Further studies on the control of ACh sensitivity by muscle activity in the rat.

1. Denervated rat soleus muscles were stimulated directly through chronically implanted electrodes and the influence of different amounts and patterns of stimuli on the acetylcholine (ACh) sensitivity of the muscle was studied. The number of stimuli was varied by giving similar trains of stimuli (10 Hz for 10 sec) at different intervals (0 to 12 hr). The pattern of stimulation was varied by giving different trains of stimuli (100 Hz for 1 sec, 10 Hz for 10 sec and 1 Hz continuously) as the same average frequency of stimulation (1 Hz). 2. Stimulation usually started 5 days after the denervation when ACh hypersensitivity was fully developed. Most stimulation procedures reduced extrajunctional ACh sensitivity to normal or below normal values within 5‐21 days, and these levels were maintained on prolonged stimulation. 3. The rate at which ACh hypersensitivity disappeared increased with increasing amount and frequency of stimulation. However, as few as 100 stimuli given every 5‐5 hr for 3 weeks caused a tenfold reduction of sensitivity. 4. The stimulation had little or no effect on the ACh sensitivity at the end plate. Along the rest of the fibre the sensitivity was reduced at approximately the same rate except near the tendons where it appeared to fall more slowly in some fibres. 5. The stimulation restored the resting membrane potential of the denervated fibres to normal.

[1]  E. Frank,et al.  The interaction between foreign and original motor nerves innervating the soleus muscle of rats. , 1975, The Journal of physiology.

[2]  Norway,et al.  Contractile properties of muscle: control by pattern of muscle activity in the rat , 1974, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[3]  J. Steinbach,et al.  Role of Muscle Activity in Nerve-Muscle Interaction in vitro , 1974, Nature.

[4]  S. Thesleff.  PHYSIOLOGICAL EFFECTS OF DENERVATION OF MUSCLE * , 1974 .

[5]  D. Drachman.  THE ROLE OF ACETYLCHOLINE AS A NEUROTROPHIC TRANSMITTER * , 1974 .

[6]  G. Vrbóva,et al.  Two factors responsible for the development of denervation hypersensitivity , 1974, The Journal of physiology.

[7]  B. Sakmann,et al.  The effect of contractile activity on fibrillation and extrajunctional acetylcholine‐sensitivity in rat muscle maintained in organ culture , 1974, The Journal of physiology.

[8]  P. Waser,et al.  Electron microscopic studies of degeneration and regeneration of rat neuromuscular junctions. , 1973, Brain research.

[9]  J. Jansen,et al.  Hyperinnervation of Skeletal Muscle Fibers: Dependence on Muscle Activity , 1973, Science.

[10]  G. Fischbach,et al.  Regulation of Muscle Acetylcholine Sensitivity by Muscle Activity in Cell Culture , 1973, Science.

[11]  D. Drachman,et al.  Trophic Regulation of Acetylcholine Sensitivity of Muscle: Effect of Electrical Stimulation , 1972, Science.

[12]  T. Lømo,et al.  Control of ACh sensitivity by muscle activity in the rat , 1972, The Journal of physiology.

[13]  G. Vrbóva,et al.  Effect of muscle activity on denervation hypersensitivity. , 1970, The Journal of physiology.

[14]  D. Fambrough.  Acetylcholine Sensitivity of Muscle Fiber Membranes: Mechanism of Regulation by Motoneurons , 1970, Science.

[15]  T K Chowdhury,et al.  Fabrication of extremely fine glass micropipette electrodes. , 1969, Journal of scientific instruments.

[16]  G. Fischbach,et al.  Changes in contractile properties of disused soleus muscles , 1969, The Journal of physiology.

[17]  J. Heaton,et al.  A quantitative study of cholinesterase in myoneural junctions from rat and guinea‐pig extraocular muscles , 1968, The Journal of physiology.

[18]  E. Albuquerque,et al.  A comparative study of membrane properties of innervated and chronically denervated fast and slow skeletal muscles of the rat. , 1968, Acta physiologica Scandinavica.

[19]  A. Bahler,et al.  The Dynamic Properties of Mammalian Skeletal Muscle , 1968, The Journal of general physiology.

[20]  R. Miledi,et al.  Electrophysiology and electron-microscopy of rat neuromuscular junctions after nerve degeneration , 1968, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[21]  R. Miledi,et al.  Sensitivity to Acetylcholine in Rat Slow Muscle , 1966, Nature.

[22]  C. Eyzaguirre,et al.  Pacemaker site of fibrillation potentials in denervated mammmalian muscle. , 1966, Journal of neurophysiology.

[23]  N. Emmelin,et al.  DEVELOPMENT OF SUPERSENSITIVITY AS DEPENDENT ON THE LENGTH OF DEGENERATING NERVE FIBRES. , 1965, Quarterly journal of experimental physiology and cognate medical sciences.

[24]  R. Close.  Dynamic properties of fast and slow skeletal muscles of the rat during development , 1964, The Journal of physiology.

[25]  B. Katz,et al.  Further observations on the distribution of acetylcholine‐reactive sites in skeletal muscle , 1964, The Journal of physiology.

[26]  S. Thesleff,et al.  Effects of motor inactivation on the chemical sensitivity of skeletal muscle. , 1961, Acta physiologica Scandinavica.

[27]  R. Miledi.  The acetylcholine sensitivity of frog muscle fibres after complete or partial denervation , 1960, The Journal of Physiology.

[28]  S. Thesleff,et al.  A study of supersensitivity in denervated mammalian skeletal muscle , 1959, The Journal of physiology.

[29]  R. H. Adrian,et al.  The effect of internal and external potassium concentration on the membrane potential of frog muscle , 1956, The Journal of physiology.

[30]  D. Y. Solandt,et al.  A COMPARISON OF EFFECTS OF UPPER AND LOWER MOTOR NEURONE LESIONS ON SKELETAL MUSCLE , 1942 .

[31]  T. Lømo,et al.  Control of ACh sensitivity in rat muscle fibers. , 1976, Cold Spring Harbor symposia on quantitative biology.

[32]  T. LØmo.  Neurotrophic control of colchicine effects on muscle? , 1974, Nature.

[33]  A. Harris,et al.  Inductive functions of the nervous system. , 1974, Annual review of physiology.

[34]  L. Landmesser.  Pharmacological properties, cholinesterase activity and anatomy of nerve—muscle junctions in vagus‐innervated frog sartorius , 1972, The Journal of physiology.

[35]  E. Albuquerque,et al.  Fast and slow mammalian muscles after denervation. , 1970, Experimental neurology.

[36]  C. Eyzaguirre,et al.  Fibrillation and hypersensitivity to ACh in denervated muscle: effect of length of degenerating nerve fibers. , 1955, Journal of neurophysiology.

[37]  D. Y. Solandt,et al.  THE EFFECT OF SKELETAL FIXATION ON SKELETAL MUSCLE , 1943 .

[38]  S. S. Tower.  THE REACTION OF MUSCLE TO DENERVATION , 1939 .

[39]  M. Walski,et al.  Electron-microscopic Studies , 2022 .