Continuous flocculation-sedimentation for harvesting Nannochloropsis salina biomass.

A continuous flow process is developed for recovery of the biomass of the marine microalga Nannochloropsis salina. Flocculation-sedimentation is used to recover the biomass from an algal suspension with an initial dry biomass concentration of 0.5 g L(-1), as would be typical of a raceway-based biomass production system. More than 85% of the biomass initially in suspension could be settled by gravity in a flocculation-sedimentation device with a total residence time of ∼148 min. Aluminum sulfate was used as an inexpensive, readily available and safe flocculant. The optimal flocculant dosage (as Al2(SO4)3) was 229 mg L(-1). Relative to a highly effective 62-min batch flocculation-sedimentation process for the same alga and flocculant, the continuous flow operation took longer and required nearly double the flocculant dose. The design of the flocculation-sedimentation system is explained.

[1]  Michael K. Danquah,et al.  Dewatering of microalgal culture for biodiesel production: exploring polymer flocculation and tangential flow filtration , 2009 .

[2]  Tawan Chatsungnoen,et al.  An assessment of inexpensive methods for recovery of microalgal biomass and oils : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biotechnology at Massey University, Palmerston North, New Zealand , 2015 .

[3]  Andrea J Garzon-Sanabria,et al.  Harvesting Nannochloris oculata by inorganic electrolyte flocculation: effect of initial cell density, ionic strength, coagulant dosage, and media pH. , 2012, Bioresource technology.

[4]  L. Rodolfi,et al.  Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor , 2009, Biotechnology and bioengineering.

[5]  D. Bilanović,et al.  Flocculation of microalgae in brackish and sea waters , 1988 .

[6]  N. De Pauw,et al.  Potential of electrolytic flocculation for recovery of micro-algae , 1997 .

[7]  H. Stechemesser,et al.  Coagulation and flocculation. , 2005 .

[8]  Y. Chisti,et al.  Forward osmosis with waste glycerol for concentrating microalgae slurries , 2015 .

[9]  Jo‐Shu Chang,et al.  Microalgae biomass harvesting by bioflocculation-interpretation by classical DLVO theory , 2015 .

[10]  Y. Chisti,et al.  Recovery of microalgal biomass and metabolites: process options and economics. , 2003, Biotechnology advances.

[11]  John Bratby,et al.  Coagulation and Flocculation in Water and Wastewater Treatment , 2008 .

[12]  Thongchai Srinophakun,et al.  Design of raceway ponds for producing microalgae , 2012 .

[13]  Yoon,et al.  Effects of harvesting method and growth stage on the flocculation of the green alga Botryococcus braunii , 1998 .

[14]  Michael A. Borowitzka,et al.  Culturing microalgae in outdoor ponds , 2005 .

[15]  K. Reardon,et al.  Conversion of lipid-extracted Nannochloropsis salina biomass into fermentable sugars , 2015 .

[16]  G. Fogg,et al.  Algal cultures and phytoplankton ecology , 1966 .

[17]  C. C. Lee,et al.  Water and Wastewater Calculations Manual , 2001 .

[18]  A. Shariati,et al.  Using nano-chitosan for harvesting microalga Nannochloropsis sp. , 2013, Bioresource technology.

[19]  Michael L. Gerardo,et al.  Harvesting of microalgae within a biorefinery approach: A review of the developments and case studies from pilot-plants , 2015 .

[20]  Yusuf Chisti,et al.  Raceways-based Production of Algal Crude Oil , 2013 .

[21]  P. Harrison,et al.  Recipes for Freshwater and Seawater Media , 2005 .

[22]  Andrew Hoadley,et al.  Dewatering of microalgal cultures : a major bottleneck to algae-based fuels , 2010 .

[23]  D. Vandamme,et al.  Flocculation as a low-cost method for harvesting microalgae for bulk biomass production. , 2013, Trends in biotechnology.

[24]  S. Einbinder,et al.  Inexpensive non-toxic flocculation of microalgae contradicts theories; overcoming a major hurdle to bulk algal production. , 2012, Biotechnology advances.

[25]  Jay R. Werber,et al.  Application of membrane dewatering for algal biofuel , 2015 .

[26]  Cun-wen Wang,et al.  Optimal conditions of different flocculation methods for harvesting Scenedesmus sp. cultivated in an open-pond system. , 2013, Bioresource technology.

[27]  Jeffrey Philip Obbard,et al.  Screening of marine microalgae for biodiesel feedstock , 2011 .

[28]  R. Riffat Fundamentals of Wastewater Treatment and Engineering , 2012 .

[29]  Peter J. Ashman,et al.  Microbial flocculation, a potentially low-cost harvesting technique for marine microalgae for the production of biodiesel , 2009, Journal of Applied Phycology.

[30]  R. Hogg,et al.  Flocculation and dewatering , 2000 .

[31]  Peter J. Ashman,et al.  Harvesting of marine microalgae by electroflocculation: The energetics, plant design, and economics , 2013 .

[32]  Gary B. Tatterson,et al.  Mechanically Stirred Vessels , 2004 .

[33]  Xxyyzz,et al.  Design of Municipal Wastewater Treatment Plants , 2010 .

[34]  Jinyue Yan,et al.  Energy from algae: Current status and future trends: Algal biofuels – A status report , 2011 .

[35]  Hui Shen,et al.  Basic Culturing Techniques , 2007 .

[36]  David W. Hendricks,et al.  Water Treatment Unit Processes , 2006 .

[37]  Yusuf Chisti,et al.  Fuels from microalgae , 2010 .

[38]  M. Rebhun Floc Formation and Breakup in Continuous Flow Flocculation and in Contact Filtration , 1990 .

[39]  G. L. Karia,et al.  Wastewater Treatment: Concepts and Design Approach , 2013 .

[40]  Aikaterini Papazi,et al.  Harvesting Chlorella minutissima using cell coagulants , 2010, Journal of Applied Phycology.

[41]  J. Gregory,et al.  Coagulation by hydrolysing metal salts , 2003 .

[42]  R. Hogg,et al.  Aggregate size distributions in flocculation , 2001 .

[43]  Yusuf Chisti,et al.  Harvesting microalgae by flocculation–sedimentation , 2016 .

[44]  Michael K. Danquah,et al.  Microalgal growth characteristics and subsequent influence on dewatering efficiency , 2009 .

[45]  R. Viadero Sedimentation and Flotation , 2005 .

[46]  Joan Salvadó,et al.  Harvesting the microalgae Phaeodactylum tricornutum with polyaluminum chloride, aluminium sulphate, chitosan and alkalinity-induced flocculation , 2011, Journal of Applied Phycology.

[47]  Yusuf Chisti,et al.  Constraints to commercialization of algal fuels. , 2013, Journal of biotechnology.

[48]  James Y. Oldshue,et al.  Fluid Mixing Technology , 1983 .

[49]  K. Niyogi,et al.  High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. , 2011, Proceedings of the National Academy of Sciences.