Effect of Ultra-high-pressure Homogenization on Structure and on Rheological Properties of Soy Protein-stabilized Emulsions

An ultra high-pressure homogenizer (20 to 350 MPa) was used to realize fine food emulsions stabilized by soy proteins. The first aim of the work was to understand how dynamic high-pressure processing affects soybean globulin conformation. Then, the effect of homogenizing pressure on the emulsions structure and rheology was investigated. High-pressure homogenization caused denaturation of proteins due to strong mechanical forces and high temperatures encountered in the valve. Droplet sizes of emulsions were greatly reduced with high-pressure homogenization and Newtonian liquid emulsions were converted into shear-thinning emulsion gels by homogenization at pressures above 250 MPa. Hydrophobic interactions between proteins were supposed to cause the gel-like network structure of emulsions.

[1]  L. W. Phipps,et al.  Factors affecting the fat globule sizes during the homogenization of milk and cream , 1964, Journal of Dairy Research.

[2]  Tomohiko Mori,et al.  Gelation Mechanism of Soybean 11S Globulin: Formation of Soluble Aggregates as Transient Intermediates , 1982 .

[3]  E. Molina,et al.  Emulsifying properties of high pressure treated soy protein isolate and 7S and 11S globulins , 2001 .

[4]  L. W. Phipps Mechanism of Oil Droplet Fragmentation in High Pressure Homogenizers , 1971, Nature.

[5]  E. Dickinson,et al.  High Pressure Effects on Emulsifying Behavior of Whey Protein Concentrate , 1995 .

[6]  J. Floury,et al.  Effect of high pressure homogenisation on methylcellulose as food emulsifier , 2003 .

[7]  Juliane Floury,et al.  Effect of high-pressure homogenization on droplet size distributions and rheological properties of model oil-in-water emulsions , 2000 .

[8]  E. Dumay,et al.  Effects of High Pressure on the Physico-chemical Characteristics of Dairy Creams and Model Oil/Water Emulsions , 1996 .

[9]  E. Dickinson Food colloids — An overview , 1989 .

[10]  J. Floury,et al.  Degradation of methylcellulose during ultra-high pressure homogenisation , 2002 .

[11]  P. Paquin,et al.  Effect of dynamic high pressure on the secondary structure of β-lactoglobulin and on its conformational properties as determined by Fourier transform infrared spectroscopy , 1998 .

[12]  A. Desrumaux,et al.  Formation of sunflower oil emulsions stabilized by whey proteins with high-pressure homogenization (up to 350 MPa): effect of pressure on emulsion characteristics , 2002 .

[13]  A. Hermansson Aggregation and Denaturation Involved in Gel Formation , 1979 .

[14]  E. Dickinson,et al.  Effect of High-Pressure Treatment of Protein on the Rheology of Flocculated Emulsions Containing Protein and Polysaccharide , 1996 .

[15]  Rekha S. Singhal,et al.  Basmati rice: a review , 2002 .

[16]  E. Dumay,et al.  High-Pressure Unfolding and Aggregation of .beta.-Lactoglobulin and the Baroprotective Effects of Sucrose , 1994 .

[17]  R. López-Fandiño,et al.  Use of high-pressure-treated milk for the production of reduced-fat cheese , 2000 .

[18]  D. Mcclements,et al.  Influence of sodium dodecyl sulfate on the physicochemical properties of whey protein-stabilized emulsions , 2000 .

[19]  P. Walstra,et al.  Making Emulsions and Foams: An Overview , 1997 .

[20]  E. Dickinson,et al.  Effect of high pressure on the emulsifying behaviour of β-lactoglobulin , 1996 .

[21]  G. Moates,et al.  Creaming and oscillation rheology of weakly flocculated concentrated emulsions , 2001 .

[22]  Tomohiko Mori,et al.  Network structure formation in thermally-induced gelation of glycinin , 1984 .

[23]  M. Britten,et al.  Effect of High-Pressure Homogenization on a Sterilized Infant Formula: Microstructure and Age Gelation , 1990 .

[24]  P. Walstra,et al.  Chapter 2:Emulsion Formation , 1998 .

[25]  S. Damodaran,et al.  Food Proteins and Their Applications , 1997 .

[26]  Chung C. Hsu,et al.  Effect of high shear on proteins , 2000, Biotechnology and bioengineering.

[27]  P. Paquin Technological properties of high pressure homogenizers: the effect of fat globules, milk proteins, and polysaccharides , 1999 .