Three-dimensional microscopic tomographic imagings of the cataract in a human lens in vivo.

The problem of three-dimensional visualization of a human lens in vivo has been solved by a technique of volume rendering a transformed series of 60 rotated Scheimpflug (a dual slit reflected light microscope) digital images. The data set was obtained by rotating the Scheimpflug camera about the optic axis of the lens in 3 degree increments. The transformed set of optical sections were first aligned to correct for small eye movements, and then rendered into a volume reconstruction with volume rendering computer graphics techniques. To help visualize the distribution of lens opacities (cataracts) in the living, human lens the intensity of light scattering was pseudocolor coded and the cataract opacities were displayed as a movie.

[1]  A. Bron,et al.  Specular microscopy of the human lens. , 1981, Transactions of the ophthalmological societies of the United Kingdom.

[2]  R. Weale,et al.  The Eye and Aging , 1978 .

[3]  V. Dragomirescu,et al.  Development of a New Equipment for Rotating Slit Image Photography According to Scheimpflug’s Principle , 1978 .

[4]  Marc Levoy,et al.  Display of surfaces from volume data , 1988, IEEE Computer Graphics and Applications.

[5]  B R Masters,et al.  Transformation of a set of slices rotated on a common axis to a set of Z-slices: application to three-dimensional visualization of the in vivo human lens. , 1997, Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society.

[6]  K. Sasaki,et al.  Evaluating Cataract Development with the Scheimpflug Camera , 1990 .

[7]  J H Massig,et al.  Real-time confocal laser scan microscope for examination and diagnosis of the eye in vivo. , 1994, Applied optics.

[8]  Masters Br.  Three-dimensional visualization of human cataract in vivo. , 1996 .

[9]  B R Masters,et al.  Confocal microscopy of the in‐situ crystalline lens , 1992, Journal of microscopy.

[10]  B. R. Masters,et al.  Observation of the rabbit cornea and lens with a new real‐time confocal scanning optical microscope , 1990 .

[11]  B R Masters.  Three-dimensional confocal microscopy of the lens. , 1996, Ophthalmic research.

[12]  K Sasaki,et al.  Computed tomographic images and three-dimensional expression of crystalline lens findings from multiple slices of Scheimpflug slit images. , 1995, Ophthalmic research.

[13]  B. Masters.  Optical tomography of the in vivo human lens: three-dimensional visualization of cataracts. , 1996, Journal of biomedical optics.

[14]  L. Chylack,et al.  New non-contact specular microscope for lens epithelial cell visualization. , 1997, Experimental eye research.