Accelerated Neutrophil Apoptosis in Mice Lacking A1-a, a Subtype of the bcl-2–related A1 Gene

To elucidate the role of A1, a new member of the Bcl-2 family of apoptosis regulators active in hematopoietic cell apoptosis, we established mice lacking A1-a, a subtype of the A1 gene in mice (A1-a−/− mice). Spontaneous apoptosis of peripheral blood neutrophils of A1-a−/− mice was enhanced compared with that of either wild-type mice or heterozygous mutants (A1-a+/− mice). Neutrophil apoptosis inhibition induced by lipopolysaccharide treatment in vitro or transendothelial migration in vivo observed in wild-type mice was abolished in both A1-a−/− and A1-a+/− animals. On the other hand, the extent of tumor necrosis factor α–induced acceleration of neutrophil apoptosis did not differ among A1-a−/−, A1-a+/−, and wild-type mice. The descending order of A1 mRNA expression was wild-type, A1-a+/−, and A1-a−/−. Taken together, these results suggest that A1 is involved in inhibition of certain types of neutrophil apoptosis.

[1]  D. Loh,et al.  Multiple gene duplication and expression of mouse bcl-2-related genes, A1. , 1998, International immunology.

[2]  R. Bitar,et al.  Granulocytic differentiation of HL‐60 cells results in spontaneous apoptosis mediated by increased caspase expression , 1997, FEBS letters.

[3]  K. Ragheb,et al.  Defects in intracellular oxidative metabolism of neutrophils undergoing apoptosis , 1997, Journal of leukocyte biology.

[4]  T. Miyawaki,et al.  Involvement of reactive oxygen intermediates in spontaneous and CD95 (Fas/APO-1)-mediated apoptosis of neutrophils. , 1997, Blood.

[5]  M. Nehls,et al.  Suppression of Apoptosis by Nitric Oxide via Inhibition of Interleukin-1β–converting Enzyme (ICE)-like and Cysteine Protease Protein (CPP)-32–like Proteases , 1997, The Journal of experimental medicine.

[6]  J. Marshall,et al.  Neutrophil apoptosis is modulated by endothelial transmigration and adhesion molecule engagement. , 1997, Journal of immunology.

[7]  G. Núñez,et al.  A1 demonstrates restricted tissue distribution during embryonic development and functions to protect against cell death. , 1996, The American journal of pathology.

[8]  A. Karsan,et al.  Endothelial Cell Death Induced by Tumor Necrosis Factor-α Is Inhibited by the Bcl-2 Family Member, A1* , 1996, The Journal of Biological Chemistry.

[9]  P. Kiener,et al.  Differential expression of Fas (CD95) and Fas ligand on normal human phagocytes: implications for the regulation of apoptosis in neutrophils , 1996, The Journal of experimental medicine.

[10]  A. Karsan,et al.  Cloning of human Bcl-2 homologue: inflammatory cytokines induce human A1 in cultured endothelial cells. , 1996, Blood.

[11]  John Calvin Reed,et al.  A1, a Bcl-2 family member, prolongs cell survival and permits myeloid differentiation. , 1996, Blood.

[12]  M. Jäättelä,et al.  A20 zinc finger protein inhibits TNF and IL-1 signaling. , 1996, Journal of immunology.

[13]  J. Larrick,et al.  Biochemical pathways of apoptosis: nicotinamide adenine dinucleotide- deficient cells are resistant to tumor necrosis factor or ultraviolet light activation of the 24-kD apoptotic protease and DNA fragmentation , 1996, The Journal of experimental medicine.

[14]  H. Watanabe,et al.  Rapid killing of human neutrophils by the potent activator phorbol 12‐myristate 13‐acetate (PMA) accompanied by changes different from typical apoptosis or necrosis , 1996, Journal of leukocyte biology.

[15]  John Calvin Reed,et al.  Immunoblot analysis of cellular expression of Bcl-2 family proteins, Bcl-2, Bax, Bcl-X and Mcl-1, in human peripheral blood and lymphoid tissues. , 1995, International immunology.

[16]  D. Goeddel,et al.  TRAF2-mediated activation of NF-kappa B by TNF receptor 2 and CD40 , 1995, Science.

[17]  Y. Takeda,et al.  Inhibition by Bacterial Lipopolysaccharide of Spontaneous and TNF‐α‐Induced Human Neutrophil Apoptosis In Vitro , 1995, Microbiology and immunology.

[18]  D. Goeddel,et al.  The TNF receptor 1-associated protein TRADD signals cell death and NF-κB activation , 1995, Cell.

[19]  S. Nagata,et al.  Involvement of an ICE-like protease in Fas-mediated apoptosis , 1995, Nature.

