Bovine Rotavirus V1005a P5,Nota P12,Typelike AllViruses ina GermanSurvey

Bovine rotavirus (BRV)V1005, like 34further cell culture-adapted strains ina 6-year surveyinUpper Bavaria, Germany, isnota P12buta P5P-type rotavirus. Theconclusion isbased on dotblothybridization withP1-, P5-, andPll-specific cDNAs,encompassing theVP8*region ofmajor sequencediversity, andon PCR usingP1-,P5-,andP11-specific primerpairsderived fromtheVP5*region ofVP4(VP5*andVP8*, respectively, arethelarger andsmaller tryptic cleavage products ofVP4).Sequencing ofthehyperdivergent region ofVP4confirmed theclose relatedness ofBRVV1005toBRVUK,theP5prototype virus. GroupA bovine rotaviruses (BRVs) area majorinfectious causeofdiarrhea incalves. Bothoutercapsid rotaviral proteins, VP4andVP7,can independently mediate active and