KINETICS AND PATHWAYS OF OXIDATION OF O-PHENYLENEDIAMINE, 3,3'-DIMETHOXYBENZIDINE, AND 3,3', 5,5'-TETRAMETHYLBENZIDINE WITH HYDROGEN PEROXIDE, CATALYZ ED WITH HORSE RADISH PEROXIDASE IMMOBILIZED ON VARIOUS SUPPORTS

Products of oxidation of o-phenylenediamine, 3,3`-dimethoxybenzidine, and 3,3 ,5,5`-tetramethylbenzidine with hydrogen peroxide, catalyzed with peroxidase from horse radish roots (native and immobilized on chromatographic paper and polyurethane foam) were studied in comparison by absorption and diffuse reflection spectroscopy. The kinetics and pathways of the enzyme-catalyzed reactions differ depending on whether the reaction was performed in solution with native peroxidase or on the above-mentioned supports with the immobilized enzyme.