Acid polysaccharides in the skeletal matrix and calicoblastic epithelium of the stony coral Mycetophyllia reesi.

Like many corals the skeletal organic matrix and associated epithelium of Mycetophyllia reesi is physico-chemically unstable to preparative procedures for electron microscopy. Ethanol cryofracture of mineralized and demineralized material is accompanied by delamination of tissue and skeleton. Filamentous algae occur in the interface and account for some but not all of the separation artifact. Transmission microscopy accompanied by decalcification requires embedment in glycerol jelly to preserve the skeletal organic matrix. Even then, the matrix is not fixed and is not retained within the gel using standard double fixation with or without tannic acid as an additive. Ruthenium red, in combination with osmium, prevents the matrix from physical disruption, although positional artifacts relative to the calicoblastic epithelium are still evident. Inclusion of other glycan precipitating agents in the fixative sequence (Alcian blue, iron diamine or the detergent cetylpyridinium chloride) are more useful in preserving an acid polysaccharide-rich, fibrillar, extracellular matrix after demineralization. This material is not observed in SEM preparations. The calicoblast cells appear to be the source of this extracellular material that also appears to contribute to the composition of the mineralizing matrix. Moreover, a hyaluronan-like substance appears to play a significant role in matrix structure as suggested by its degradation by hyaluronidase.

[1]  Jaubert,et al.  Organic matrix synthesis in the scleractinian coral stylophora pistillata: role in biomineralization and potential target of the organotin tributyltin , 1998, The Journal of experimental biology.

[2]  J. Marxen,et al.  Carbohydrates of the Organic Shell Matrix and the Shell-Forming Tissue of the Snail Biomphalaria glabrata (Say). , 1998, The Biological bulletin.

[3]  X. H. Wu,et al.  Control of crystal phase switching and orientation by soluble mollusc-shell proteins , 1996, Nature.

[4]  H. Yamashiro,et al.  New type of organic matrix in cirals formed at the decalcified site: Structure and composition , 1996 .

[5]  S. Weiner,et al.  Control of Aragonite or Calcite Polymorphism by Mollusk Shell Macromolecules , 1996, Science.

[6]  N. Hotchin,et al.  Cell biology of extracellular matrix, 2nd edn: edited by Elizabeth D. Hay, Plenum, 1991. £65.00 (xvii + 468 pages) ISBN 0 306 43951 4 , 1992 .

[7]  E. Bonucci.  Calcification in Biological Systems , 1992 .

[8]  D. Septier,et al.  Visualization of glycosaminoglycans in rat incisor predentin and dentin with cetylpyridinium chloride-glutaraldehyde as fixative. , 1990, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[9]  M. L. Tissier,et al.  The ultrastructure of the skeleton and skeletogenic tissues of the temperate coral Caryophyllia smithii , 1990, Journal of the Marine Biological Association of the United Kingdom.

[10]  S. Weiner,et al.  Acidic macromolecules associated with the mineral phase of scleractinian coral skeletons , 1988 .

[11]  R. Mecham,et al.  Biology of Proteoglycans , 1987 .

[12]  Y. Isa.  An electron microscope study on the mineralization of the skeleton of the staghorn coral Acropora hebes , 1986 .

[13]  S. Weiner,et al.  Interactions between acidic proteins and crystals: stereochemical requirements in biomineralization. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[14]  S. Weiner.  Organization of Organic Matrix Components in Mineralized Tissues , 1984 .

[15]  Douglas C. Wilson,et al.  Ionotropic Nucleation of Calcium Carbonate by Molluscan Matrix , 1984 .

[16]  Steve Weiner,et al.  Macromolecules in mollusc shells and their functions in biomineralization , 1984 .

[17]  E. Hunziker,et al.  Cartilage ultrastructure after high pressure freezing, freeze substitution, and low temperature embedding. II. Intercellular matrix ultrastructure - preservation of proteoglycans in their native state , 1984, The Journal of cell biology.

