An Aligned Carbon Nanotube Biosensor for DNA Detection

This paper reports the design and fabrication of a biosensor based on aligned single wall carbon nanotubes (SWCNTs) with integrated single-strand DNAs (ssDNA). This ultra-sensitive biosensor provides label-free real-time electronic detection of DNA hybridization between surface-immobilized ssDNA and target ssDNA. Hybridization kinetics between complementary and target ssDNA nucleotide base pairs result in a local charge generation between base pairs that is injected into the SWCNTs resulting in a detectable change in SWCNT electrical conductance. This conductance change is amplified electrically through the integration of the functionalized SWCNTs as the semi-conductive channel in a silicon-silicon oxide based field effect transistor (FET). Based on previous Langmuir DNA kinetics calculations, the projected sensitivity level of the SWCNT-DNA sensor is considerably higher than traditional fluorescent and hybridization assays.

[1]  Arup K Chakraborty,et al.  Hybridization dynamics of surface immobilized DNA. , 2004, The Journal of chemical physics.

[2]  Itamar Willner,et al.  Biomolecule-functionalized carbon nanotubes: applications in nanobioelectronics. , 2004, Chemphyschem : a European journal of chemical physics and physical chemistry.

[3]  A. Woolley,et al.  Deposition and Characterization of Extended Single-Stranded DNA Molecules on Surfaces , 2001 .

[4]  R. Deaton,et al.  Development of an electrically addressable DNA-based aligned multi-walled carbon nanotube nanosensor , 2005, 5th IEEE Conference on Nanotechnology, 2005..

[5]  E. Tu,et al.  Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[6]  P. Sorger,et al.  Electronic detection of DNA by its intrinsic molecular charge , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[7]  Russell Deaton,et al.  In situ fluorescence microscopy visualization and characterization of nanometer-scale carbon nanotubes labeled with 1-pyrenebutanoic acid, succinimidyl ester , 2006 .

[8]  B. Fishbine.  Carbon Nanotube Alignment and Manipulation Using Electrostatic Fields , 1996 .

[9]  E. Braun,et al.  DNA-Templated Carbon Nanotube Field-Effect Transistor , 2003, Science.

[10]  K. Ueno,et al.  Anodization of electrolytically polished Ta surfaces for enhancement of carrier injection into organic field-effect transistors , 2005 .

[11]  M. Lundstrom,et al.  Ballistic carbon nanotube field-effect transistors , 2003, Nature.

[12]  R. Ruoff,et al.  Tensile loading of ropes of single wall carbon nanotubes and their mechanical properties , 2000, Physical review letters.

[13]  DeWitt G. Ong.  Modern Mos Technology: Processes, Devices, and Design , 1984 .

[14]  R. Smalley,et al.  Electrical and thermal transport properties of magnetically aligned single wall carbon nanotube films , 2000 .

[15]  H. Morgan,et al.  Ac electrokinetics: a review of forces in microelectrode structures , 1998 .

[16]  Miss A.O. Penney.  (b) , 1974, The New Yale Book of Quotations.

[17]  Herbert Shea,et al.  Single- and multi-wall carbon nanotube field-effect transistors , 1998 .