The molecular layer of the adult cat cerebellar cortex after lesion of the parallel fibers: an optic and electron microscope study.

Summary Section of parallel fibers by a sagittal cut of the adult cat cerebellar cortex results in a diminution in the number of Purkinje cell dendritic spines, seen on Golgi preparations, for a few millimeters to each side of the lesion. Some dendritic trees even appear to lose some terminal branchlets. The loss of dendritic spines has been confirmed in electrom microscopy. Three to five days after the lesion, parallel fibers and their synaptic knobs are seen to undergo the opaque type of Wallerian degeneration. A few days later, degenerating terminals are virtually absent in the tissue. This disappearance is accompanied by a diminution in the number of spines in the inter-dendritic fields. After 15 days many dendrites become densely opaque and persist in the tissue for at least as long as 60 days. A relative increase in the number of synaptic boutons is also observed at these time intervals.

[1]  D E Hillman,et al.  The primate cerebellar cortex: a Golgi and electron microscopic study. , 1967, Progress in brain research.

[2]  E. M. Larramendi,et al.  Synapses on the Purkinje cell spines in the mouse. An electronmicroscopic study. , 1967, Brain research.

[3]  J SZENTAGOTHAI,et al.  THE USE OF DEGENERATION METHODS IN THE INVESTIGATION OF SHORT NEURONAL CONNEXIONS. , 1965, Progress in brain research.

[4]  E. Reynolds.  THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY , 1963, The Journal of cell biology.

[5]  T. Powell,et al.  Some observations on transneuronal cell degeneration in the olfactory bulb of the rabbit. , 1962, Journal of anatomy.

[6]  M. Colonnier.  EXPERIMENTAL DEGENERATION IN THE CEREBRAL CORTEX. , 1964, Journal of anatomy.

[7]  Robert M. Herndon,et al.  THE FINE STRUCTURE OF THE PURKINJE CELL , 1963, The Journal of cell biology.

[8]  Gray Eg,et al.  The granule cells, mossy synapses and Purkinje spine synapses of the cerebellum: light and electron microscope observations. , 1961 .

[9]  A. Scheibel,et al.  Pattern and field in cortical structure: The rabbit , 1967, The Journal of comparative neurology.

[10]  P. Gray.  The microtomist's formulary and guide , 1954 .

[11]  M. Colonnier.  The Structural Design of the Neocortex , 1965 .

[12]  A. Scheibel,et al.  Synaptic loci on parietal cortical neurons: terminations of corpus callosum fibers. , 1967, Science.

[13]  S. Gobel.  Electron microscopical studies of the cerebellar molecular layer. , 1967, Journal of ultrastructure research.

[14]  C. R. Dutta.  The Purkinje cell dendritic branchlets and their relation with the parallel fibers Light and electron microscopic observations , 1964 .

[15]  A. Scheibel,et al.  Loss of Dendrite Spines as an Index of Pre-Synaptic Terminal Patterns , 1966, Nature.

[16]  R. Herndon.  THE FINE STRUCTURE OF THE RAT CEREBELLUM , 1964, The Journal of cell biology.

[17]  M. Colonnier.  Synaptic patterns on different cell types in the different laminae of the cat visual cortex. An electron microscope study. , 1968, Brain research.

[18]  Jones Wh,et al.  Changes in the dendritic organization of neurons in the cerebral cortex following deafferentation. , 1962 .

[19]  Gray Eg.  Axo-somatic and axo-dendritic synapses of the cerebral cortex: An electron microscope study , 1959 .

[20]  John H. Luft,et al.  IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS , 1961, The Journal of biophysical and biochemical cytology.

[21]  R. Lund,et al.  Formalin Perfusion for Correlative Light- and Electron-Microscopical Studies of the Nervous System , 1966 .