Multifocal Multi-Photon Microscopy

Multi-photon processes relying on the cooperative action of two or more photons can broadly be divided into two families that are distinguished by the fact that the photons are either absorbed or scattered (Shen, 1984). Whereas the scattering events relevant to microscopy are second and third harmonic generation (SHG, THG), as well as coherent anti-Stokes Raman scattering (CARS), the useful multi-photon absorption events are two- and threephoton excitation (2PE, 3PE).

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