Dose response effects of 810 nm laser light on mouse primary cortical neurons

In the past four decades numerous studies have reported the efficacy of low level light (laser) therapy (LLLT) as a treatment for diverse diseases and injuries. Recent studies have shown that LLLT can biomodulate processes in the central nervous system and has been extensively studied as a stroke treatment. However there is still a lack of knowledge on the effects of LLLT at the cellular level in neurons. The present study aimed to study the effect of 810 nm laser on several cellular processes in primary cortical neurons cultured from embryonic mouse brains.

[1]  S. Michalikova,et al.  Emotional responses and memory performance of middle-aged CD1 mice in a 3D maze: Effects of low infrared light , 2008, Neurobiology of Learning and Memory.

[2]  Da Xing,et al.  High fluence low‐power laser irradiation induces mitochondrial permeability transition mediated by reactive oxygen species , 2009, Journal of cellular physiology.

[3]  G. Brown,et al.  Reversal of nitric oxide-, peroxynitrite- and S-nitrosothiol-induced inhibition of mitochondrial respiration or complex I activity by light and thiols. , 2000, Biochimica et biophysica acta.

[4]  L. Brundin,et al.  Neuroprotection by selective inhibition of inducible nitric oxide synthase after experimental brain contusion. , 2006, Journal of neurotrauma.

[5]  Michael R Hamblin,et al.  Role of Low‐Level Laser Therapy in Neurorehabilitation , 2010, PM & R : the journal of injury, function, and rehabilitation.

[6]  I. M. Catalano,et al.  Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by helium‐neon laser , 1984, FEBS letters.

[7]  Da Xing,et al.  Measuring dynamics of caspase‐3 activity in living cells using FRET technique during apoptosis induced by high fluence low‐power laser irradiation , 2005, Lasers in surgery and medicine.

[8]  Nick Lane,et al.  Power games , 2006 .

[9]  R. O. Poyton,et al.  Low intensity light stimulates nitrite-dependent nitric oxide synthesis but not oxygen consumption by cytochrome c oxidase: Implications for phototherapy. , 2011, Journal of photochemistry and photobiology. B, Biology.

[10]  T. Karu,et al.  Primary and secondary mechanisms of action of visible to near-IR radiation on cells. , 1999, Journal of photochemistry and photobiology. B, Biology.

[11]  Jieli Chen,et al.  Low-Level Laser Therapy Applied Transcranially to Rats After Induction of Stroke Significantly Reduces Long-Term Neurological Deficits , 2006, Stroke.

[12]  D. Xing,et al.  The reactive oxygen species–Src–Stat3 pathway provokes negative feedback inhibition of apoptosis induced by high‐fluence low‐power laser irradiation , 2010, The FEBS journal.

[13]  Jackson Streeter,et al.  810 nm Wavelength light: An effective therapy for transected or contused rat spinal cord , 2009, Lasers in surgery and medicine.

[14]  S. Vincent.  Nitric oxide neurons and neurotransmission , 2010, Progress in Neurobiology.

[15]  Marc Fisher,et al.  Infrared Laser Therapy for Ischemic Stroke: A New Treatment Strategy: Results of the NeuroThera Effectiveness and Safety Trial–1 (NEST-1) , 2007, Stroke.

[16]  Amir Oron,et al.  low-level laser therapy applied transcranially to mice following traumatic brain injury significantly reduces long-term neurological deficits. , 2007, Journal of neurotrauma.

[17]  A. Russo-Neustadt,et al.  Norepinephrine and nitric oxide promote cell survival signaling in hippocampal neurons. , 2010, European journal of pharmacology.

[18]  Michael R. Hamblin,et al.  Low-Level Laser Therapy Activates NF-kB via Generation of Reactive Oxygen Species in Mouse Embryonic Fibroblasts , 2011, PloS one.

