Microarray analysis of shear stressed endothelial cells.

The cDNA microarray is an extremely beneficial tool for study of differential gene expression in the cardiovascular system. This technique is used in many different applications including drug discovery, environmental science, and the effects of mechanical forces on vascular cell phenotype. The paper reviews work by others, and describes our study on effects of shear stress on vascular endothelial cells. These microarray studies verified earlier findings using Northern and polymerase chain reaction (PCR) analyses in this area; and also found previously unidentified differentially expressed genes, leading to new hypotheses regarding how cells and tissues respond to biochemical and mechanical stimuli.

[1]  G. S. Huang,et al.  Differential gene expression of livers from ApoE deficient mice. , 2000, Life sciences.

[2]  W. März,et al.  Human connective tissue growth factor is expressed in advanced atherosclerotic lesions. , 1997, Circulation.

[3]  H. Moch,et al.  High-throughput tissue microarray analysis to evaluate genes uncovered by cDNA microarray screening in renal cell carcinoma. , 1999, The American journal of pathology.

[4]  N. Roodi,et al.  Cytochrome P450 1B1 (CYP1B1) pharmacogenetics: association of polymorphisms with functional differences in estrogen hydroxylation activity. , 2000, Cancer research.

[5]  P. Davies,et al.  Flow-mediated endothelial mechanotransduction. , 1995, Physiological reviews.

[6]  C. J. Omiecinski,et al.  Expression of cytochrome P450s and microsomal epoxide hydrolase in primary cultures of human umbilical vein endothelial cells. , 1994, Toxicology and applied pharmacology.

[7]  R. Ross.  The pathogenesis of atherosclerosis: a perspective for the 1990s , 1993, Nature.

[8]  M. Papadaki,et al.  Effects of Fluid Shear Stress on Gene Regulation of Vascular Cells , 1997, Biotechnology progress.

[9]  A. Haverich,et al.  Cellular dedifferentiation of endothelium is linked to activation and silencing of certain nuclear transcription factors: implications for endothelial dysfunction and vascular biology , 2000, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[10]  L. Gelbert,et al.  Gene Expression Profile of Antithrombotic Protein C Defines New Mechanisms Modulating Inflammation and Apoptosis* , 2001, The Journal of Biological Chemistry.

[11]  P. Libby,et al.  Transcriptional profile of mechanically induced genes in human vascular smooth muscle cells. , 1999, Circulation research.

[12]  L V McIntire,et al.  Cyclical strain effects on production of vasoactive materials in cultured endothelial cells , 1992, Journal of cellular physiology.

[13]  Larry V. McIntire,et al.  DNA microarray reveals changes in gene expression of shear stressed human umbilical vein endothelial cells , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[14]  L V McIntire,et al.  Differential regulation of protease activated receptor-1 and tissue plasminogen activator expression by shear stress in vascular smooth muscle cells. , 1998, Circulation research.

[15]  M. Goppelt‐Struebe,et al.  Induction of Connective Tissue Growth Factor by Activation of Heptahelical Receptors , 2000, The Journal of Biological Chemistry.

[16]  B. Helmke,et al.  A spatial approach to transcriptional profiling: mechanotransduction and the focal origin of atherosclerosis. , 1999, Trends in biotechnology.

[17]  S. Diamond,et al.  Fluid flow decreases preproendothelin mRNA levels and suppresses endothelin-1 peptide release in cultured human endothelial cells. , 1991, Journal of vascular surgery.

[18]  D. Nebert,et al.  Tissue- and cell type-specific expression of cytochrome P450 1A1 and cytochrome P450 1A2 mRNA in the mouse localized in situ hybridization. , 1999, Biochemical pharmacology.

[19]  B. McManus,et al.  A Comparison of Aorta and Vena Cava Medial Message Expression by cDNA Array Analysis Identifies a Set of 68 Consistently Differentially Expressed Genes, All in Aortic Media , 2000, Circulation research.

[20]  T. Podor,et al.  Homocysteine-induced endoplasmic reticulum stress and growth arrest leads to specific changes in gene expression in human vascular endothelial cells. , 1999, Blood.

[21]  F. Guengerich.  Metabolism of chemical carcinogens. , 2000, Carcinogenesis.

[22]  K. Nguyen,et al.  Cyclic Strain Increases Protease-Activated Receptor-1 Expression in Vascular Smooth Muscle Cells , 2001, Hypertension.

[23]  D. Shelton,et al.  Microarray analysis of replicative senescence , 1999, Current Biology.