PHYTOHORMONE INDUCED CHANGES ON BONE HISTOLOGY IN NORMAL AND DIABETIC MALE RAT

Objective: To study the effect of brasslinolide keto isoform 28-homocastasterone in diabetic male rat bone marrow cells, bone histological changes, electrolytes and alkaline phosphatase activity in rat blood. Methods: Diabetes was induced in the group (n=6) of rats with a single peritoneal injection of streptozotocin (60 mg/kg bwt). With a treatment schedule of 15 consecutive days, control (n=6) and diabetic rats received 666µg/kg bwt of 28-homocastasterone. Circulating blood cell count, cell indices, bone marrow cells, bone histology, electrolytes Na + , K + , Cl - , P, Ca 2+ and alkaline phosphatase activity was assessed. Results: Cytological examination showed an increased erythrocyte progenitor and megakaryocyte cell lineage along with improved osteoblastic and bone histomorphology in the treated group was noted. A significant reduction in electrolytes level (p<0.05) and increased in alkaline phosphatase activity (p<0.05) was noted in treated groups. Conclusion: It is suggested that brassinosteroid keto isoform 28-homocastasterone exhibits a hematopoietic effect in diabetic rat and improves bone histology while reducing hyperglycemic damage in bone.