[19]  D. Butterfield,et al.  Nitric oxide in the central nervous system: neuroprotection versus neurotoxicity , 2007, Nature Reviews Neuroscience.

[20]  Michael R. Hamblin,et al.  Biphasic Dose Response in Low Level Light Therapy , 2009, Dose-response : a publication of International Hormesis Society.

[21]  P. Lapchak,et al.  Taking a light approach to treating acute ischemic stroke patients: Transcranial near-infrared laser therapy translational science , 2010, Annals of medicine.

[22]  U. Oron,et al.  Ga-As (808 nm) laser irradiation enhances ATP production in human neuronal cells in culture. , 2007, Photomedicine and laser surgery.

[23]  R. D. de Haan,et al.  Wound Healing in Cell Studies and Animal Model Experiments by Low Level Laser Therapy; Were Clinical Studies Justified? A Systematic Review , 2002, Lasers in Medical Science.

[24]  R. O. Poyton,et al.  Therapeutic photobiomodulation: nitric oxide and a novel function of mitochondrial cytochrome c oxidase. , 2011, Discovery medicine.

[25]  B. Fanburg,et al.  Reactive oxygen species in cell signaling. , 2000, American journal of physiology. Lung cellular and molecular physiology.

[26]  Paul A. Lapchak,et al.  Transcranial near infrared laser treatment (NILT) increases cortical adenosine-5′-triphosphate (ATP) content following embolic strokes in rabbits , 2010, Brain Research.

[27]  Michael R. Hamblin,et al.  The role of nitric oxide in low level light therapy , 2008, SPIE BiOS.

[28]  P. Brookes,et al.  Calcium, ATP, and ROS: a mitochondrial love-hate triangle. , 2004, American journal of physiology. Cell physiology.

[29]  R. Waynant,et al.  Light promotes regeneration and functional recovery and alters the immune response after spinal cord injury , 2005, Lasers in surgery and medicine.

[30]  R P Franke,et al.  Biostimulatory windows in low-intensity laser activation: lasers, scanners, and NASA's light-emitting diode array system. , 2001, Journal of clinical laser medicine & surgery.

[31]  H. Abrahamse,et al.  Effect of multiple exposures of low-level laser therapy on the cellular responses of wounded human skin fibroblasts. , 2006, Photomedicine and laser surgery.

[32]  Heidi Abrahamse,et al.  The role of laser fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium‐neon laser irradiation , 2006, Lasers in surgery and medicine.

[33]  Ji-yao Chen,et al.  Effects of Low Power Laser Irradiation on Intracellular Calcium and Histamine Release in RBL‐2H3 Mast Cells , 2007, Photochemistry and photobiology.

[34]  S. Jović,et al.  Low-level laser therapy for acute neck pain with radiculopathy: a double-blind placebo-controlled randomized study. , 2010, Pain medicine.

[35]  R. Albertini,et al.  Anti‐inflammatory effects of low‐level light emitting diode therapy on achilles tendinitis in rats , 2010, Lasers in surgery and medicine.

[36]  Chukuka S. Enwemeka,et al.  A Meta-analysis of the Efficacy of Laser Phototherapy on Pain Relief , 2010, The Clinical journal of pain.

[37]  Anita Saltmarche,et al.  Improved cognitive function after transcranial, light-emitting diode treatments in chronic, traumatic brain injury: two case reports. , 2011, Photomedicine and laser surgery.

[38]  Hsin-Su Yu,et al.  Helium-neon laser irradiation stimulates cell proliferation through photostimulatory effects in mitochondria. , 2007, The Journal of investigative dermatology.

[39]  Y. Koyama,et al.  The effect of low reactive-level laser therapy (LLLT) with helium-neon laser on operative wound healing in a rat model. , 2007, The Journal of veterinary medical science.

[40]  Amir Oron,et al.  Transcranial application of low‐energy laser irradiation improves neurological deficits in rats following acute stroke , 2006, Lasers in surgery and medicine.