[20]  H. Shinzawa,et al.  In vivo regulation of rat neutrophil apoptosis occurring spontaneously or induced with TNF-alpha or cycloheximide. , 1995, Journal of immunology.

[21]  S. Korsmeyer,et al.  Bad, a heterodimeric partner for Bcl-xL and Bcl-2, displaces bax and promotes cell death , 1995, Cell.

[22]  R. Craig,et al.  Mcl-1, a member of the Bcl-2 family, delays apoptosis induced by c-Myc overexpression in Chinese hamster ovary cells. , 1994, Cancer research.

[23]  K. Schulze-Osthoff,et al.  Divergent signalling via APO‐1/Fas and the TNF receptor, two homologous molecules involved in physiological cell death. , 1994, The EMBO journal.

[24]  D. Goeddel,et al.  A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor , 1994, Cell.

[25]  C. Thompson,et al.  Identification of immunosuppressant-induced apoptosis in a murine B-cell line and its prevention by bcl-x but not bcl-2. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[26]  D. Loh,et al.  Targeted disruption of Bcl-2 alpha beta in mice: occurrence of gray hair, polycystic kidney disease, and lymphocytopenia. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[27]  M. Cleary,et al.  Bcl2 inhibits apoptosis associated with terminal differentiation of HL-60 myeloid leukemia cells. , 1994, Blood.

[28]  I. Weissman,et al.  bcl-2 inhibits apoptosis of neutrophils but not their engulfment by macrophages , 1994, The Journal of experimental medicine.

[29]  H. Nakauchi,et al.  Requirement for CD8 beta chain in positive selection of CD8-lineage T cells. , 1994, Science.

[30]  B. Calnan,et al.  Requirement for the orphan steroid receptor Nur77 in apoptosis of T-cell hybridomas , 1994, Nature.

[31]  S. Grant,et al.  Induction of apoptotic DNA damage and cell death by activation of the sphingomyelin pathway. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[32]  S. Korsmeyer,et al.  Bcl-2-deficient mice demonstrate fulminant lymphoid apoptosis, polycystic kidneys, and hypopigmented hair , 1993, Cell.

[33]  C. Haslett,et al.  Inhibition of apoptosis and prolongation of neutrophil functional longevity by inflammatory mediators , 1993, Journal of leukocyte biology.

[34]  V. Stewart,et al.  Disappearance of the lymphoid system in Bcl-2 homozygous mutant chimeric mice. , 1993, Science.

[35]  S. Korsmeyer,et al.  Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death , 1993, Cell.

[36]  C. Thompson,et al.  bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death , 1993, Cell.

[37]  M. Berger,et al.  Characterization of A1, a novel hemopoietic-specific early-response gene with sequence similarity to bcl-2. , 1993, Journal of immunology.

[38]  S. Yonehara,et al.  Rapid acceleration of neutrophil apoptosis by tumor necrosis factor-alpha. , 1993, International immunology.

[39]  Scott W. Lowe,et al.  p53 is required for radiation-induced apoptosis in mouse thymocytes , 1993, Nature.

[40]  R. Craig,et al.  MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[41]  Y. Hannun,et al.  Programmed cell death induced by ceramide. , 1993, Science.

[42]  F. Herrmann,et al.  Prolongation of survival of human polymorphonuclear neutrophils by granulocyte-macrophage colony-stimulating factor is caused by inhibition of programmed cell death. , 1992, Blood.

[43]  A. Mantovani,et al.  Modulation of granulocyte survival and programmed cell death by cytokines and bacterial products. , 1992, Blood.

[44]  D. Green,et al.  Role for c-myc in activation-induced apoptotic cell death in T cell hybridomas. , 1992, Science.

[45]  M. Pawlita,et al.  Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. Sequence identity with the Fas antigen. , 1992, The Journal of biological chemistry.

[46]  M. Pierotti,et al.  bcl-2 proto-oncogene expression in normal and neoplastic human myeloid cells. , 1992, Blood.

[47]  Atsushi Hase,et al.  The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis , 1991, Cell.

[48]  S. Korsmeyer,et al.  Deregulated Bcl-2 gene expression selectively prolongs survival of growth factor-deprived hemopoietic cell lines. , 1990, Journal of immunology.

[49]  N. Hogg,et al.  Vitronectin receptor-mediated phagocytosis of cells undergoing apoptosis , 1990, Nature.

[50]  David L. Vaux,et al.  Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells , 1988, Nature.

[51]  G. Lazarus,et al.  Labeling of peripheral blood polymorphonuclear leukocytes with indium-111: a new method for the quantitation of in-vivo accumulation of PMNLs in rabbit skin. , 1984, The Journal of investigative dermatology.