The analytical pyrolysis of carbohydrates. 2—differentiation of homopolyhexoses according to their linkage type, by pyrolysis–mass spectrometry and pyrolysis‐gas chromatography/mass spectrometry

Curiepoint pyrolysis (Py)-mass spectrometry (14 eV electron impact) in various modes of operation and Curiepoint pyrolysis-gas chromatograpy/mass spectrometry (Py-GC/MS) afforded discrimination between homopolyhexoses of different glycosidic linkage type and configuration: (1 2)-β-, (1 3)-β-, (1 4)-α-, (1 4)-β-, (1 6)-α, (1 6)-β-glucan and (1 2)-α-mannan. Pyrolysis-mass spectrometry in multichannel averaging mode, combined with discriminant analysis data processing, revealed significant discrimination between all polyhexoses, except for the (16) linked polymers. By using preheated telescopic glass tube sample holders, additional specificity of the pyrolysis-mass spectral fingerprint for the (1 3) linked carbohydrate was obtained. Pyrolysis-mass spectrometry in time-resolved mode yielded ion profiles, especially characteristic for the (1 3)-β-glucan. Py-GC/MS was an additional discriminating dimension (retention time), providing structural information about the pyrolysate constituents, in particular concerning the anhydrohexoses.

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