Characteristics of Dietary Fatty Acids Isolated from Historic Dental Calculus of the 17th- and 18th-Century Inhabitants of the Subcarpathian Region (Poland)

Dental calculus analysis can be a valuable source of archaeological knowledge, since it preserves not only microbial and host biomolecules but also dietary and environmental debris, as well as metabolic products likely originating from dietary and craft activities. Here we described GC-MS analysis of a set of historic dental calculus samples from the front teeth of the mandibles of seven individuals found in 17th- and 18th-century graves in the city of Rzeszow, located in South-eastern Poland. We have found that only saturated fatty acids, which are characteristic for fats of animal origin, were present in the tested samples. Our preliminary results indicate that the diet of modern-period inhabitants of Rzeszow was rich in animal products, such as meat and dairy products.

[1]  O. Rickards,et al.  A multidisciplinary approach for investigating dietary and medicinal habits of the Medieval population of Santa Severa (7th-15th centuries, Rome, Italy) , 2020, PloS one.

[2]  James A. Fellows Yates,et al.  Microbial differences between dental plaque and historic dental calculus are related to oral biofilm maturation stage , 2019, Microbiome.

[3]  C. Warinner,et al.  Proteomic evidence of dietary sources in ancient dental calculus , 2018, Proceedings of the Royal Society B: Biological Sciences.

[4]  Cecil M. Lewis,et al.  Differential preservation of endogenous human and microbial DNA in dental calculus and dentin , 2018, Scientific Reports.

[5]  M. Noonan,et al.  Normalizing Gas‐Chromatography–Mass Spectrometry Data: Method Choice can Alter Biological Inference , 2018, BioEssays : news and reviews in molecular, cellular and developmental biology.

[6]  Cecil M. Lewis,et al.  The dental calculus metabolome in modern and historic samples , 2017, Metabolomics.

[7]  M. de la Rasilla,et al.  Neanderthals, trees and dental calculus: new evidence from El Sidrón , 2016, Antiquity.

[8]  C. Warinner,et al.  Direct evidence of milk consumption from ancient human dental calculus , 2014, Scientific Reports.

[9]  A. Radini,et al.  Dental Calculus Reveals Unique Insights into Food Items, Cooking and Plant Processing in Prehistoric Central Sudan , 2014, PloS one.

[10]  J. Salimon,et al.  Comparison of Two Derivatization Methods for the Analysis of Fatty Acids and Trans Fatty Acids in Bakery Products Using Gas Chromatography , 2014, TheScientificWorldJournal.

[11]  Jesper V Olsen,et al.  Pathogens and host immunity in the ancient human oral cavity , 2014, Nature Genetics.

[12]  Keith Dobney,et al.  Sequencing ancient calcified dental plaque shows changes in oral microbiota with dietary shifts of the Neolithic and Industrial revolutions , 2013, Nature Genetics.

[13]  C. Lalueza-Fox,et al.  Neanderthal medics? Evidence for food, cooking, and medicinal plants entrapped in dental calculus , 2012, Naturwissenschaften.

[14]  P. Sciulli,et al.  Dirty teeth and ancient trade: Evidence of cotton fibres in human dental calculus from Late Woodland, Ohio , 2011 .

[15]  Philip D Marsh,et al.  Microbiology of dental plaque biofilms and their role in oral health and caries. , 2010, Dental clinics of North America.

[16]  H. L. Månsson,et al.  Fatty acids in bovine milk fat , 2008, Food & nutrition research.

[17]  C. Chow Fatty Acids in Foods and their Health Implications,Third Edition , 2007 .

[18]  Philip D Marsh,et al.  Dental plaque as a biofilm and a microbial community – implications for health and disease , 2006, BMC oral health.

[19]  William Wade,et al.  Unculturable bacteria--the uncharacterized organisms that cause oral infections. , 2002, Journal of the Royal Society of Medicine.

[20]  A. Dieffenbacher,et al.  Lexicon of lipid nutrition (IUPAC Technical Report) , 2001 .

[21]  J. Pokorný,et al.  Food Lipids , 2022 .

[22]  T. Tokarczyk,et al.  Wyniki badań archeologicznych prowadzonych w 2017 roku na ulicy 3 Maja w Rzeszowie, stanowisko 17 , 2018 .

[23]  J. Rogóż,et al.  Nowożytne cmentarzysko z Placu Farnego oraz pochówki przy kościele Świętego Krzyża w Rzeszowie – wstępne informacje z badań antropologicznych , 2018 .

[24]  R. Mensink,et al.  Food lipids : chemistry, nutrition, and biotechnology , 2002 .

[25]  J Beare Rogers,et al.  LEXICON OF LIPID NUTRITION , 2001 .

[26]  L. Berglund,et al.  Pentadecanoic acid in serum as a marker for intake of milk fat: relations between intake of milk fat and metabolic risk factors. , 1999, The American journal of clinical nutrition.

[27]  S. Mennell All manners of food , 1985 .

[28]  C. Fadiman Food in History , 1974 .