A label-free cell separation using surface acoustic waves

We present two-stage microfluidic platform for a continuous label-free cell separation using surface acoustic waves. In the proposed platform, cells are first lined up at the center of the channel by using standing surface acoustic waves without introducing any external sheath flow. After focused at the center of the channel, the cells are then entered to the actual cell separation stage where the larger cell are exposed to more lateral displacement in the channel towards the pressure node due to the acoustic force differences. Consequently, different size cells are separated into multiple collection outlets. The focusing and separation of the cells can be accomplished simultaneously in the present two-stage microfluidic device. The device doesn't require the use of the sheath flow for positioning or aligning of cells. In this study, we demonstrated the separation of two different size particle streams (3μm and 10μm) with this microfluidic platform without introducing any external sheath flow.

[1]  Martin Heller,et al.  Multidirectional sorting modes in deterministic lateral displacement devices. , 2008, Physical review. E, Statistical, nonlinear, and soft matter physics.

[2]  Victor Steinberg,et al.  Continuous particle size separation and size sorting using ultrasound in a microchannel , 2006 .

[3]  Mehmet Toner,et al.  A microfluidic device for practical label-free CD4(+) T cell counting of HIV-infected subjects. , 2007, Lab on a chip.

[4]  K. Dholakia,et al.  Microfluidic sorting in an optical lattice , 2003, Nature.

[5]  H M Hertz,et al.  Ultrasonic standing wave manipulation technology integrated into a dielectrophoretic chip. , 2006, Lab on a chip.

[6]  D. A. Christopher,et al.  Advances in ultrasound biomicroscopy. , 2000, Ultrasound in medicine & biology.

[7]  Daniel Ahmed,et al.  Acoustic tweezers: patterning cells and microparticles using standing surface acoustic waves (SSAW). , 2009, Lab on a chip.

[8]  M.C. Wu,et al.  Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers , 2007, IEEE Journal of Selected Topics in Quantum Electronics.

[9]  Jutamaad Satayavivad,et al.  Microfluidic approaches to malaria detection. , 2004, Acta tropica.

[10]  Thomas Laurell,et al.  Particle separation using ultrasound can radically reduce embolic load to brain after cardiac surgery. , 2004, The Annals of thoracic surgery.

[11]  T. Laurell,et al.  Free flow acoustophoresis: microfluidic-based mode of particle and cell separation. , 2007, Analytical chemistry.

[12]  F. Alviano,et al.  A tag‐less method of sorting stem cells from clinical specimens and separating mesenchymal from epithelial progenitor cells , 2009, Cytometry. Part B, Clinical cytometry.

[13]  Michael T. Laub,et al.  Continuous Particle Separation Through Deterministic Lateral Displacement , 2004 .

[14]  Daniel Ahmed,et al.  Focusing microparticles in a microfluidic channel with standing surface acoustic waves (SSAW). , 2008, Lab on a chip.

[15]  D A Weitz,et al.  Surface acoustic wave actuated cell sorting (SAWACS). , 2010, Lab on a chip.

[16]  Thomas Laurell,et al.  Chip integrated strategies for acoustic separation and manipulation of cells and particles. , 2007, Chemical Society reviews.

[17]  Thomas Laurell,et al.  Separation of lipids from blood utilizing ultrasonic standing waves in microfluidic channels. , 2004, The Analyst.

[18]  M. Yamada,et al.  Hydrodynamic filtration for on-chip particle concentration and classification utilizing microfluidics. , 2005, Lab on a chip.

[19]  K. Yosioka,et al.  Acoustic radiation pressure on a compressible sphere , 1955 .

[20]  Thomas Laurell,et al.  Acoustic control of suspended particles in micro fluidic chips. , 2004, Lab on a chip.

[21]  Hsien-Chang Chang,et al.  A continuous high-throughput bioparticle sorter based on 3D traveling-wave dielectrophoresis. , 2009, Lab on a chip.

[22]  T. Huang,et al.  Continuous particle separation in a microfluidic channel via standing surface acoustic waves (SSAW). , 2009, Lab on a chip.

[23]  P. Gascoyne,et al.  Particle separation by dielectrophoresis , 2002, Electrophoresis.

[24]  Minoru Seki,et al.  Microfluidic devices for size-dependent separation of liver cells , 2007, Biomedical microdevices.

[25]  S. Takayama,et al.  Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification. , 2007, Analytical chemistry.