[41]  Martin Bienengraeber,et al.  Near infrared light protects cardiomyocytes from hypoxia and reoxygenation injury by a nitric oxide dependent mechanism. , 2009, Journal of molecular and cellular cardiology.

[42]  E. Viikari-Juntura,et al.  Low-level laser therapy for neck pain , 2010, The Lancet.

[43]  R Lubart,et al.  Effect of light on calcium transport in bull sperm cells. , 1992, Journal of photochemistry and photobiology. B, Biology.

[44]  Da Xing,et al.  LPLI inhibits apoptosis upstream of Bax translocation via a GSK‐3β‐inactivation mechanism , 2010, Journal of cellular physiology.

[45]  T. Karu,et al.  Cellular effects of low power laser therapy can be mediated by nitric oxide , 2005, Lasers in surgery and medicine.

[46]  G. Brewer.  Serum‐free B27/neurobasal medium supports differentiated growth of neurons from the striatum, substantia nigra, septum, cerebral cortex, cerebellum, and dentate gyrus , 1995, Journal of neuroscience research.

[47]  G. Baxter,et al.  Laser photobiomodulation of wound healing: a review of experimental studies in mouse and rat animal models. , 2010, Photomedicine and laser surgery.

[48]  M. Dyson,et al.  EFFECT OF LIGHT ON CALCIUM UPTAKE BY MACROPHAGES , 1990 .

[49]  Luis De Taboada,et al.  Transcranial laser therapy attenuates amyloid-β peptide neuropathology in amyloid-β protein precursor transgenic mice. , 2011, Journal of Alzheimer's disease : JAD.

[50]  D. Lu,et al.  Upregulation of neurogenesis and reduction in functional deficits following administration of DEtA/NONOate, a nitric oxide donor, after traumatic brain injury in rats. , 2003, Journal of neurosurgery.

[51]  Roberta Chow.  Laser acupuncture studies should not be included in systematic reviews of phototherapy. , 2006, Photomedicine and laser surgery.

[52]  J. C. Morales-Medina,et al.  Alterations in dendritic morphology of hippocampal neurons in adult rats after neonatal administration of N‐omega‐nitro‐L‐arginine , 2007, Synapse.

[53]  C. Martino,et al.  Stimulation of ATP synthesis via oxidative phosphorylation in wheat mitochondria irradiated with Helium‐Neon laser , 1996, Biochemistry and molecular biology international.

[54]  R. J. Miller,et al.  Multiple calcium channels and neuronal function. , 1987, Science.

[55]  R. Lavi,et al.  Photochemistry and photobiology of light absorption by living cells. , 2006, Photomedicine and laser surgery.

[56]  P. Pratt,et al.  Enhancement of Nitric Oxide Release from Nitrosyl Hemoglobin and Nitrosyl Myoglobin by Red/Near Infrared Radiation: Potential Role in Cardioprotection , 2009, Journal of molecular and cellular cardiology.

[57]  Paul J Mills,et al.  Low-intensity light therapy: exploring the role of redox mechanisms. , 2008, Photomedicine and laser surgery.

[58]  Stefano Geuna,et al.  Phototherapy promotes regeneration and functional recovery of injured peripheral nerve , 2004, Neurological research.

[59]  D. Muller,et al.  Dendritic spine formation and stabilization , 2009, Current Opinion in Neurobiology.

[60]  Da Xing,et al.  High fluence low‐power laser irradiation induces apoptosis via inactivation of Akt/GSK3β signaling pathway , 2011, Journal of cellular physiology.

[61]  P. Brookes,et al.  Mitochondrial H(+) leak and ROS generation: an odd couple. , 2005, Free radical biology & medicine.

[62]  Michael R. Hamblin,et al.  Role of reactive oxygen species in low level light therapy , 2009, BiOS.

[63]  T. Smith,et al.  J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. , 1991, Biochemistry.