Accumulation of (3H)glycine by cone bipolar neurons in the cat retina

Cone bipolar neurons in the cat retina were studied in serial sections prepared as electron microscope autoradiograms following intravitreal injection of (3H)glycine. The goal was to learn whether the cone bipolar types that accumulate glycine correspond to the types thought on other grounds to be inhibitory. About half of the cone bipolars in a given patch of retina showed specific accumulation of silver grains. The specificity of accumulation was similar to that shown by glycine‐accumulating amacrines. All of the cone bipolars arborizing in sublamina b accumulated glycine but none of the cone bipolars arborizing in sublamina a did so. The types of cone bipolars accumulating glycine did not match the types thought to be inhibitory. Cone bipolar types CBb1 and CBb2 both form gap junctions with the glycine‐accumulating AII amacrine, thus raising the possibility that glycine might accumulate in these cone bipolars by diffusion from the AII cell or vice versa. Thus it is logically impossible to tell which of these three cells contains a high‐affinity uptake mechanism for glycine and consequently which of the three might actually use glycine as a neurotransmitter.

[1]  P Sterling,et al.  Four types of amacrine in the cat retina that accumulate GABA , 1983, The Journal of comparative neurology.

[2]  M. Slaughter,et al.  Physiological and pharmacological basis of GABA and glycine action on neurons of mudpuppy retina. II. Amacrine and ganglion cells. , 1981, Journal of neurophysiology.

[3]  D. Lam,et al.  The uptake and release of [3H]glycine in the goldfish retina. , 1980, The Journal of physiology.

[4]  R. Pourcho Uptake of [3H]glycine and [3H]GABA by amacrine cells in the cat retina , 1980, Brain Research.

[5]  M. Voaden,et al.  The uptake of [3]Hγ-amino butyric acid and [3H]glycine by the isolated retina of the frog , 1974 .

[6]  P. Mandel,et al.  Free amino acids of chicken and rat retina. , 1972, Brain research.

[7]  H. Saito Pharmacological and morphological differences between X- and Y-type ganglion cells in the cat's retina , 1983, Vision Research.

[8]  R. Pourcho,et al.  A combined golgi and autoradiographic study of (3H)glycine‐accumulating amacrine cells in the cat retina , 1985, The Journal of comparative neurology.

[9]  H. Wässle,et al.  Action and localization of glycine and taurine in the cat retina. , 1985, The Journal of physiology.

[10]  B. Ehinger,et al.  Light-evoked release of glycine from cat and rabbit retina , 1976, Brain Research.

[11]  Peter Sterling,et al.  Preparing autoradiograms of serial sections for electron microscopy , 1979, Journal of Neuroscience Methods.

[12]  M. Slaughter,et al.  Physiological and pharmacological basis of GABA and glycine action on neurons of mudpuppy retina. I. Receptors, horizontal cells, bipolars, and G-cells. , 1981, Journal of Neurophysiology.

[13]  P. Sterling,et al.  Microcircuitry of beta ganglion cells in cat retina , 1986, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[14]  P Sterling,et al.  Microcircuitry of bipolar cells in cat retina , 1984, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[15]  R. Marc,et al.  Glycinergic pathways in the goldfish retina , 1981, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[16]  P. Sterling Microcircuitry of the cat retina. , 1983, Annual review of neuroscience.