[18]  E. de Jong,et al.  Polysaccharide localization, coccolith formation, and Golgi dynamics in the coccolithophorid Hymenomonas carterae. , 1983, Journal of ultrastructure research.

[19]  H. Lowenstam,et al.  Minerals formed by organisms. , 1981, Science.

[20]  J. Vandermeulen.  Studies on reef corals. III. Fine structural changes of calicoblast cells in Pocillopora damicornis during settling and calcification , 1975 .

[21]  J. Vandermeulen.  Studies on reef corals. II. Fine structure of planktonic planula larva of Pocillopora damicornis, with emphasis on the aboral epidermis , 1974 .

[22]  S. D. Young.  Organic material from scleractinian coral skeletons—I. Variation in composition between several species☆ , 1971 .

[23]  O. Behnke,et al.  Preservation of intercellular substances by the cationic dye alcian blue in preparative procedures for electron microscopy. , 1970, Journal of ultrastructure research.

[24]  A. Rambourg,et al.  AN IMPROVED SILVER METHENAMINE TECHNIQUE FOR THE DETECTION OF PERIODIC ACID-REACTIVE COMPLEX CARBOHYDRATES WITH THE ELECTRON MICROSCOPE , 1967, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[25]  S. Spicer.  DIAMINE METHODS FOR DIFFERENTIATING MUCOSUBSTANCES HISTOCHEMICALLY , 1965 .

[26]  S. Wainwright.  Skeletal organization in the coral, Pocillopora damicornis , 1963 .

[27]  T. Goreau.  Histochemistry of Mucopolysaccharide-Like Substances and Alkaline Phosphatase in Madreporaria , 1956, Nature.

[28]  D. Allemand,et al.  Morphology of coral desmocytes, cells that anchor the calicoblastic epithelium to the skeleton , 1997, Coral Reefs.

[29]  B. Toole.  Proteoglycans and Hyaluronan in Morphogenesis and Differentiation , 1991 .

[30]  S. Weiner,et al.  Acidic macromolecules of mineralized tissues: the controllers of crystal formation. , 1991, Trends in biochemical sciences.

[31]  G. Hunter.  Role of proteoglycan in the provisional calcification of cartilage. A review and reinterpretation. , 1991, Clinical orthopaedics and related research.

[32]  J. G. Carter.  Skeletal biomineralization : patterns, processes, and evolutionary trends , 1991 .

[33]  E. Bonucci,et al.  Ultrastructure of Skeletal Tissues , 1990, Electron Microscopy in Biology and Medicine.

[34]  M. Takagi.  Ultrastructural cytochemistry of cartilage proteoglycans and their relation to the calcification process , 1990 .

[35]  K. Simkiss,et al.  Biomineralization : cell biology and mineral deposition , 1989 .

[36]  Y. Benayahu,et al.  Spicule formation in the gorgonian coral Pseudoplexaura flagellosa. I: Demonstration of intracellular and extracellular growth and the effect of ruthenium red during decalcification , 1987 .

[37]  A. Poole.  Proteoglycans, Chondrocalcin, and the Calcification of Cartilage Matrix in Endochondrial Ossification , 1987 .

[38]  G. Hunter,et al.  An ion-exchange mechanism of cartilage calcification. , 1987, Connective tissue research.

[39]  E. Hunziker,et al.  Structural Organization of Proteoglycans in Cartilage , 1987 .

[40]  P. Westbroek,et al.  Biomineralization and Biological Metal Accumulation , 1983 .

[41]  H. Nakahara.  Calcification of Gastropod Nacre , 1983 .

[42]  I. Johnston.  The Ultrastructure of Skeletogenesis in Hermatypic Corals , 1980 .

[43]  C. Jeuniaux,et al.  Distribution et importance quantitative de la chitine dans les coquilles de mollusques , 1979 .

[44]  A. Fontaine,et al.  A decalification method for ultrastructure of echinoderm tissues. , 1975, Stain technology.

[45]  S. Kawaguti,et al.  Electron microscopy on the polyp of staghorn corals with special reference to its skeleton formation , 1968 .