Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3

Synthesis of silver nanoparticles using α-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10–25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.

[1]  G. Southam,et al.  The occurrence of sulfur and phosphorus within bacterially derived crystalline and pseudocrystalline octahedral gold formed in vitro , 1996 .

[2]  Nelson Durán,et al.  Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains , 2005, Journal of nanobiotechnology.

[3]  Paul Mulvaney,et al.  Surface Plasmon Spectroscopy of Nanosized Metal Particles , 1996 .

[4]  R. Mehra,et al.  Metal ion resistance in fungi: Molecular mechanisms and their regulated expression , 1991, Journal of cellular biochemistry.

[5]  M. Eftink,et al.  Fluorescence quenching studies with proteins. , 1981, Analytical biochemistry.

[6]  Sudhakar R. Sainkar,et al.  Fungus-mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: a novel biological approach to nanoparticle synthesis , 2001 .

[7]  S. Macnaughton,et al.  Developments in terrestrial bacterial remediation of metals. , 1999, Current opinion in biotechnology.

[8]  K. Weber,et al.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. , 1969, The Journal of biological chemistry.

[9]  C. Granqvist,et al.  Biologically Produced Silver–Carbon Composite Materials for Optically Functional Thin‐Film Coatings , 2000 .

[10]  R. Kumar,et al.  Extracellular Synthesis of Gold Nanoparticles by the Fungus Fusarium oxysporum , 2002, Chembiochem : a European journal of chemical biology.

[11]  R. Murray,et al.  Sites of metal deposition in the cell wall of Bacillus subtilis , 1980, Journal of bacteriology.

[12]  W. Stickle,et al.  Handbook of X-Ray Photoelectron Spectroscopy , 1992 .

[13]  Satyajyoti Senapati,et al.  Enzyme mediated extracellular synthesis of CdS nanoparticles by the fungus, Fusarium oxysporum. , 2002, Journal of the American Chemical Society.

[14]  C. Stoscheck,et al.  Quantitation of protein. , 1990, Methods in enzymology.

[15]  Stephen Mann,et al.  Biomimetic Materials Chemistry , 1995 .

[16]  C. N. R. Rao,et al.  Science and technology of nanomaterials: current status and future prospects , 2001 .

[17]  Kumar,et al.  Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum , 2003 .

[18]  M. Sastry.  Bioreduction of AuCl‐4 Ions by the Fungus, Verticillium sp. and Surface Trapping of the Gold Nanoparticles Formed. , 2001 .

[19]  T Appenzeller,et al.  The man who dared to think small. , 1991, Science.

[20]  F. D. Snell,et al.  Colorimetric Methods Of Analysis , 1950 .

[21]  Sudhakar R. Sainkar,et al.  BIOREDUCTION OF AUCL4− IONS BY THE FUNGUS, VERTICILLIUM SP. AND SURFACE TRAPPING OF THE GOLD NANOPARTICLES FORMED , 2001 .

[22]  C. Granqvist,et al.  Bacteria as workers in the living factory: metal-accumulating bacteria and their potential for materials science. , 2001, Trends in biotechnology.

[23]  E Olsson,et al.  Silver-based crystalline nanoparticles, microbially fabricated. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[24]  M. Steigerwald,et al.  Biosynthesis of cadmium sulphide quantum semiconductor crystallites , 1989, Nature.