Intercellular transportation of quantum dots mediated by membrane nanotubes.

In this work, we reported that the quantum dot (QD) nanoparticles could be actively transported in the membrane nanotubes between cardiac myocytes. Single particle imaging and tracking of QDs revealed that most QDs moved in a bidirectional mode along the membrane nanotubes with a mean velocity of 1.23 mum/s. The results suggested that QDs moving in the nanotubes were coordinately motivated by molecular motors. It provides new information for the study of intercellular transportation of nanoparticles.

[1]  A. Marcus,et al.  Imaging and tracking of tat peptide-conjugated quantum dots in living cells: new insights into nanoparticle uptake, intracellular transport, and vesicle shedding. , 2007, Journal of the American Chemical Society.

[2]  Yohanns Bellaiche,et al.  Tracking individual kinesin motors in living cells using single quantum-dot imaging. , 2006, Nano letters.

[3]  Q. Sattentau,et al.  Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission , 2008, Nature Cell Biology.

[4]  Y. Toyoshima,et al.  A Single-headed Recombinant Fragment of Dictyostelium Cytoplasmic Dynein Can Drive the Robust Sliding of Microtubules* , 2004, Journal of Biological Chemistry.

[5]  T. Schroer,et al.  Dynactin increases the processivity of the cytoplasmic dynein motor , 1999, Nature Cell Biology.

[6]  Shuming Nie,et al.  Cell-penetrating quantum dots based on multivalent and endosome-disrupting surface coatings. , 2007, Journal of the American Chemical Society.

[7]  Ye Guang Chen,et al.  Lateral diffusion of TGF-β type I receptor studied by single-molecule imaging , 2007 .

[8]  Sangeeta N. Bhatia,et al.  The European charter for counteracting obesity: A late but important step towards action. Observations on the WHO-Europe ministerial conference, Istanbul, November 15–17, 2006 , 2007, The international journal of behavioral nutrition and physical activity.

[9]  N. Hirokawa,et al.  A processive single-headed motor: kinesin superfamily protein KIF1A. , 1999, Science.

[10]  Hong Ding,et al.  Imaging pancreatic cancer using bioconjugated InP quantum dots. , 2009, ACS nano.

[11]  Nicolas Chenouard,et al.  Prions hijack tunnelling nanotubes for intercellular spread , 2009, Nature Cell Biology.

[12]  Simon C Watkins,et al.  Functional connectivity between immune cells mediated by tunneling nanotubules. , 2005, Immunity.

[13]  M. Purbhoo,et al.  Long-Distance Calls Between Cells Connected by Tunneling Nanotubules , 2005, Science's STKE.

[14]  H. Gerdes,et al.  Tunneling nanotubes: A new route for the exchange of components between animal cells , 2007, FEBS letters.

[15]  K. O'Connor,et al.  Vesicle traffic through intercellular bridges in DU 145 human prostate cancer cells , 2004, Journal of cellular and molecular medicine.

[16]  W. Mothes,et al.  Retroviruses can establish filopodial bridges for efficient cell-to-cell transmission , 2007, Nature Cell Biology.

[17]  Eric F. Wieschaus,et al.  Coordination of opposite-polarity microtubule motors , 2002, The Journal of cell biology.

[18]  Hans-Hermann Gerdes,et al.  Nanotubular Highways for Intercellular Organelle Transport , 2004, Science.

[19]  H. Takematsu,et al.  Wheat germ agglutinin stains dispersed post‐golgi vesicles after treatment with the cytokinesis inhibitor psychosine , 2008, Journal of cellular physiology.

[20]  Melanie J. I. Müller,et al.  Tug-of-war as a cooperative mechanism for bidirectional cargo transport by molecular motors , 2008, Proceedings of the National Academy of Sciences.

[21]  Paul G. McMenamin,et al.  Cutting Edge: Membrane Nanotubes In Vivo: A Feature of MHC Class II+ Cells in the Mouse Cornea1 , 2008, The Journal of Immunology.

[22]  Yuliang Zhang,et al.  Transmembrane delivery of the cell-penetrating peptide conjugated semiconductor quantum dots. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[23]  Jayanth Panyam,et al.  Dynamics of Endocytosis and Exocytosis of Poly(D,L-Lactide-co-Glycolide) Nanoparticles in Vascular Smooth Muscle Cells , 2003, Pharmaceutical Research.

[24]  S. Kimura,et al.  M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex , 2009, Nature Cell Biology.

[25]  Paul R. Selvin,et al.  Kinesin and Dynein Move a Peroxisome in Vivo: A Tug-of-War or Coordinated Movement? , 2005, Science.

[26]  T. Vu,et al.  Quantum dots monitor TrkA receptor dynamics in the interior of neural PC12 cells. , 2006, Nano letters.

[27]  M. Bruchez,et al.  Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots , 2003, Nature Biotechnology.

[28]  Philippe Rostaing,et al.  Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking , 2003, Science.

[29]  M. Welte,et al.  Bidirectional Transport along Microtubules , 2004, Current Biology.

[30]  Shuming Nie,et al.  Bioconjugated quantum dots for in vivo molecular and cellular imaging. , 2008, Advanced drug delivery reviews.

[31]  Shuming Nie,et al.  Proton-sponge coated quantum dots for siRNA delivery and intracellular imaging. , 2008, Journal of the American Chemical Society.

[32]  Do Won Hwang,et al.  In vitro derby imaging of cancer biomarkers using quantum dots. , 2009, Small.

[33]  Sandra J Rosenthal,et al.  Binding of muscimol-conjugated quantum dots to GABAC receptors. , 2005, Journal of the American Chemical Society.

[34]  Xiaolin Nan,et al.  Observation of individual microtubule motor steps in living cells with endocytosed quantum dots. , 2005, The journal of physical chemistry. B.

[35]  G. Sukhikh,et al.  Cell-to-cell cross-talk between mesenchymal stem cells and cardiomyocytes in co-culture , 2007, Journal of cellular and molecular medicine.

[36]  R. Brandes,et al.  Cell-to-Cell Connection of Endothelial Progenitor Cells With Cardiac Myocytes by Nanotubes: A Novel Mechanism for Cell Fate Changes? , 2005, Circulation research.

[37]  D. Davis,et al.  Membrane nanotubes: dynamic long-distance connections between animal cells , 2008, Nature Reviews Molecular Cell Biology.

[38]  J. Caviston,et al.  Microtubule motors at the intersection of trafficking and transport. , 2006, Trends in cell biology.

[39]  M. Howarth,et al.  Targeting quantum dots to surface proteins in living cells with biotin ligase. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[40]  Shuming Nie,et al.  Quantum dots for in vivo molecular and cellular imaging. , 2007, Methods in molecular biology.

[41]  M. Neil,et al.  Structurally Distinct Membrane Nanotubes between Human Macrophages Support Long-Distance Vesicular Traffic or Surfing of Bacteria1 , 2006, The Journal of Immunology.

[42]  H. Gerdes,et al.  Intercellular transfer mediated by tunneling nanotubes. , 2008, Current opinion in cell biology.