Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain.

Axin is a negative regulator of the canonical Wnt signalling pathway that mediates the phosphorylation of beta-catenin by glycogen synthase kinase 3beta. The DIX domain of rat axin, which is important for its homooligomerization and interactions with other regulators in the Wnt pathway, was purified and crystallized by the sitting-drop vapour-diffusion technique using polyethylene glycol 6000 and lithium sulfate as crystallization agents. Crystals belong to space group P6(1) or P6(5), with unit-cell parameters a = b = 91.49, c = 84.92 A. An X-ray diffraction data set has been collected to a nominal resolution of 2.9 A.

[1]  D. Capelluto,et al.  Secondary structure, 1H, 13C and 15N resonance assignments and molecular interactions of the dishevelled DIX domain. , 2005, Journal of biochemistry and molecular biology.

[2]  Sheng-Cai Lin,et al.  Axin: A Master Scaffold for Multiple Signaling Pathways , 2004, Neurosignals.

[3]  M. Masu,et al.  Ccd1, a Novel Protein with a DIX Domain, Is a Positive Regulator in the Wnt Signaling during Zebrafish Neural Patterning , 2003, Current Biology.

[4]  M. Overduin,et al.  The DIX domain targets dishevelled to actin stress fibres and vesicular membranes , 2002, Nature.

[5]  F. Costantini,et al.  Domains of axin and disheveled required for interaction and function in wnt signaling. , 2000, Biochemical and biophysical research communications.

[6]  Jiahuai Han,et al.  Dimerization Choices Control the Ability of Axin and Dishevelled to Activate c-Jun N-terminal Kinase/Stress-activated Protein Kinase* , 2000, The Journal of Biological Chemistry.

[7]  A. Kikuchi,et al.  Roles of Axin in the Wnt signalling pathway. , 1999, Cellular signalling.

[8]  A. Kikuchi,et al.  Modulation of Wnt signaling by Axin and Axil. , 1999, Cytokine & growth factor reviews.

[9]  J Mao,et al.  Axin and Frat1 interact with Dvl and GSK, bridging Dvl to GSK in Wnt‐mediated regulation of LEF‐1 , 1999, The EMBO journal.

[10]  Akira Kikuchi,et al.  DIX Domains of Dvl and Axin Are Necessary for Protein Interactions and Their Ability To Regulate β-Catenin Stability , 1999, Molecular and Cellular Biology.

[11]  L. Williams,et al.  Functional Domains of Axin , 1999, The Journal of Biological Chemistry.

[12]  F. Costantini,et al.  Identification of a Domain of Axin That Binds to the Serine/Threonine Protein Phosphatase 2A and a Self-binding Domain* , 1999, The Journal of Biological Chemistry.

[13]  J Mao,et al.  Dishevelled Proteins Lead to Two Signaling Pathways , 1999, The Journal of Biological Chemistry.

[14]  Stephen W. Byers,et al.  Serine Phosphorylation-regulated Ubiquitination and Degradation of β-Catenin* , 1997, The Journal of Biological Chemistry.

[15]  Jörg Stappert,et al.  β‐catenin is a target for the ubiquitin–proteasome pathway , 1997 .

[16]  Z. Otwinowski,et al.  [20] Processing of X-ray diffraction data collected in oscillation mode. , 1997, Methods in enzymology.