.

[6]  Toshiyuki Matsuzaki,et al.  Direct gene transfer into rat liver cells by in vivo electroporation , 1998, FEBS letters.

[7]  M. Bureau,et al.  Non-viral gene transfer of murine spleen cells achieved by in vivo electroporation , 2003, Gene Therapy.

[8]  M. Bureau,et al.  Protective role of interleukin-10 in atherosclerosis. , 1999, Circulation research.

[9]  D. Scherman,et al.  Antisense MBD2 gene therapy inhibits tumorigenesis , 2002, The journal of gene medicine.

[10]  M. Jaroszeski,et al.  In vivo electroporation of plasmids encoding GM-CSF or interleukin-2 into existing B16 melanomas combined with electrochemotherapy induces long-term antitumour immunity , 2000, Melanoma research.

[11]  H. Hirai,et al.  In vivo electroporation-mediated transfer of interleukin-12 and interleukin-18 genes induces significant antitumor effects against melanoma in mice , 2001, Gene Therapy.

[12]  D. Coppola,et al.  IL-12 plasmid delivery by in vivo electroporation for the successful treatment of established subcutaneous B16.F10 melanoma. , 2002, Molecular therapy : the journal of the American Society of Gene Therapy.

[13]  G. Prud’homme,et al.  Immunoinhibitory DNA vaccine protects against autoimmune diabetes through cDNA encoding a selective CTLA-4 (CD152) ligand. , 2002, Human gene therapy.

[14]  I. Goldberg,et al.  Inhibition of Wilms' tumor growth by intramuscular administration of tissue inhibitor of metalloproteinases-4 plasmid DNA , 2001, Oncogene.

[15]  H. Stedman,et al.  Modular Organization of Phylogenetically Conserved Domains Controlling Developmental Regulation of the Human Skeletal Myosin Heavy Chain Gene Family* , 2002, The Journal of Biological Chemistry.

[16]  M. Rols,et al.  In vivo electrically mediated protein and gene transfer in murine melanoma , 1998, Nature Biotechnology.

[17]  S. Sukharev,et al.  In vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA. , 1991, Biochimica et biophysica acta.

[18]  Xinjian Zhang,et al.  Regression of tumor growth and induction of long-term antitumor memory by interleukin 12 electro-gene therapy. , 2002, Journal of the National Cancer Institute.

[19]  J. Miyazaki,et al.  Long-term production of erythropoietin after electroporation-mediated transfer of plasmid DNA into the muscles of normal and uremic rats , 2001, Gene Therapy.

[20]  D. Scherman,et al.  Erythropoietin secretion and physiological effect in mouse after intramuscular plasmid DNA electrotransfer , 1999, Journal of Gene Medicine.

[21]  J. Miyazaki,et al.  Electroporation-mediated interleukin-12 gene therapy for hepatocellular carcinoma in the mice model. , 2001, Cancer research.

[22]  M. Bureau,et al.  High-efficiency gene transfer into skeletal muscle mediated by electric pulses. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[23]  V. Deleuze,et al.  Efficacy of interleukin‐10 gene electrotransfer into skeletal muscle in mice with collagen‐induced arthritis , 2003, The journal of gene medicine.

[24]  R. Cortese,et al.  Efficient and regulated erythropoietin production by naked DNA injection and muscle electroporation. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[25]  L. Mir,et al.  Efficient DNA electrotransfer into tumors. , 2000, Bioelectrochemistry.

[26]  S. Gerson,et al.  Cancer gene therapy: scientific basis. , 2002, Annual review of medicine.

[27]  N. Nakatsuji,et al.  Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. , 2001, Developmental biology.

[28]  J. Suen,et al.  Intramuscular electroporation delivery of IFN-α gene therapy for inhibition of tumor growth located at a distant site , 2001, Gene Therapy.

[29]  M. Shibata,et al.  Suppression of murine mammary carcinoma growth and metastasis by HSVtk/GCV gene therapy using in vivo electroporation , 2002, Cancer Gene Therapy.

[30]  D. Scherman,et al.  Improvement of mouse β-thalassemia by electrotransfer of erythropoietin cDNA , 2001 .

[31]  J. Miyazaki,et al.  Electroporation-mediated tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)/Apo2L gene therapy for hepatocellular carcinoma. , 2002, Human gene therapy.

[32]  D. Scherman,et al.  Electrotransfer of naked DNA in the skeletal muscles of animal models of muscular dystrophies , 2001, Gene Therapy.

[33]  F. McCormick,et al.  Cancer gene therapy: fringe or cutting edge? , 2001, Nature Reviews Cancer.

[34]  H. Aihara,et al.  Gene transfer into muscle by electroporation in vivo. , 1998, Nature biotechnology.

[35]  D. Scherman,et al.  Oxygen tension and a pharmacological switch in the regulation of transgene expression for gene therapy , 2001, The journal of gene medicine.

[36]  I. Mathiesen.  Electropermeabilization of skeletal muscle enhances gene transfer in vivo , 1999, Gene Therapy.

[37]  J. Ulmer,et al.  Increased DNA Vaccine Delivery and Immunogenicity by Electroporation In Vivo , 2000, The Journal of Immunology.

[38]  E. Neumann,et al.  Gene transfer into mouse lyoma cells by electroporation in high electric fields. , 1982, The EMBO journal.

[39]  David A. Dean,et al.  Gene Transfer to Intact Mesenteric Arteries by Electroporation , 2000, Journal of Vascular Research.

[40]  D. Scherman,et al.  High-level protein secretion into blood circulation after electric pulse-mediated gene transfer into skeletal muscle. , 2000, Molecular therapy : the journal of the American Society of Gene Therapy.

[41]  T. Nishi,et al.  Intratumoral delivery of interleukin 12 expression plasmids with in vivo electroporation is effective for colon and renal cancer. , 2001, Human gene therapy.

[42]  R. Cortese,et al.  Stringent control of gene expression in vivo by using novel doxycycline-dependent trans-activators. , 2002, Human gene therapy.

[43]  D. Freyssenet,et al.  High-efficiency gene electrotransfer into skeletal muscle: description and physiological applicability of a new pulse generator. , 2002, Biochemical and biophysical research communications.

[44]  J. Miyazaki,et al.  Protection Against Autoimmune Myocarditis by Gene Transfer of Interleukin-10 by Electroporation , 2001, Circulation.

[45]  H. Potter,et al.  Electroporation in biology: methods, applications, and instrumentation. , 1988, Analytical biochemistry.

[46]  L. Heller,et al.  Effect of Electrically Mediated Intratumor and Intramuscular Delivery of a Plasmid Encoding IFN α on Visible B16 Mouse Melanomas , 2002, Technology in cancer research & treatment.

[47]  M. Bureau,et al.  Long-term, high level in vivo gene expression after electric pulse-mediated gene transfer into skeletal muscle. , 1998, Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie.

[48]  K. Dono,et al.  Attenuated acute liver injury in mice by naked hepatocyte growth factor gene transfer into skeletal muscle with electroporation , 2002, Gut.

[49]  R. Schwartz,et al.  Myogenic expression of an injectable protease-resistant growth hormone–releasing hormone augments long-term growth in pigs , 1999, Nature Biotechnology.

[50]  D. Bohl,et al.  betaMinor-globin messenger RNA accumulation in reticulocytes governs improved erythropoiesis in beta thalassemic mice after erythropoietin complementary DNA electrotransfer in muscles. , 2001, Blood.

[51]  A. Benabid,et al.  Muscle transfection by electroporation with high-voltage and short-pulse currents provides high-level and long-lasting gene expression. , 2000, Human gene therapy.