Isolation and Identification of Aspergillus fumigatus from Brooder Pneumonia Affected Broiler Chicken

Brooder pneumonia is a common respiratory problem during brooding period of broiler chicken in high humid region. It is caused by fungal genus Aspergillus, specially Aspergillus fumigatus. The present study was carried out the isolation and identification of Aspergillus fumigatus from brooder pneumonia affected broiler chicken at 10 days old. The characteristic grayish colors cottony colony of A. fumigatus was isolated by culture the yellowish spherical caseous granulomatous lungs nodules in potato dextrose agar media. In histopathological examination the characteristic eosinophilic hyphae of A. fumigatus was demonstrated in the coagulative necrotic center of granulomatous nodules of lungs by routine Hematoxylin and Eosin staining procedure. Finally, the 26S gene of A. fumigatus was identified by amplification of 401-bp fragment by PCR test.